Abstract
The dissociated cell culture approach to the analysis of central nervous system (CNS) function begins with a radical disassembly of the system into its single-cell components. Reconstitution of the system must be done in such a way that the reassembled components convincingly capture those neurobiological properties or processes that are of concern to the investigator. In the most general scheme, this would involve separation of the single cell suspension into pure subpopulations corresponding to each major CNS cell type. The intrinsic properties of each cell type and their development with time could be determined by appropriate electrophysiological, morphological, and biochemical studies. Interactions among the different cell types could then be studied in a variety of cultures formed from combinations of the different cell types. Some of these interactions would presumably be specific to particular cell combinations and would contribute to an understanding of the assembly of specifically organized neuronal systems in the brain.
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References
Baccaglini, P.I., 1978, Action potentials of embryonic dorsal root ganglion neurones in Xenopus tadpoles, J. Physiol. (Lond.) 283:585–604.
Banker, G.A., and Cowan, W.M., 1977, Rat hippocampal neurons in dispersed cell culture, Brain Res. 126:397–425.
Banker, G.A., and Cowan, W.M., 1979, Further observations on hippocampal neurons in dispersed cell culture, J. Comp. Neurol. 187:469–494.
Barald, K.F., and Berg, D.K., 1978, Labeling cholinergic neurons in cell culture, Neurosci. Abstr. 4:589.
Barald, K.F., and Berg, D.K., 1979a, Autoradioautographic labeling of spinal cord neurons with high affinity choline uptake in cell culture, Dev. Biol. 72:1–14.
Barald, K.F., and Berg, D.K., 1979b, Ciliary ganglion neurons in cell culture: High affinity choline uptake and autoradiographic choline labeling, Dev. Biol. 72:15–23.
Barker, J.L., and McBurney, R.N., 1979, GABA and glycine may share the same conductance channel on cultured mammalian neurones, Nature 277:234–236.
Barker, J.L., and Ransom, B.R., 1978a, Amino acid pharmacology of mammalian central neurones grown in tissue culture, J. Physiol. (Lond.) 280:331–354.
Barker, J.L., and Ransom, B.R., 1978b, Pentobarbitone pharmacology of mammalian central neurones grown in tissue culture, J. Physiol. (Lond.) 280:355–372.
Beattie, M.S., Bresnahan, J.C., and King, J.S., 1978, Ultrastructural identification of dorsal root primary afferent terminals after anterograde filling with horseradish peroxidase, Brain Res. 153:127–134.
Benda, P., De Vitry, F., Picart, R., and Tixier-Vidal, A., 1975, Dissociated cell cultures from fetal mouse hypothalamus: Patterns of organization and ultrastructural features, Exp. Brain Res. 23:29–47.
Berg, D.K., and Fischbach, G.D., 1978, Enrichment of spinal cord cell cultures with motoneurons, J. Cell Biol. 77:83–98.
Bergey, G.K., Macdonald, R.L., and Nelson, P.G., 1978, Adverse effects of tetrodotoxin on early development and survival of postsynaptic cells in spinal cord cultures, Neurosci. Abstr. 4:601.
Bird, M.M., 1978, Presynaptic and postsynaptic organelles of synapses formed in cultures of previously dissociated mouse spinal cord, Cell Tissue Res. 94:503–511.
Bird, M.M., and James, D.W., 1975, The culture of previously dissociated embryonic chick spinal cord cells on feeder layers of liver and kidney, and the development of paraformaldehye induced fluorescence upon the former, J. Neurocytol. 4:633–646.
Brown, A.G., and Fyffe, R.E.W., 1978, The morphology of group Ia afferent fibre collaterals in the spinal cord of the cat, J. Physiol. (Lond.) 274:111–127.
Brown, A.G., Rose, P.K., and Snow, P.J., 1977, The morphology of hair follicle afferent fibre collaterals in the spinal cord of the cat, J. Physiol. (Lond.) 272:779–797.
Burke, R.E., Walmsley, B., and Hodgson, J.A., 1979, HRP anatomy of group Ia afferent contacts on alpha motoneurones, Brain Res. 160:347–352.
