Skip to main content
SpringerLink
Log in

Development of Broad Host-Range Plasmid Vectors

  • Chapter
Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids

Abstract

The majority of cloning vectors developed so far are based on Plasmids or phages with a narrow host-range such as ColEI(pBR3221), P15A(pACYC1842),pSC1013 or phage λ4. These are limited to Escherichia coli or closely related enterobacterial species. For the genetic analysis and manipulation of a wider range of micro-organisms, including those of agricultural, medical, environmental or industrial importance, use has been made of plasmids belonging to the IncP group such as RP4 or RK2. For example RP4 has unique cloning sites for EcoRI, BamHI, HindIII, HpaI and BglII5,6 and has been converted into a SalI cloning vector pRP3017,8. From RK2 a two vehicle system: pRK290 plus pRK2013, has been constructed9. But such vectors remain relatively large, have a low copy number and are therefore unsuitable for some cloning purposes. For ease of manipulation and high gene dosage of the cloned material we need small, high copy number, broad host-range plasmids.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. F Bolivar, R L Rodriguez, P J Greene, M S Betlach, H L Heyneker, H W Boyer, J H Crosa and S Falkow, Gene 2: 95 (1977).

    Article  PubMed  CAS  Google Scholar 

  2. A C Y Chang and S N Cohen, J Bacteriol 134: 1141 (1978).

    PubMed  CAS  Google Scholar 

  3. S N Cohen and A C Y Chang, J Bacteriol 132: 734 (1977).

    PubMed  CAS  Google Scholar 

  4. N E Murray, W J Brammar and K Murray, Molec Gen Genet 150:53(1977)

    Google Scholar 

  5. P T Barth and N J Grinter, J Mol Biol 113: 455 (1977)

    Article  PubMed  CAS  Google Scholar 

  6. A De Picker, M Van Montagu and J Schell in “DNA Insertion Elements, Plasmids and Episomes” Bukhari, Shapiro and Adya, eds, 678 (1977), Cold Spring Harbor Laboratory.

    Google Scholar 

  7. P T Barth, in “Plasmids of Medical, Environmental and Commercial Importance”, K N Timmis and A Pühler eds, 399 (1979), Elsevier/ North Holland.

    Google Scholar 

  8. J D Windass, M J Worsey, E M Pioli, D Pioli, P T Barth, K T Atherton, E C Dart, D Byrom, K Powell and P J Senior,Nature 287: 396 (1980).

