Abstract
Alcohol dehydrogenase (E.C. 1.1.1.1. ADH) catalyzes the inter-conversion of an alcohol and an aldehyde with NAD+ as a cofactor. In most higher organisms that have been studied, several different isozymes of ADH are present. The main function of these isozymes is presumed to be catabolic, to degrade various alcohols or sterols. This presumption is based primarily on the substrate preferences of the various isozymes and the absence of a fermentative pathway for alcohol production during glycolysis. In many organisms there is a distinctive tissue specificity in the distribution of different ADH isozymes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bennetzen, J.L. (1979). Ph.D. Thesis. University of Washington.
Berk, A.J. and P.A. Sharp (1978). Spliced early mRNAs of Simian Virus 40. Proc. Nat. Acad. Sci. USA. 75: 1274–1278.
Cameron, J.R., E.Y. Loh, and R.W. Davis (1979). Evidence for transposition of dispersed repetitive DNA families in yeast. Cell, 16: 739–751.
Ciriacy, M. (1975a). Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. I. Isolation and genetic analysis of adh mutants. Mutation Research, 29: 315–332.
Ciriacy, M. (1975b). Genetics of alcohol dehydrogenase in Saccharomyces cerevisiae. II. Two loci controlling synthesis of the glucose-repressible ADHII. Mol. Gen. Genet., 138: 157–164.
Ciriacy, M. (1979). Isolation and characterization of further cis-and trans-acting regulatory elements involved in the synthesis of glucose-repressible alcohol dehydrogenase (ADHII). Mol. Gen. Genet., 176: 427–431.
Ciriacy, M. and V.M. Williamson (1981). Analysis of mutations affecting Ty-mediated gene mediated gene expression in Saccharomyces cerevisiae. Mol. Gen. Genet.
Clark, L. and J. Carbon (1980). Isolation of a yeast centromere and constructure of functional small circular chromosomes.
Denis, C., E.T. Young, and M. Ciriacy (1981). A positive regulatory gene is required for accumulation of functional mRNA for the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J. Mol. Biol.
Errede, B., T.S. Cardillo, F. Sherman, E. Dubois, J. Deschamps, and J.M. Wiame (1980). Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell, 22: 427–436
Farabaugh, P.J. and G.R. Fink (1980). Insertion of the Eukaryotic transposable element Tyl creates a 5-base pair duplication, Nature, 286: 352–356.
Gafner, J. and P. Philippsen (1980). The yeast transposon Tyl generates duplications of target DNA on insertion. Nature, 286: 414–418.
Garel, A., M. Zolan, and R. Axel (1977). Genes transcribed at diverse rates have a similar conformation in chromatin. Proc. Nat. Acad. Sci., USA, 74: 4867–4871.
Holland, M.J., J.L. Holland, G.P. Thill, and K.A. Jackson (1981). The primary structure of two yeast enolase genes. J. Biol. Chem., 256: 1385–1395.
Jornvall, H. (1977). The primary structure of yeast alcohol dehydrogenase. Eur. J. Biochem., 72: 425–442.
Lohr, D. and L. Hereford (1979). Yeast chromatin is uniformly digested by DNase I. Proc. Nat. Acad. Sci., USA, 76: 4285–4288.
Maxam, A.M. and W. Gilbert (1977) PNAS, 74: 550–554.
Montomery, D.L., U.W. Leung, M. Smith, P. Shalet, G. Faye, and B.O. Hall (1980). Isolation and sequence of the gene for iso-2-cytochrome c in Saccharomyces cerivisiae. Proc. Nat. Acad. Sci., USA, 77: 541–545.
Nasmyth, K.A. and S.I. Reed (1980). Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Nat. Acad. Sci., USA. 77: 2119–2123.
Struhyl, K. D.T. Stinchcomb, S. Scherer, and R.W. Davis (1979). High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules. Proc. Nat. Acad. Sci., USA, 76: 1035–1039.
Weintraub, H. and M. Groudine (1976). Chromosomal subunits in active genes have an altered conformation. Science, 193: 848–855.
Williamson, V.M., J. Bennetzen, E.T. Young, K. Nasmyth, and B.D. Hall (1980). Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast. Nature, 283: 214–216.
Williamson, V.M., E.T. Young, and M. Ciriacy (1981). Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell, 23: 605–614.
Wills, C. and J. Phelps (1975). A technique for the isolation of yeast alcohol dehydrogenase mutants with altered substrate specificity. Arch. Biochem. Biophys., 167: 627–637.
Wills, C. and H. Jornvall (1979). The two major isozymes of yeast alcohol dehydrogenase. Eur. J. Biochem., 99: 323–331.
Wu, C. (1980). The 5’ ends of drosophila heat shock genes in chromatin are hypersensitive to DNase I. Nature, 286: 854–860.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Young, T. et al. (1982). The Alcohol Dehydrogenase Genes of the Yeast, Saccharomyces Cerevisiae: Isolation, Structure, and Regulation. In: Hollaender, A., DeMoss, R.D., Kaplan, S., Konisky, J., Savage, D., Wolfe, R.S. (eds) Genetic Engineering of Microorganisms for Chemicals. Basic Life Sciences. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4142-0_26
Download citation
DOI: https://doi.org/10.1007/978-1-4684-4142-0_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-4144-4
Online ISBN: 978-1-4684-4142-0
eBook Packages: Springer Book Archive