Burry, R.W., and Lasher, R.S., 1975, Uptake of GABA in dispersed cell cultures of postnatal rat cerebellum: An electron microscope autoradiographic study, Brain Res. 88:502–507.
Burry, R.W., and Lasher, R.S., 1978, Electron microscopic autoradiography of the uptake of [3H]GABA in dispersed cell cultures of rat cerebellums. I. The morphology of the GABAergic synapse, Brain Res. 151:1–17.
Calvet, M.C., 1974, Patterns of spontaneous electrical activity in tissue cultures of mammalian cerebral cortex vs. cerebellum, Brain Res. 69:281–295.
Calvet, M.C., Drian, M.J., and Privat, A., 1974, Spontaneous electrical patterns in cultured Purkinje cells grown with an antimitotic agent, Brain Res. 79:285–290.
Castellucci, V.F., Carew, T.J., and Kandel, E.R., 1978, Cellular analysis of long-term habituation of the gill-withdrawal reflex of Aplysia californica, Science 202:1306–1308.
Chan-Palay, V., Yonezawa, T., Yoshida, S., and Palay, S., 1978, γ-Aminobutyric acid receptors visualized in spinal cord cultures by [3H]muscimol autoradiography, Proc. Natl. Acad. Sci. USA 75:6281–6284.
Choi, D.W., Farb, D.H., and Fischbach, G.D., 1977, Chlordiazepoxide selectively augments GABA action in spinal cord cultures, Nature 269:342–344.
Christensen, B.N., and Teubl, W.P., 1978, A comparison of synaptic junction localization on lamprey spinal cord neurons by physiological and morphological methods, Neurosci. Abstr. 4:387.
Crain, S.M., 1956, Resting and action potentials of cultured chick embryo spinal ganglion cells, J. Comp. Neurol. 104:285–329.
Crain, S.M., 1976, Neurophysiologic Studies in Tissue Culture, Raven Press, New York.
Crain, S.M., Bornstein, M.B., and Peterson, E. R., 1968, Maturation of cultured embryonic CNS tissues during chronic exposure to agents which prevent bioelectric activity, Brain Res. 8:363–372.
Crain, S.M., Crain, B., Peterson, E.R., and Simon, E.J., 1978, Selective depression by Opioid Peptides of sensory-evoked dorsal-horn network responses in organized spinal cord cultures, Brain Res. 157:196–201.
Creutzfeldt, O., Lux, H., and Watanabe, S., 1966, Electrophysiology of cortical nerve cells, in: The Thalamus (D. Purpura and M. Yahr, eds.), Columbia University Press, New York
Cullheim, S., and Kellerth, J.-O., 1976, Combined light and electron microscopic tracing of neurons, including axons and synaptic terminals, after intracellular injection of horseradish peroxidase, Neurosci. Lett. 2:307–313.
Cullheim, S., and Kellerth, J.-O., 1978, A morphological study of the axons and recurrent axon collaterals of cat sciatic α-motoneurons after intracellular staining with horseradish peroxidase, J. Comp. Neurol. 178:537–558.
Cullheim, S., Kellerth, J.-O., and Conradi, S., 1977, Evidence for direct synaptic interconnections between cat spinal α-motoneurons via the recurrent axon collaterals: A morphological study using intracellular injection of horseradish peroxidase, Brain Res. 132:1–10.
Dichter, M.A., 1978, Rat cortical neurons in cell culture: Culture methods, cell morphology, electrophysiology, and synapse formation, Brain Res. 149:279–293.
Dichter, M.A., and Fischbach, G.D., 1977, The action potential of chick dorsal root ganglion neurones maintained in cell culture, J. Physiol. (Lond.) 267:281–298.
Dimpfel, W., Neale, J.H., and Habermann, E., 1975, 125I-labelled tetanus toxin as a neuronal marker in tissue cultures derived from embryonic CNS, Naunyn-Schmiedebergs Arch. Pharmacol. 290:329–333.
Dionne, V.E., and Stevens, C.F., 1975, Voltage dependence of agonist effectiveness at the frog neuromuscular junction: Resolution of a paradox, J. Physiol. (Lond.) 251:245–270.
Dudel, J., 1974, Nonlinear voltage dependence of excitatory synaptic current in crayfish muscle, Pflügers Arch. 352:227–241.
Dunlap, K., and Fischbach, G.D., 1978, Neurotransmitters decrease the calcium component of sensory neurone action potentials, Nature 276:837–839.
Edwards, F.R., Redman, S.J., and Walmsley, B., 1976a, Statistical fluctuations in charge transfer at Ia synapses on spinal motoneurons, J. Physiol. (Lond.) 259:665–688.
Edwards, F.R., Redman, S.J., and Walmsley, B., 1976b, Non-quantal fluctuations and transmission failures in charge transfer at Ia synapses on spinal motoneurones, J. Physiol. (Lond.) 259:689–704.
Edwards, F.R., Redman, S.J., and Walmsley, B., 1976c, The effect of polarizing currents on unitary Ia excitatory post-synaptic potentials evoked in spinal motoneurones, J. Physiol. (Lond.) 259:705–723.
Engelhardt, J.K., Ishikawa, K., Lisbin, S.J., and Mori, J., 1976, Neurotrophic effects on passive electrical properties of cultured chick skeletal muscle, Brain Res. 110:170–174.
Farb, D.H., Berg, D.K., and Fischbach, G.D., 1979, Uptake and release of [3H]γ-aminobutyric acid by embryonic spinal cord neurons in dissociated cell culture, J. Cell Biol. 80:651–661.
Federoff, S., and Hertz, L. (eds.), 1977, Cell, Tissue, and Organ Cultures in Neurobiology, Academic Press, New York.
Fields, K.L., Brockes, J.P., Mirsky, R., and Wendon, L.M.B., 1978, Cell surface markers for distinguishing different types of rat dorsal root ganglion cells in culture, Cell 14:43–51.
Fischbach, G.D., 1970, Synaptic potentials recorded in cell cultures of nerve and muscle, Science 169:1331–1333.
Fischbach, G.D., 1972, Synapse formation between dissociated nerve and muscle cells in low density cultures, Dev. Biol. 28:407–429.
Fischbach, G.D., and Dichter, M.A., 1974, Electrophysiologic and morphologic properties of neurons in dissociated chick spinal cord cell cultures, Dev. Biol. 37:100–116.
Fischbach, G. D., and Nelson, P.G., 1977, Cell culture in neurobiology, in: Handbook of Physiology, Sect. 1, The Nervous System, Vol. I (E.R. Kandel, vol. ed.), pp. 719–774, American Physiological Society, Bethesda.
Gähwiler, B.H., 1975, The effects of GABA, picrotoxin and bicuculline on the spontaneous bioelectric activity of cultured cerebellar Purkinje cells, Brain Res. 99:85–95.
Gähwiler, B.H., 1976, Inhibitory action of noradrenaline and cyclic AMP in explants of rat cerebellum, Nature 259:483–484.
Gähwiler, B.H., 1978, Mixed cultures of cerebellum and inferior olive: Generation of complex spikes in Purkinje cells, Brain Res. 145:168–172.
Gähwiler, B.H., Sandoz, P., and Dreifuss, J.J., 1978, Neurones with synchronous bursting discharges in organ cultures of the hypothalamic supraoptic nucleus area, Brain Res. 151:245–253.
Geller, H. M., 1975, Phasic discharge of neurons in long-term cultures of tuberal hypothalamus, Brain Res. 93:511–515.
Geller, H.M., and Woodward, D.J., 1974, Responses of cultured cerebellar neurons to iontophoretically applied amino acids, Brain Res. 74:67–80.
Giller, E. L., Jr., Schrier, B. K., Shainberg, A., Fisk, H.R., and Nelson, P.G., 1973, Choline acetyltransferase activity is increased in combined cultures of spinal cord and muscle cells from mice, Science 182:588–589.
Giller, E.L., Jr., Neale, J.H., Bullock, P.N., Schrier, B.K., and Nelson, P.G., 1977, Choline acetyltransferase activity of spinal cord cell cultures increased by co-culture with muscle and by muscle-conditioned medium, J. Cell Biol. 74:16–29.
