Abstract
The endothelial cells of the cerebral vessels differ histochemically from those of other organs (1). Cerebral capillaries had been found to be rich in γ glutamyl transpeptidase (γ-GTP) and alkaline phosphatase, enzymes found in the endothelial cells of the gut and renal cells, but not in the capillaries of other organs (2). On the basis of these kinds of observations, it was thought that enzymes found in endothelial cells of the brain may play a role in the function of the blood-brain barrier (BBB) (3). More recently, enzymatic studies of these cells have been facilitated by the simplicity of isolation of cerebral capillaries (4–8), Using this approach, the specific activities of many enzymes in brain capillaries were found to differ from those in brain parenchyma (6,8,9). Studies of hexokinase, lactate dehydrogenase (LDH), adenosine triphosphatase (ATPase) and guanosine triphosphatase (GTPase) in the brain capillaries and parenchyma have shown this difference and indicate that there may be a difference in enzyme function dependent on location (10,11). We tentatively propose that the enzymatic organization of the brain microvasculature functions in the maintenance of the BBB and participates in the transport of substances between blood and brain.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Landers JW, Chason JL, Gonzales JE, et al: Morphology and enzymatic activity of rat cerebral capillaries. Lab Invest 11: 1253–1259, 1962.
Albert Z, Orlowski M, Rzucildo Z, et al: Studies on gamma - glutamyl transpeptidase activity and its histochemical localization in the central nervous system of man and different animal species. Acta Histochem 25: 312–320, 1966.
Björklund A, Falck B, Hromek F, et al: An enzymic barrier mechanism for monoamine precursors in the newly-formed brain capillaries following electrolytic or mechanical lesions. J Neurochem 16: 1605–1608, 1969.
Jo F, Karnushina I: A procedure for the isolation of capillaries from rat brain. Cytobios 8: 41–48, 1973.
Brendel K, Meezan E, Carlson EC: Isolated brain microvessels: A purified, metabolically active preparation from bovine cerebral cortex. Science 185: 953–955, 1974.
Goldstein GW, Wolinsky JS, Csejtey J, et al: Isolation of metabolically active capillaries from rat brain. J Neurochem 25: 715–717, 1975.
Mršulja BB, Mršulja BJ, Fujimoto T, et al: Isolation of brain capillaries: A simplified technique. Brain Res 110: 361–365, 1976.
Djuričić BM, Mršulja BB: Enzymic activity of the brain: Microvessels vs. total brain homogenate. Brain Res 138: 561–564, 1977.
Djuričić BM, Rogac Lj, Spatz M, et al: Brain microvessels: enzymic activités. In, Cervos-Navarro J, Betz E, Ebhardt G, Ferszt R and Wullenweber R: Advances in Neurology, New York, Raven Press, 1978, pp 197–205.
Djuričić BM, Mršulja BB: Brain microvessel hexokinase: Kinetic properties. Experientia 35: 169–171, 1979.
Djuričić BM, Stojanovic T, Mršulja BB: Brain capillary guanosine triphosphatase: A distinction from adenosine triphosphatase. Experientia, in press.
Shonk CE, Boxer GE: Enzyme patterns in human tissues. Cancer Res 24: 709–721, 1964.
Lowry OH, Passonneau JV: A flexible system of enzymatic analysis. New York, Academic Press, 1972.
Lowry OH, Passonneau JV: The relationship between substrates and enzymes of glycolysis in brain. J Biol Chem 239: 31–41, 1964.
Everse J, Kaplan NO: Lactate dehydrogenase: Structure and function. In, Meister A (ed): Advances in Enzymology, New York, John Wiley & Sons, 1973, pp 61–133.
Fisher MA, Hagen DQ, Colein RB: Aminooxy acetic acid: Interactions with gamma-amino-butyric acid and the blood-brain barrier. Science 153: 1668–1670, 1966.
Jo F, Csillik B: Topographic correlation between hemato-encephalic barrier and the Cholinesterase activity of brain capillaries. Exp Brain Res 1: 147–151, 1966.
Jo F, Varkonyi T, Csillik B: Developmental alterations in the histochemical structures of brain capillaries: A histochemical contribution to the problem of the blood-brain barrier. Histochemie 9: 140–148, 1967.
Svendgaard N-Aa, Björklund A, Hardebo J-A: Axonal degeneration associated with a defective blood-brain barrier in cerebral implants. Nature 255: 334–336, 1975.
Lai FM, Udenfriend S, Spector S: Presence of norepinephrine and related enzymes in isolated brain microvessels. Proc Natl Acad Sci USA 72: 4622–4625, 1975.
Hardebo JE, Edvinsson L, Falckm B,: Experimental models for histochemical and chemical studies of the enzymatic blood-brain barrier for amine precursors. In, Cervos-Navarro J (ed): The Cerebral Vessel Wall, New York, Raven Press, 1976, pp 233–244.
Lai FM, Spector S: Studies on the monoamine exodase and catechol-O-methyltransferase of the rat cerebral micro- vessels. Arch Int Pharmacodyn 233: 227–234, 1978.
Mršulja BB, Djuričić BM, Mršulja BJ, et al: Brain microvessels: Effects of ischemia and dihydroergotoxine on enzymic activities. In, Cervos-Navarro J, Betz E, Ebhardt G, Ferszt R, Wullenweber R (eds): Advances in Neurology, New York, Raven Press, 1978, pp 207–213.
Djuričić BM, Mršulja BB: Brain microvessels: Glucose metabolizing enzymes in ischemia and subsequent recovery. In, Mršulja BB, Rakic LjM, Klatzo I, Spatz M (eds): Pathophysiology of Cerebral Energy Metabolism, New York, Plenum Press, 1979, pp 239–252.
Spatz M, Micic D, Fujimoto T, et al: Transport phenomena in cerebral ischemia. In, Mršulja BB, Rakic LjM, Klatzo I, and Spatz M: Pathophysiology of Cerebral Energy Metabolism, New York, Plenum Press, 1979, pp 143–153.
Orlowski M: Possible role of glutathione in transport processes. In, Levy G, Battistin L and Lajtha A (eds): Advances of Experimental Medicine and Biology, New York, Plenum Press, 1976, pp 13–28.
Bertler A, Falck B, Owman Ch, et al: The localization of monoaminergic blood-brain barrier mechanisms. Pharmacol Rev 18: 369–385, 1966.
Kaplan GP, Hartman BK, Creveling CR: Immunohistochemical demonstration of catechol-O-methyl transferase in mammalian brain. Brain Res 167: 241–250, 1979.
Purich DL, Fromm HJ: The kinetics and regulation of rat brain hexokinase. J Biol Chem 246: 3456–3463, 1971.
Lenard JL: Protein biosynthesis. Ann Rev Biochem 40: 409–447, 1971.
Cohen ML, Blume AS, Berkowitz BA: Vascular adenylate cyclase: Role of age and guanine nucleotide activation. Blood Vessels 14: 25–42, 1977.
Goldstein GW: Metabolism of brain capillaries in relation to active ion transport. In, Cervos-Navarro J, Betz E, Ebhardt G, Ferszt R and Wullenweber R (eds): Advances in Neurology, New York, Raven Press, 1978, pp 11–16.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1980 Plenum Press, New York
About this chapter
Cite this chapter
Mršulja, B.B., Djuričić, B.M. (1980). Biochemical Characteristics of Cerebral Capillaries. In: Eisenberg, H.M., Suddith, R.L. (eds) The Cerebral Microvasculature. Advances in Experimental Medicine and Biology, vol 131. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3752-2_3
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3752-2_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3754-6
Online ISBN: 978-1-4684-3752-2
eBook Packages: Springer Book Archive