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The Relevance of Cell Kinetics in Determining Drug Activity in vitro

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Cell Growth

Part of the book series: NATO Advanced Study Institutes Series ((NSSA,volume 38))

Abstract

Cell cultures provide a rapid, efficient and economic system for initial cytotoxicity screenings of antitumor agents, allowing elucidation of the mode of action of a drug in a controlled, systematic fashion with a high degree of resolution. The main assumption for in vitro studies with chemotherapeutic agents is that the survival response of cultured cells will reflect that of in vivo cells once the drug reaches the neoplastic elements, thus circumventing the pharmacokinetics determinants of tumor response (i.e. absorption, transportation, combination, transformation and degradation). While a direct translation from in vitro to in vivo systems is not possible, many survival responses of proliferating mammalian cells are reasonably similar to the two situations (1–4) provided that strict criteria are established to define cell survival. Drug-induced cell killing is the result of an interplay between the type, extent, and duration of the damaging effect caused by a drug to critical biosynthetic pathways or subcellular structures and the capacity of living elements to bypass or repair such damage. Because of this interplay, a lethally damaged cell may divide several times before the entire progeny perishes from the damage inherited from the single ancestor (5–8).

Supported by Grant CA16763 through the National Large Bowel Cancer Project and Grants CA14528 and CA23272 through the National Cancer Institute, NIH, DHEW.

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Author information

Authors and Affiliations

  1. M.D. Anderson Hospital and Tumor Institute, The University of Texas System Cancer Center, 6723 Bertner, Houston, Texas, 77030, USA

    Benjamin Drewinko & Barthel Barlogie

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  1. Benjamin Drewinko
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  2. Barthel Barlogie
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Editor information

Editors and Affiliations

  1. Health Sciences Center, Department of Biochemistry and Physiology, Temple University, Philadelphia, Pennsylvania, 19140, USA

    Claudio Nicolini

  2. National Research Council, Genoa, Italy

    Claudio Nicolini

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© 1982 Plenum Press, New York

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Drewinko, B., Barlogie, B. (1982). The Relevance of Cell Kinetics in Determining Drug Activity in vitro. In: Nicolini, C. (eds) Cell Growth. NATO Advanced Study Institutes Series, vol 38. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4046-1_35

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  • DOI: https://doi.org/10.1007/978-1-4684-4046-1_35

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