Godfrey, E.W., Nelson, P.G., Schrier, B.K., Breuer, A.C., and Ransom, B.R., 1975, Neurons from fetal rat brain in a new cell culture system: A multidisciplinary analysis, Brain Res. 90:1–21.
Ham, R.G., 1965, Clonal growth of mammalian cells in a chemically defined, synthetic medium, Proc. Natl. Acad. Sci. USA 53:288–293.
Hasegawa, S., and Kuromi, H., 1977, Effects of spinal cord and other tissue extracts on resting and action potentials of organ-cultured mouse skeletal muscle, Brain Res. 119:133–140.
Hazum, I., Chang, K.-J., and Cuatrecasas, P., 1979, Opiate (enkephalin) receptors of neuroblastoma cells: Occurrence in clusters on the cell surface, Science 206:1077–1079.
Hendelman, W.J., and Marshall, K.C., 1978, HRP characterization of neurons in organized cultures of cerebellum, Neurosci. Abstr. 4:591.
Hendelman, W.J., Marshall, K.C., Aggerwal, A.S., and Wojtowicz, J.M., 1977, Organization of pathways in cultures of mouse cerebellum, in: Cell, Tissue, and Organ Cultures in Neurobiology (S. Fedoroff and L. Hertz, eds.), pp. 539–554, Academic Press, New York.
Hökfelt, T., Johansson, O., Kellerth, J.-O., Ljungdahl, Å., Nilsson, G., Nygårds, A., and Pernow, B., 1977, Immunohistochemical distribution of substance P, in: Substance P (U.S. von Euler and B. Pernow, eds.), pp. 117–145, Raven Press, New York.
Hösli, L., Andres, P.F., and Hösli, E., 1972, Effects of potassium on the membrane potential of spinal neurones in tissue culture, Pflügers Arch. 333:362–365.
Iles, J.F., 1973, Demonstration of afferent terminations in the cat spinal cord, J. Physiol. (Lond.) 234:22–24P.
Iles, J.F., 1976, Central terminations of muscle afferents on motoneurones in the cat spinal cord, J. Physiol. (Lond.) 262:91–117.
Jankowska, E., Rastad, J., and Westman, J., 1976, Intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells, Brain Res. 105:557–562.
Kandel, E., Spencer, W., and Brinley, F., 1961, Electrophysiology of hippocampal neurons. I. Sequential invasion and synaptic organization, J. Neurophysiol. 24:225–242.
Katz, B., 1966, Nerve, Muscle and Synapse, McGraw-Hill, New York.
Katai, S.T., Kocsis, J.D., Preston, R.J., and Sugimori, M., 1976, Monosynaptic inputs to caudate neurons identified by intracellular injection of horseradish peroxidase, Brain Res. 109:601–606.
Krnjević, K., 1974, Chemical nature of synaptic transmission in vertebrates, Physiol. Rev. 54:418–540.
Kuno, M., 1971, Quantum aspects of central and ganglionic synaptic transmission in vertebrates, Physiol. Rev. 51:647–678.
Kuromi, H., and Hasegawa, S., 1975, Neurotrophic effect of spinal cord extract on membrane potentials of organ-cultured mouse skeletal muscle, Brain Res. 100:178–181.
Lasher, R.S., 1974, The uptake of [3H]GABA and differentiation of stellate neurons in cultures of dissociated postnatal rat cerebellum, Brain Res. 69:235–254.
Lasher, R.S., and Zagon, LS., 1972, The effect of potassium on neuronal differentiation in cultures of dissociated newborn rat cerebellum, Brain Res. 41:482–488.
Li, C., Okujava, V., and Bak, A., 1971, Some electrical measurements of cortical elements and neuronal responses to direct stimulation with particular reference to input resistance, Exp. Neurol. 31:263–276.
Lux, H., and Pollen, D., 1966, Electrical constants on the neurons in the motor cortex of the cat, J. Neurophysiol. 29:207–220.
Macdonald, R.L., and Barker, J.L., 1978, Benzodiazepines specifically modulate GABA-mediated postsynaptic inhibition in cultured mammalian neurones, Nature 271:563–564.
