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Various Autoradiographic Methods as a Tool in Cell Growth Studies

  • Chapter
Cell Growth

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 38))

Abstract

In studies in the field of cell biology the autoradiographic method demonstrates radioactivity in tissues and cells by means of a photographic emulsion. The biological specimens, histological sections, smears of cells or squashes of tissue, are covered with a particularly sensitive photographic emulsion. After the appropriate exposure time and photographic development silver grains are found in the emulsion above those cells or cell structures which are radioactively labeled (for the autoradiographic technique see Schultze, 1969; Amlacher, 1974; Rogers, 1979).

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References

  • Alpen, E. L., Cranmore, D., 1959, Observations on the regulation of erythropoiesis and on cellular dynamics by Fe-59 autoradiography, in: “The kinetics of cellular proliferation,” F. Stohlman Jr.,ed., Grune & Stratton, New York-London, 290.

    Google Scholar 

  • Amlacher, E., 1974, Autoradiographie in Histologie und Cytologie, Fischer, Stuttgart.

    Google Scholar 

  • Antoni, F., Köteles, G. J., Hempel, K. and W. Maurer, 1965, Uber die Eignung verschiedener Fixationen und perchlorsäurehaltiger Lösungen für autoradiographische Untersuchungen des RNS-, DNS- und Proteinstoffwechsels, Histochemie, 5: 210.

    Article  PubMed  CAS  Google Scholar 

  • Baserga, R., Tyler, S. A. and W. E. Kisieleski, 1963, The kinetics of growth of the Ehrlich tumor. A comparative study of the kinetics of cellular proliferation with the use of tritiated thymidine, Arch.Pathol.Lab.Med., 76: 9.

    CAS  Google Scholar 

  • Burholt, D. R., Schultze, B. and W. Maurer, 1976, Mode of Growth of the Jejunal Crypt Cells of the Rat: An Autoradiographic Study Using Double Labelling with 3-H- and 14-C-Thymidine in Lower and Upper Parts of Crypts, Cell Tissue Kinet., 9: 107.

    PubMed  CAS  Google Scholar 

  • Camplejohn, R. S., Schultze, B. and W. Maurer, 1980, An in vivo double labeling study of the subsequent fate of cells arrested in metaphase by vincristine in the JB-1 mouse ascites tumour, Cell Tissue Kinet., 13: 239.

    PubMed  CAS  Google Scholar 

  • Carmona, Th., Kappeler, G. W., Schultze, B. and W. Maurer, Availability time of 3-H- and 14-C-thymidine in the jejunal crypt epithelia of the mouse, in prep.

    Google Scholar 

  • Citoler, P.,Citoler, K., Hempel, K., Schultze, B. and W. Maurer, 1966, Autoradiographische Untersuchungen mit zwölf 3-H- and fünf 14-C-markierten Aminosäuren zur Größe des nucleären und cytoplasmatischen Eiweißstoffwechsels bei verschiedenen Zellarten von Maus und Ratte, Z.Zellforsch., 70: 419.

    Article  PubMed  CAS  Google Scholar 

  • Cleaver, J.E., 1967, Thymidine metabolism and cell kinetics, in: “Frontiers of biology”, Vol.6, Neuberger, A. and E. L. Tatum, eds., North-Holland Publ. Comp., Amsterdam.

    Google Scholar 

  • Cowan, W. M. and M. Cuenod, 1975, The use of axonal transport for studies of neuronal connectivity, Elsevier, Amsterdam, Oxford, New York.

    Google Scholar 

  • Droz, B. and H. Warhawsky, 1963, Relability of the radioautographic technique for the detection of newly synthesized protein, J. Histochern. Cytochem., 11: 426.

    Article  CAS  Google Scholar 

  • Fried, J., 1968, Estimating the median generation time of proliferating cell systems in steady state, Biophys. J., 8: 710.

    Article  PubMed  CAS  Google Scholar 

  • Fried, J., 1970, Mean, geometric mean, or median grain count in cell cycle studies, Exp. Cell Res., 59: 447.