    Article  PubMed  CAS  Google Scholar 

  9. G Ditta, S Stanfield, D Corbin and D R Helinski, Proc Natl Acad Sci in press.

    Google Scholar 

  10. H R Smith, G O Humphreys and E S Anderson, Molec Gen Genet 129: 229 (1974).

    Article  PubMed  CAS  Google Scholar 

  11. P T Barth, H Richards and N Datta, J Bacteriol 135: 760 (1978).

    PubMed  CAS  Google Scholar 

  12. N J Grinter and P T Barth, J Bacteriol 128: 394 (1976).

    PubMed  CAS  Google Scholar 

  13. G J Warren, A J Twigg and D J Sherratt, Nature 274: 259 (1978).

    Article  PubMed  CAS  Google Scholar 

  14. R P Novick, R C Clowes, S N Cohen, R Curtiss III, N Datta and S Falkow, Bacteriol Rev 40: 168 (1976).

    PubMed  CAS  Google Scholar 

  15. P T Barth and N J Grinter, J Bacteriol 120: 618 (1974).

    PubMed  CAS  Google Scholar 

  16. J De Gaaff, J H Crosa, F Heffron and S Falkow, J Bacteriol 134: 1117 (1978).

    Google Scholar 

  17. R Meyer, G Boch and J Shapiro, Molec Gen Genet 171: 7 (1979).

    Article  PubMed  CAS  Google Scholar 

  18. F Heffron, C Rubens and S Falkow, Proc Natl Acad Sci 72: 3623 (1975).

    Article  PubMed  CAS  Google Scholar 

  19. P T Barth, N J Grinter and D E Bradley, J Bacteriol 133:43(1978).

    Google Scholar 

  20. G Dougan, M Saul, A Twigg, R Gill and D Sherratt, J Bacteriol 138: 48 (1979).

    PubMed  CAS  Google Scholar 

  21. P J Kretschmer and S N Cohen, J Bacteriol 139: 515 (1979).

    PubMed  CAS  Google Scholar 

  22. J A Nowak and A J Clark, Lunteren Lectures on Molecular Genetics (1979).

    Google Scholar 

  23. N J Panopoulos, B J Staskawicz and D Sandlin in “Plasmids of Medical, Environmental and Commercial Importance”, K N Timmis and A Pühler eds, 365 (1979), Elsevier/North Holland.

    Google Scholar 

  24. Gary Ditta, personal communication.

    Google Scholar 

  25. JoAnne Williams, personal communication.

    Google Scholar 

  26. Tom Schmidthauser, personal communication.

    Google Scholar 

  27. Wolfgang Schuch, personal communication.

    Google Scholar 

  28. Kim Ellis, personal communication.

    Google Scholar 

  29. M Bagdasarian, M M Bagdasarian, S Coleman and K N Timmis, in “Plasmids of Medical, Environmental and Commercial Importance”, K N Timmis and A Pühler eds., 411 (1979), Elsevier/North Holland.

    Google Scholar 

  30. F Gautier and R Bonewald. Molec Gen Genet 178: 375 (1980).

    Article  PubMed  CAS  Google Scholar 

  31. C Rubens, F Heffron and S Falkow, J Bacteriol 128: 425 (1976).

    PubMed  CAS  Google Scholar 

  32. F Heffron, B J McCarthy, H Ohtsubo and E Ohtsubo, Cell 18: 1153 (1979).

    Article  PubMed  CAS  Google Scholar 

  33. A Nordheim and K N Timmis, Lunteren Lectures on Molecular Genetics (1979).

    Google Scholar 

  34. G Dougan, M Saul, A Twigg, R Gill and D J Sherratt, J Bacteriol 138: 48 (1979).

    PubMed  CAS  Google Scholar 

  35. D T Kingsbury and D R Helinski, Biochem Biophys Res Comm 41: 1538 (1970).

    Article  PubMed  CAS  Google Scholar 

  36. N D F Grindley and W S Kelley, Molec Gen Genet 143: 311 (1974).

    Google Scholar 

  37. R Meyer and J Shapiro,EMB0 Workshop on Control of Replication and Partitioning of Bacterial Chromosomes and Plasmids,Leicester (1979).

    Google Scholar 

  38. M Kahn and D R Helinski, Proc Natl Acad Sci 75: 2200 (1978).

    Article  PubMed  CAS  Google Scholar 

  39. David Stalker, personal communication.

    Google Scholar 

  40. F Schöffl and A Pühler, Genet Res Camb. 33: 253 (1979).

    Article  Google Scholar 

  41. R Mattes, H J Burkardt and R Schmitt, Molec Gen Genet.168: 173 (1979).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ICI Corporate Laboratory, The Heath, Runcorn, Cheshire, England

    Peter T. Barth & Lyn Tobin

  2. ICI Joint Laboratory, University of Leicester, Leicester, England

    Geoffrey S. Sharpe

Authors
  1. Peter T. Barth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lyn Tobin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Geoffrey S. Sharpe
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Tufts University School of Medicine, Boston, Massachusetts, USA

    Stuart B. Levy

  2. The University of Texas at Dallas, Richardson, Texas, USA

    Royston C. Clowes

  3. Universidad Nacional Pedro Henriquez Urena, Santo Domingo, Dominican Republic

    Ellen L. Koenig

Rights and permissions

Reprints and permissions

Copyright information

© 1981 Springer Science+Business Media New York

About this chapter

Cite this chapter

Barth, P.T., Tobin, L., Sharpe, G.S. (1981). Development of Broad Host-Range Plasmid Vectors. In: Levy, S.B., Clowes, R.C., Koenig, E.L. (eds) Molecular Biology, Pathogenicity, and Ecology of Bacterial Plasmids. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3983-0_42

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-3983-0_42

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3985-4

  • Online ISBN: 978-1-4684-3983-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation