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The Initiation Factors

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Protein Biosynthesis in Eukaryotes

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 41))

Abstract

Initiation of protein synthesis is the process whereby the ribosome binds mRNA and the first aminoacyl-tRNA to form an initiation complex which is capable of entering the elongation phase of protein synthesis. The pathway is complex and involves numerous steps: First, the 80S ribosome dissociates into 40S and 60S ribosomal subunits; the 40S subunit forms a preinitiation complex with methionyl-tRNA and mRNA; this is joined by the 60S subunit to complete formation of the 80S initiation complex. During these steps, two critical events occur: the ribosome selects for translation a specific mRNA from among numerous species of mRNAs; and the methionyl-tRNA interacts with a specific initiator site on the mRNA to assure proper translation in the correct phase. The reactions are promoted or catalyzed by a complex array of initiation factors and involve the hydrolysis of ATP and GTP.

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Authors and Affiliations

  1. Department of Biological Chemistry, School of Medicine, University of California, Davis, CA, 95616, USA

    John W. B. Hershey

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Editors and Affiliations

  1. The Institute for Virology, University of Rome, Rome, Italy

    R. Pérez-Bercoff

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© 1982 Plenum Press, New York

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Hershey, J.W.B. (1982). The Initiation Factors. In: Pérez-Bercoff, R. (eds) Protein Biosynthesis in Eukaryotes. NATO Advanced Study Institutes Series, vol 41. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4124-6_3

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  • DOI: https://doi.org/10.1007/978-1-4684-4124-6_3

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