    Article  PubMed  CAS  Google Scholar 

  • Fujita, S., 1962, Kinetics of cell proliferation, Exp. Cell Res., 28: 52.

    Google Scholar 

  • Garweg, G., 1969, Unterschiedliche Verteilung von L-Histidin und L-Prolin im Autoradiogramm des Mäuse-Kleinhirns, Naturwissenschaften, 56: 463.

    Article  PubMed  CAS  Google Scholar 

  • Gerecke, D., 1970, An improved method for the evaluation of DNA synthesis time from the graph of labeled mitoses, Exptl. Cell Res., 62: 487.

    Article  CAS  Google Scholar 

  • Jellinghaus, W., Maidhof, R., Schultze, B. and W. Maurer, 1975, Experimentelle Untersuchungen und zellkinetisehe Berechnungen zur Frage der Synchronisation mit Vincristin in vivo (Mäuseleukämie L 1210, Krypten-Epithelien der Maus ), Z. Krebsforsch., 84: 161.

    Article  CAS  Google Scholar 

  • Jellinghaus, W., Schultze, B. and W. Maurer, 1977, The effect of vincristine on mouse jejunal crypt cells of differing cell age: Double labelling autoradiographic studies using 3-H- and 14-C-Td R, Cell Tissue Kinet., 10: 147.

    PubMed  CAS  Google Scholar 

  • Killmann, S. A., Cronkite, E. P., Fliedner, T. M. and V. P. Bond, 1962, Cell proliferation in multiple myeloma studied with tritiated thymidine in vivo, Lab. Invest., 11: 845.

    PubMed  CAS  Google Scholar 

  • Klein, H. O., Lennartz, K. J., Habicht, W., Eder, M. and R. Gross, 1970, Synchronisation von Ehrlich-Ascites-Tumorzel1 en und ihre Bedeutung bei der Anwendung eines alkylierenden Cytostaticum, Klin. Wschr., 48: 1001.

    Article  PubMed  CAS  Google Scholar 

  • Klein, H. O., Lennartz, K. J., Gross, R., Eder, M. and R. Fischer, 1972, In-vivo- und In-vitro-Untersuchungen zur Zellkinetik und Synchronisation menschlicher Tumorzellen, Dtsch. med. Wschr., 97: 1273.

    Google Scholar 

  • Klein, H. O. and K. J. Lennartz, 1974, Chemotherapy after synchronization of tumor cells, Sem. in Haematol., 11: 203.

    Google Scholar 

  • Klein, H. O., Adler, D., Doering, M., Klein, P. J. and K. J. Lennartz, 1976, Investigations on pharmacologic induction of partial synchronization of tumor cell proliferation: Its relevance for cytostatic therapy, Cancer treatment Rep., 60: 1959.

    CAS  Google Scholar 

  • Korr, H., 1978, Autoradiographische Untersuchungen zur Proliferation verschiedener Zellelemente im Gehirn von Nagern während der prä- und postnatalen Ontogenese, Habilitationsschrift, University of Würzburg.

    Google Scholar 

  • Korr, H., 1980, Proliferation of different cell types in the brain, Adv. Anat. Embryol. Cell Biol., Vol. 61, Springer, Berlin, Heidelberg, New York.

    Book  Google Scholar 

  • Korr, H., Schultze, B. and W. Maurer, 1973, Autoradiographic investigations of glial proliferation in the brain of adult mice. I. The DNA synthesis phase of neuroglia and endothelial cells, J. Comp. Neurol., 150: 169.

    Article  PubMed  CAS  Google Scholar 

  • Korr, H., Schultze, B. and W. Maurer, 1975, Autoradiographic investigations of glial proliferation in the brain of adult mice. II. Cycle time and mode of proliferation of neuroglia and endothelial cells, J.Comp.Neurol., 160: 477.

