Abstract
The endpoints of in vivo and in vitro assays applied to cells after exposure to a potential oncogenic transforming agent are cellular tumorigenicity and transformation. Tumors are failures of in vivo growth control; transformations are failures of in vitro growth control. Many agents cause tumors in vivo, many agents transform normal cultured cells, and some agents do both. However, even when caused by a single agent, the in vivo and in vitro endpoint assays show only a partial overlap. That is, some but not all tumors will grow as transformed cells in culture, and some but not all in vitro transformants will be tumorigenic on injection into susceptible animals (Shin et al., 1975). Recently, we have described a subset of in vitro phenotypic changes that correlate with in vivo tumorigenicity (Steinberg et al., 1979; Barrett et al., 1979; Pollack, 1981). In this chapter, we will describe recent studies on one of the in vitro changes linked to tumorigenicity, the disruption in organization of cyto-skeletal actin.
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References
Barrett, J. C., Crawford, B. D., Mixter, L. O., Schectman, L. M., Ts’o, P. O. P., and Pollack, R., 1979, Correlation of in vitro properties and tumorigenicity of Syrian hamster cell lines, Cancer Res. 39:1504–1510.
Edelman, G., and Yahara, I., 1976, Temperature-sensitive changes in surface modulating assemblies of fibroblasts transformed by mutants of Rous sarcoma virus, Proc. Natl. Acad. Sci. U.S.A. 73:2047–2051.
Geiger, B., 1979, A 130K protein from chicken gizzard: Its localization at the termini of microfilament bundles in cultured chicken cells, Cell 18:193–205.
Goldman, R. D., Lazarides, E., Pollack, R., and Weber, K., 1975, The distribution of actin in non-muscle cells: The use of actin antibody in the localization of actin within the microfilament bundles of mouse 3T3 cells, Exp. Cell Res. 90:333–344.
Heuser, J. E., and Kirschner, M. W., 1980, Filament organization revealed in platinum replicas of freeze-dried cytoskeletons, J. Cell Biol 86:212–234.
Kahn, P., and Shin, S., 1979, Cellular tumorigenicity in nude mice. II. Test of associations among loss of cell surface fibronectin, anchorage independence and tumor forming ability, J. Cell Biol. 82:1–16.
Kopelovich, L., Conlon, S., and Pollack, R., 1977, Defective organization of actin in cultured skin fibroblasts from individuals with an inherited adenocarcinoma, Proc. Natl. Acad. Sci. U.S.A. 74:3019–3022.
Kopelovich, L., Lipkin, M., Blattner, W. A., Fraumeni, J. F., Lynch, H. T., and Pollack, R., 1980, Organization of actin-containing cables in cultured skin fibroblasts from individuals at high risk of colon cancer, Int. J. Cancer 26:301–308.
Lengsfeld, A. M., Low, I., Wieland, Th., Dancker, P., and Hasselbach, W., 1974, Interaction of phalloidin with actin, Proc. Natl. Acad. Sci. U.S.A. 71:2803–2807.
Macagno, E., Levinthal, C., and Sobel, I., 1979, Three-dimensional computer reconstruction of neurons and neuronal assemblies, Annu. Rev. Biophys. Bioeng. 8:323–351.
McNutt, N., Culp, L., and Black, P., 1973, Revenant cell lines isolated from SV40-transformed cells. IV. Microfilament distribution and cell shape in untransformed, transformed and revenant cells, J. Cell Biol. 56:412–428.
Nicholson, N., Verderame, M., Lipkin, M., and Pollack, R., 1981, F-actin quantitated with Fl-phalloidin in skin fibroblasts of individuals genetically predisposed to colon cancer, in: International Cell Biology, 1980–1981, (H. Sweiger, ed.), pp. 331–335, Springer, Berlin.
Pollack, R., 1981, Hormones, anchorage and oncogenic cell growth, in: Proceedings of 1980 International Symposium on Cancer, Vol. 1 (J. Burchenal, ed.), pp. 501–517, Grune and Stratton, New York.
Pollack, R., and Rifkin, D., 1975, Actin-containing cables within anchorage-dependent rat embryo cells are dissociated by plasmin and trypsin, Cell 6:495–506.
Pollack, R., Green, H., and Todaro, G., 1968, Growth control in cultured cells: Selection of sublines with increased sensitivity to contact inhibition and decreased tumor-producing activity, Proc. Natl. Acad. Sci. U.S.A. 60:126–133.
Pollack, R., Osborn, M., and Weber, K., 1975, Patterns of organization of actin and myosin in normal and transformed non-muscle cells, Proc. Natl. Acad. Sci. U.S.A. 72:994–998.
Pollard, T., and Weihing, R. R., 1974, Actin and myosin and cell movement, CRC Crit. Rev. Biochem. 2:1.
Rifkin, D., Crowe, R., and Pollack, R., 1979, Tumor promotors induce changes in cytoskeletal organization in chick embryo fibroblasts, Cell 18:361–368.
Shin, S., Freedman, V. H., Risser, R., and Pollack, R., 1975, Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro, Proc. Natl. Acad. Sci. U.S.A. 72:4435–4439.
Spudich, J. A., Mockrin, S. C., Brown, S. S., Rubenstein, P. A., and Levinson, A. 1977, The organization and interaction of actin and myosin in non-muscle cells, in: Cell Shape and Architecture, pp. 545–558. Alan R. Liss, New York.
Steinberg, B., and Pollack, R., 1979, Anchorage independence: Analysis of factors affecting the growth and colony formation of wild type and dl 54/59 mutant SV40 transformed line, Virology 99:302–311.
Steinberg, B., Pollack, R., Topp, W., and Botchan, M., 1978, Isolation and characterization of T-antigen negative revenants from a line of transformed rat cells containing one copy of the SV40 genome, Cell 15:19–32.
Steinberg, B., Rifkin, D., Shin, D., Boone, C., and Pollack, R., 1979, Tumorigenicity of revenants from an SV40-transformed line, J. Supramol. Struct. 11:539–546.
Verderame, M., Alcorta, D., Egnor, M., Smith, K., and Pollack, R., 1980, Cytoskeletal F-actin patterns quantitated with Fl-phalloidin in normal and transformed cells, Proc. Natl. Acad. Sci. U.S.A. 77:6624–6628.
Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., and Wieland, Th., 1979, Fluorescent phallotox-in, a tool for the visualization of cellular actin, Proc. Natl. Acad. Sci. U.S.A. 76:4498–4502.
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© 1982 Plenum Press, New York
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Pollack, R., Nicholson, N., Alcorta, D., Verderame, M., Smith, K., Steinberg, B. (1982). Actin Organization as an in Vitro Assay for Tumorigenicity. In: Dowben, R.M., Shay, J.W. (eds) Cell and Muscle Motility. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-4037-9_1
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DOI: https://doi.org/10.1007/978-1-4684-4037-9_1
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