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Determination of Large Molecular Weights by Fluctuation Spectroscopy with Quasielastic Light Scattering

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Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems

Part of the book series: NATO Advanced Study Institutes Series ((NSSB,volume 73))

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Abstract

A new method is proposed to determine the molecular weight M of large molecules by observing the probability distribution function of the fluctuation of quasielastic light scattering.. The experimental system used is depicted in Figure 1. The quasielastic light I (t) scattered by molecules in a finite volume V is detected by a pnoto-multiplier tube. The photocurrent is amplified, and passed through a low pass filter having a cutoff frequency of ωc. The low pass filter is used to reduce the fluctuation of the photocurrent not associated with the number fluctuation. The probability distribution function of the photocurrent fluctuation is calculated with a correlator and then analyzed with a minicomputer.

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References

  1. K. Ohbayashi, M. Minoda, and H. Utiyama, Macromolecule vol. 14 (1981).

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  2. M. Weissman, H. Schindler, and G. Feher, Proc. Natl. Acad. Sci. USA 73: 2776 (1976).

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  3. S. B. Dubin, G. B. Benedek, F. C. Bancroft, and D. Freifelder, J. Mol. Biol. 54: 547 (1970).

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Author information

Authors and Affiliations

  1. Faculty of Integrated Arts and Sciences, Hiroshima University, Higashisenda-machi, Hiroshima 730, Japan

    Kohji Ohbayashi, Masaharu Minoda & Hiroyasu Utiyama

Authors
  1. Kohji Ohbayashi
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  2. Masaharu Minoda
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  3. Hiroyasu Utiyama
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Editor information

Editors and Affiliations

  1. Massachusetts Institute of Technology, Cambridge, Massachusetts, USA

    Sow-Hsin Chen

  2. State University of New York, Stony Brook, New York, USA

    Benjamin Chu

  3. National Institutes of Health, Bethesda, Maryland, USA

    Ralph Nossal

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© 1981 Plenum Press, New York

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Ohbayashi, K., Minoda, M., Utiyama, H. (1981). Determination of Large Molecular Weights by Fluctuation Spectroscopy with Quasielastic Light Scattering. In: Chen, SH., Chu, B., Nossal, R. (eds) Scattering Techniques Applied to Supramolecular and Nonequilibrium Systems. NATO Advanced Study Institutes Series, vol 73. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4061-4_34

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  • DOI: https://doi.org/10.1007/978-1-4684-4061-4_34

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4063-8

  • Online ISBN: 978-1-4684-4061-4

  • eBook Packages: Springer Book Archive

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