Macdonald, R.L., and Nelson, P.G., 1978, Specific-opiate-induced depression of transmitter release from dorsal root ganglion cells in culture, Science 199:1449–1451.
Macdonald, R.L., Moonen, G., and Nelson, P.G., 1978, Postsynaptic pharmacology of cerebellar neurons in cell culture, Neurosci. Abstr. 4:447.
Martin, A.R., 1977, Junctional transmission. II. Presynaptic mechanisms, in: Handbook of Physiology, Sect. 1, The Nervous System, Vol. I (E.R. Kandel, vol. ed.), pp. 329–355, American Physiological Society, Bethesda.
Masuko, S., Kuromi, H., and Shimada, Y., 1979, Isolation and culture of motoneurons from embryonic chicken spinal cords, Proc. Natl. Acad. Sci. USA 76:3537–3541.
Masurovsky, E.B., Benitez, H.H., and Murray, M.R., 1971, Synaptic development in long-term organized cultures of murine hypothalamus, J. Comp. Neurol. 143:263–278.
Matsuda, Y., Yoshida, S., and Yonezawa, T., 1978, Tetrodotoxin sensitivity and Ca component of action potentials of mouse dorsal root ganglion cells cultured in vitro, Brain Res. 154:69–82.
Matthew, E., Neale, E.A., Nelson, P.G., and Zimmerman, E.A., 1979, Immunocytochemical studies on neuronal peptides in dissociated spinal cord-dorsal root ganglion cell cultures, Neurosci. Abstr. 5:533.
McBurney, R.N., and Barker, J.L., 1978, GABA-induced conductance fluctuations in cultured spinal neurones, Nature 274:596–597.
Messer, A., 1977, The maintenance and identification of mouse cerebellar granule cells in monolayer culture, Brain Res. 130:1–12.
Model, P.G., Bornstein, M.B., Crain, S.M., and Pappas, G.D., 1971, An electron microscopic study of the development of synapses in cultured fetal mouse cerebrum continuously exposed to xylocaine, J. Cell Biol. 49:362–371.
Mudge, A.W., Leeman, S.E., and Fischbach, G.D., 1979, Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration, Proc. Natl. Acad. Sci. USA 76:526–530.
Muller, K.J., and McMahan, U.J., 1976, The shapes of sensory and motor neurones and the distribution of their synapses in ganglia of the leech: A study using intracellular injection of horseradish peroxidase, Proc. R. Soc. Lond. B 194:481–499.
Neale, E.A., Macdonald, R.L., and Nelson, P.G., 1977, Morphology of peroxidase injected and electrophysiologically studied spinal cord neurons in culture, J. Cell Biol. 75:114a.
Neale, E.A., Macdonald, R.L., and Nelson, P.G., 1978a, Intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons, Brain Res. 152:265–282.
Neale, E.A., Moonen, G., Macdonald, R.L., Gibbs, W., and Nelson, P.G., 1978b, Morphology and electrophysiology of cerebellar neurons in cell culture, Neurosci. Abstr. 4:592.
Neale, E.A., Bowers, L.M., and Malley, J.D., 1979, Synaptic ultrastructure of identified neurons: A quantitative analysis, J. Cell Biol. 83:142a.
Neale, J.H., Barker, J.L., Uhl, G.R., and Snyder, S.H., 1978, Enkephalin-containing neurons visualized in spinal cord cell cultures, Science 201:467–469.
Neher, B., and Sakmann, B., 1976, Single-channel currents recorded from membrane of denervated frog muscle fibers, Nature 260:799–802.
Neher, E., Sakmann, B., and Steinbach, J.H., 1978, The extracellular patch clamp: A method for resolving currents through individual open channels in biological membranes, Pflügers Arch. 375:219–228.
Nelson, P.G., 1975, Nerve and muscle cells in culture, Physiol. Rev. 55:1–61.
Nelson, P.G., 1976, Central nervous system synapses in cell culture, Cold Spring Harbor Symp. Quant. Biol. 40:359–371.
Nelson, P.G., and Lux, H.D., 1970, Some electrical measurements of motoneuron parameters, Biophys. J. 10:55–73.