    Google Scholar 

  • Lang, W., Müller, D. and W. Maurer, 1968, Prozentuale Beteiligung von exogenem Thymidin an der Synthese von DNS-Thymin in Geweben der Maus und in HeLa-Zellen, Exp.Cell Res., 49: 558.

    Article  PubMed  CAS  Google Scholar 

  • Maurer, W., 1972, Methodisches zur autoradiographischen Untersuchung des Eiweißstoffwechsels, Acta histochem., Suppl.-Bd. XII, 65.

    Google Scholar 

  • Maurer, W. and E. Primbsch, 1964, Größe der ß-Selbstabsorption bei der 3-H-Autoradiographie, Exp.Cell Res., 33: 8.

    Article  PubMed  CAS  Google Scholar 

  • Maurer, W. and B. Schultze, 1968, Uberblick über autoradiographische Methoden und Ergebnisse zur Bestimmung von Generationszeiten und Teilphasen von tierischen Zellen mit markiertem Thymidin, Acta histochem., Suppl. 8: 73.

    Google Scholar 

  • Maurer, W., B. Schultze and Th. Carmona, in prep., Tracer-Dosis für exogenes markiertes Thymidin und Beteiligung des exogenen Thymidin an der endogenen DNA-Synthese gemessen an den Krypten.

    Google Scholar 

  • Maurer, W., B. Schultze, A. C. Schmeer and V. Haack, 1972, Autoradiographic studies on the mode of growth in jejunal crypt cells of the mouse, J. Microscopy, 96: 181.

    Article  CAS  Google Scholar 

  • Maidhof, R., Jellinghaus, W., B. Schultze and W. Maurer, 1975, Experimentelle und theoretische Untersuchungen ztir Erzeugung einer teilsynchron proliferierenden Zellpopulat ion mit Vincristin in vivo, Dtsch. med.Wschr., 100: 54.

    PubMed  CAS  Google Scholar 

  • Mendelsohn, M. L., 1960, The gr th fraction: a new concept applied to tumors, Science, 132: 1496.

    Google Scholar 

  • Mendelsohn, M. L., 1962, Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. III. The growth fraction, J. Natl. Cancer Inst., 28: 1015.

    PubMed  CAS  Google Scholar 

  • Niklas, A., Quincke, E., Maurer, W. and H. Neyen, 1958, Messung der Neubildungsraten und biologischen Halbwertszeiten des Eiweißes einzelner Organe und Zellgruppen bei der Ratte, Biochem. Zeitschr., 330: 1.

    Google Scholar 

  • Oehlert, W., Schultze, B. and W. Maurer, 1958, Autoradiographische Untersuchung der Größe des Eiweißstoffwechsels der verschiedenen Zellen des Zentralnervensystems, Beitr. pathol. Anat. 119: 343.

    PubMed  CAS  Google Scholar 

  • Quastler, H. and F. G. Sherman, 1959, Cell population kinetics in the intestinal epithelium of the mouse, Exp.Cell Res., 17: 420.

    Article  PubMed  CAS  Google Scholar 

  • Rogers, A. W., 1979, Techniques of autoradiography, 3rd ed., Elsevier/North-Holland Biomedical Press, Amsterdam,New York,Oxford.

    Google Scholar 

  • Schneider, G. and W. Maurer, 1963, Autoradiographische Untersuchung über den Einbau von H-3-Cytidin in die Kerne einiger Zell-arten der Maus und über den Einfluß des Fixationsmittels auf dieH-3-Aktivitat, Acta histochem., 15: 171.

    PubMed  CAS  Google Scholar 

  • Schultze, B., 1968, Die Orthologie und Pathologie des Nucleinsäure-Eiweißstoffwechsels der Zelle im Autoradiogramm, in: “Handbuch der allgemeinen Pathologie”, Bd. 11/5, H. W. Altmann, F. Büchner, H. Cottier, G. Holle, W. Letterer, W. Masshoff, H. Meessen, F. Roulet, G. Seifert, G. Siebert and A. Studer, eds., Springer, Berlin-Heidelberg-New York, 466.

