Abstract
The metabolites of polyunsaturated fatty acids constitute a large and steadily growing family of compounds, with the classical prostaglandins as the first identified members, and the thromboxanes, prostacyclins and leukotrienes as more recently discovered products. Most kinds of studies in this increasingly complicated area involve detection and often also quantitation of these compounds, which generally occur in extremely small amounts. Thus, sensitive and specific assay methods are needed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
E. Granström and B. Samuelsson, Quantitative measurement of prostaglandins and thromboxanes: General considerations. In: “Advances in Prostaglandin and Thromboxane Research,” Vol. 5, J. C. Frölich ed., Raven Press, New York, (1978), pp. 1–13.
B. Samuelsson, M. Ramberg and C. Sweeley, Quantitative gas chromatography of prostaglandin El at the nanogram level: Use of deuterated carrier and multiple-ion analyzer. Anal. Biochem., 38: 301 (1970).
K. Green, M. Ramberg, B. Samuelsson, M. Smigel and J. C.Frölich, Measurement of prostaglandins, thromboxanes, prostacyclin and their metabolites by gas-liquid chromatography–mass spectrometry. In: “Advances in Prostaglandin and Thromboxane Research”, Vol. 5. J. C. Frölich ed., Raven Press, New York, (1978), pp. 39–94.
K. Green, E. Granström, B. Samuelsson and U. Axen, Methods for quantitative analysis of PGF2a, PGE2, 9a, lla-dihydroxy15-keto-prost-5-enoic acid and 9a,11a,15-trihydroxyprost-5enoic acid from body fluids using deuterated carriers and gas chromatography - mass spectrometry. Anal. Biochem. 54: 434 (1973).
B. Samuelsson, M. Goldyne, E. Granström, M. Ramberg, S. Hammarström and C. Malmsten, Prostaglandins and thromboxanes. Ann. Rev. Biochem., 47: 997 (1978).
E. Granström, Radioimmunoassay of prostaglandins. Prostaglandins, 15: 3 (1978).
E. Granström and H. Kindahl, Radioimmunoassay of prostaglandins and thromboxanes. In: “Advances in Prostaglandin and Thromboxane Research”, Vol. 5. J. C. Frölich ed., Raven Press, New York (1978), pp. 119–210.
M. Hambers, J. Svensson, T. Wakabayashi and B. Samuelsson, Isolation and structure of two prostaglandin endoperoxides that cause platelt aggregation. Proc. Natl. Acad. Sci. USA, 71: 345 (1974).
M. Hamberg, J. Svenssen and B. Samuelsson, Prostaglandin endoperoxides. A new concept concerning the mode of action and release of prostaglandins. Proc. Natl. Acad. Sci. USA, 71: 3824, (1974).
M. Hamberg, J. Svensson and B. Samuelsson, Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc. Natl. Acad. Sci. USA, 72: 2994 (1975).
E. Granström, H. Kindahl and B. Samuelsson, A method for measuring the unstable thromboxane A2: Radioimmunoassay of the derived mono-0-methyl-thromboxane B2. Prostaglandins, 12: 929 (1976).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Plenum Press, New York
About this chapter
Cite this chapter
Granström, E. (1981). Assay Methods for Prostaglandins and Thromboxanes: Gas Chromatographic — Mass Spectrometric Methods and Radioimmunoassay. In: Berti, F., Velo, G.P. (eds) The Prostaglandin System. NATO Advanced Study Institutes Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3896-3_6
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3896-3_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3898-7
Online ISBN: 978-1-4684-3896-3
eBook Packages: Springer Book Archive