Nelson, P.G., and Peacock, J.H., 1973, Electrical activity in dissociated cell cultures from fetal mouse cerebellum, Brain Res. 61:163–174.
Nelson, P.G., Ransom, B.R., Henkart, M., and Bullock, P.N., 1977, Mouse spinal cord in cell culture. IV. Modulation of inhibitory synaptic function, J. Neurophysiol. 40:1178–1187.
Nelson, P.G., Neale, E.A., and Macdonald, R.L., 1978, Formation and modification of synapses in central nervous system cell cultures, Fed. Proc. 37:2010–2015.
Nelson, P.G., Neale, E.A., Matthew, E., and Zimmerman, E.A., 1980, A presynaptic locus of action for the opiates, in: The Role of Peptides in Neuronal function (J.L. Barker and T.S. Smith, eds.), pp. 727–739, Marcel Dekker, New York.
Oh, T.H., and Markelonis, G.J., 1978, Neurotrophic protein regulates muscle acetylcholinesterase in culture, Science 200:337–339.
Peacock, J.H., 1979, Electrophysiology of dissociated hippocampal cultures from fetal mice, Brain Res. 169:247–260.
Peacock, J.H., Nelson, P.G., and Goldstone, M.W., 1973, Electrophysiologic study of cultured neurons dissociated from spinal cords and dorsal root ganglia of fetal mice, Dev. Biol. 30:137–152.
Peacock, J.H., Rush, D.F., and Mathers, L.H., 1979, Morphology of dissociated hippocampal cultures from fetal mice, Brain Res. 169:231–246.
Pickel, V.M., Reis, D.J., and Leeman, S.E., 1977, Ultrastructural localization of substance P in neurons of rat spinal cord, Brain Res. 122:534–540.
Proshansky, E., and Egger, M.D., 1977, Staining of the dorsal root projection to the cat’s dorsal horn by anterograde movement of horseradish peroxidase, Neurosci. Lett. 5:103–110.
Puck, T.T., Cieciura, S.J., and Robinson, A., 1958, Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects, J. Exp. Med. 108:945–956.
Raff, M.C., Fields, K.F., Hakomori, S., Mirsky, R., Pruss, R.M., and Winter, J., 1979, Cell-type-specific markers for distinguishing and studying neurons and the major classes of glial cells in culture, Brain Res. 174:283–308.
Ransom, B.R., and Holz, R.W., 1977, Ionic determinants of excitability in cultured mouse dorsal root ganglion and spinal cord cells, Brain Res. 136:445–453.
Ransom, B.R., Barker, J.L., and Nelson, P.G., 1975, Two mechanisms for poststimulus hyper-polarisations in cultured mammalian neurones, Nature 265:424–425.
Ransom, B.R., Bullock, P.N., and Nelson, P.G., 1977a, Mouse spinal cord in cell culture. III. Neuronal chemosensitivity and its relationship to synaptic activity, J. Neurophysiol. 40:1163–1177.
Ransom, B.R., Christian, C.N., Bullock, P.N., and Nelson, P.G., 1977b, Mouse spinal cord in cell culture. II. Synaptic activity and circuit behavior, J. Neurophysiol. 40:1151–1162.
Ransom, B.R., Neale, E., Henkart, M., Bullock, P.N., and Nelson, P.G., 1977c, Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties, J. Neurophysiol. 40:1132–1150.
Rastad, J., Jankowska, E., and Westman, J., 1977, Arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase, Brain Res. 135:1–10.
Schlapfer, W.T., Mamoon, A.-M., and Tobias, C.A., 1972, Spontaneous bioelectric activity of neurons in cerebellar cultures: Evidence for synaptic interactions, Brain Res. 45:345–363.
Schultzberg, M., Ebendal, T., Hökfelt, T., Nilsson, G., and Pfenniger, K., 1978, Substance P-like immunoreactivity in cultured spinal ganglia from chick embryos, J. Neurocytol. 7:107–117.
Schwartzkroin, P., 1975, Characteristics of CA1 neurons recorded intracellularly in the hippocampal in vitro slice preparation, Brain Res. 85:423–436.