    Google Scholar 

  • Schultze, B., 1969, Autoradiography at the cellular level, in: “Physical techniques in biological research” 2nd edn, Vol. III b, A. W. Pollister, ed., Academic Press, New York, London.

    Google Scholar 

  • Schultze, B. and W. Maurer, 1963, Größe der RNS-Synthese in Nukleolus und Karyoplasma bei einigen Zellarten der Maus, Z. Zell-forsch., 60: 387.

    Article  CAS  Google Scholar 

  • Schultze, B. and W. Maurer, 1967, Nuclear and cytoplasmic protein synthesis in various cell types from rats and mice, in: “The control of nuclear activity”, L. Goldstein, ed., Prentice-Hall, Inc., Englewood Cliffs, New Jersey, 319.

    Google Scholar 

  • Schultze, B., W. Oehlert and W. Maurer, 1960, Vergleichende autoradiographische Untersuchung mit H-3, C-14 und S-35-markierten Aminosäuren zur Größe des Eiweißstoffwechsels einzelner Gewebe und Zellarten bei Maus, Ratte und Kaninchen, Beitr. pathol. Anat., 122: 406.

    Google Scholar 

  • Schultze, B., Citoler, P., Hempel, K., Citoler, K. and W. Maurer, 1965, Cytoplasmic protein synthesis in cells of various types and its relation to nuclear protein synthesis, in: “The use of radioautography in investigating protein synthesis”, C. P. Leblond and K. B. Warren, eds., Symp. Int. Soc. Cell Biol., Vol. IV, 107, Academic Press, New York, London.

    Google Scholar 

  • Schultze, B., Haack, V., Schmeer, A.C. and W. Maurer, 1972 a, Autoradiographic investigation on the cell kinetics of crypt epithelia in the jejunum of the mouse, Cell Tissue Kinet., 5: 131.

    Google Scholar 

  • Schultze, B., Rabbani, B. and W. Maurer, 1972 b, Blut-Hirn-Schranke und Placentar-Schranke für verschiedene Aminosäuren bei der Maus (Untersuchung mit Ganzkörper-Autoradiographie), Beitr. Path., 147: 352.

    Google Scholar 

  • Schultze, B., Nowak, B. and W. Maurer, 1974, Cycle times of the neural epithelial cells of various types of neuron in the rat. An Autoradiographic study, J. Comp. Neurol., 158: 207.

    Article  PubMed  CAS  Google Scholar 

  • Schultze, B., Maurer, W. and H. Hagenbusch, 1976, A two emulsion autoradiographic technique and the discrimination of the three different types of labelling after double labelling with 3-H- and 14-C-thymidine, Cell Tissue Kinet., 9: 245.

    PubMed  CAS  Google Scholar 

  • Schultze, B., Kellerer, A. M. and W. Maurer, 1979, Transit times through the cycle phases of jejunal crypt cells of the mouse. Analysis in terms of the mean values and the variances, Cell Tissue Kinet., 12: 347.

    PubMed  CAS  Google Scholar 

  • Schultze, B., Jellinghaus, W., Basler, U., Dettmer, J. and W. Maurer, in prep., Experimental studies concerning the so-called synchronization therapy with vincristine.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Institut für Medizinische Strahlenkunde, University of Würzburg, Germany

    B. Maurer-Schultze

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  1. B. Maurer-Schultze
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Editors and Affiliations

  1. Health Sciences Center, Department of Biochemistry and Physiology, Temple University, Philadelphia, Pennsylvania, 19140, USA

    Claudio Nicolini

  2. National Research Council, Genoa, Italy

    Claudio Nicolini

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© 1982 Plenum Press, New York

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Maurer-Schultze, B. (1982). Various Autoradiographic Methods as a Tool in Cell Growth Studies. In: Nicolini, C. (eds) Cell Growth. NATO Advanced Study Institutes Series, vol 38. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4046-1_6

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  • DOI: https://doi.org/10.1007/978-1-4684-4046-1_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-4048-5

  • Online ISBN: 978-1-4684-4046-1

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