Reduction of Nitrate and Nitrite in Barley Leaves in Darkness

  • M. Aslam
  • R. C. Huffaker
  • C. C. Delwiche


Reports are contradictory as to whether nitrate and nitrite are reduced in darkness in green leaves. Several workers report that nitrate is not assimilated in darkness in green leaves (Canvin and Atkins, 1974; Sawhney et al., 1978). Taken as evidence for a light requirement also for nitrite reduction is the coupling of nitrite reduction to photosynthetic electron flow at the level of ferredoxin (Miflin, 1974; Neyra and Hageman, 1974). Sadler and Scott (1974) observed that nitrite reduction is almost completely light-dependent and that the process does not require CO2 fixation in barley leaves. Sawhney et al. (1978) reported that nitrate reduction occurs only in light, so accumulation of toxic levels of nitrite in the dark is avoided. It has been proposed that nitrite is not found in darkness under aerobic conditions because O2 inhibits nitrate reduction (Sawhney et al., 1978; Atkins and Canvin, 1975).


Nitrate Reductase Green Leaf Nitrate Reduction Nitrate Reductase Activity Barley Leave 
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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • M. Aslam
  • R. C. Huffaker
    • 1
  • C. C. Delwiche
    • 2
  1. 1.Plant Growth Laboratory and the Department of Agronomy and Range ScienceUniversity of CaliforniaDavisUSA
  2. 2.Department of Land, Air, and Water ResourcesUniversity of CaliforniaDavisUSA

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