Scott, B.S., Engelbert, V.E., and Fisher, K.C., 1969, Morphological and electrophysiological characteristics of dissociated chick embryonic spinal ganglion cells in culture, Exp. Neurol. 23:230–248.
Seil, F.J., and Leiman, A.L., 1977, Spontaneous versus driven activity in intracerebellar nuclei: A tissue culture study, Exp. Neurol. 54:110–127.
Snow, P.J., Rose, P.K., and Brown, A.G., 1976, Tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase, Science 191:312–313.
Somjen, G., 1975, Electrophysiology of neuroglia, Annu. Rev. Physiol. 37:163–190.
Sotelo, C., Privat, A., and Drian, M.-J., 1972, Localization of [3H]GABA in tissue culture of rat cerebellum using electron microscopy radioautography, Brain Res. 45:302–308.
Spater, H.W., Novikoff, A.B., Spater, S., and Quintana, N., 1977, Pyridoxal-5-phosphatase: A new enzyme activity of GERL in neurons, J. Cell Biol. 75:114a.
Spencer, W., and Kandel, E., 1961, Electrophysiology of hippocampal neurons. III. Firing level and time constant, J. Neurophysiol. 24:260–271.
Spitzer, N.C., and Lamborghini, J.E., 1976, The development of the action potential mechanism of amphibian neurons isolated in culture, Proc. Natl. Acad. Sci. USA 73:1641–1645.
Stefanelli, A., Cataldi, E., and Ieradi, L.A., 1977, Specific synaptic systems in reaggregated spherules from dissociated chick cerebellum cultivated in vitro, Cell Tissue Res. 182:311–325.
Takahashi, K., 1965, Slow and fast groups of pyramidal tract cells and their respective membrane properties, J. Neurophysiol. 28:908–924.
Takeuchi, A., 1977, Junctional transmission. I. Postsynaptic mechanisms, in: Handbook of Physiology, Sect. 1, The Nervous System, Vol. I (E.R. Kandel, vol. ed.), pp. 295–327, American Physiological Society, Bethesda.
Toran-Allerand, C.D., 1978, The luteinizing hormone-releasing hormone (LH-RH) neuron in cultures of the newborn mouse hypothalamus/preoptic area: Ontogenetic aspects and responses to steroid, Brain Res. 149:257–265.
Trenkner, E., and Sidman, R.L., 1977, Histogenesis of mouse cerebellum in microwell cultures, J. Cell Biol. 75:915–940.
Varon, S., and Raiborn, C.W., Jr., 1969, Dissociation, fractionation, and culture of embryonic brain cells, Brain Res. 12:180–199.
Varon, S., and Raiborn, C., 1971, Excitability and conduction in neurons of dissociated ganglionic cell cultures, Brain Res. 30:83–98.
White, W.F., Snodgrass, S.R., and Dichter, M., 1980, Identification of GABA neurons in rat cortical cultures by GABA uptake and autoradiography, Brain Res. 190:139–152.
Wiesel, T.N., and Hubel, D.H., 1965, Comparison of the effects of unilateral and bilateral eye closure on cortical unit responses in kittens, J. Neurophysiol. 28:1029–1040.
Woodward, W.R., and Lindstrom, S.H., 1977, A potential screening technique for neurotransmitters in the CNS: Model studies in the cat spinal cord, Brain Res. 137:37–52.
Yavin, E., and Menkes, J.H., 1973, The culture of dissociated cells from rat cerebral cortex, J. Cell Biol. 57:232–237.
Yavin, Z., and Yavin, E., 1977, Synaptogenesis and myelinogenesis in dissociated cerebral cells from rat embryo on polylysine coated surfaces, Exp. Brain Res. 29:137–147.
Zipser, B., Crain, S.M., and Bornstein, M.B., 1973, Directly evoked “paroxysmal” depolarizations of mouse hippocampal neurons in synaptically organized explants in long-term culture, Brain Res. 60:489–495.
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Nelson, P.G., Neale, E.A., MacDonald, R.L. (1981). Electrophysiological and Structural Studies of Neurons in Dissociated Cell Cultures of the Central Nervous System. In: Nelson, P.G., Lieberman, M. (eds) Excitable Cells in Tissue Culture. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3803-1_2
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