Regulation of Antigen Binding to T Cells: The Role of Products of Adherent Cells, and the H-2 Restriction of the Antigen Bound
We have described recently that antigen binding to T cells is influenced by a soluble factor of peritoneal adherent cells (MF). It was observed by microscopic autoradiography of radio-labeled antigen bound to nylon wool effluent T cells, that the number of antigen binding T cells increases several fold as a result of incubation with MF for 2 hr before antigen binding. We have shown that this effect is antigen specific and that the target cell of MF action is an Lyt-1+, 2−, 3− cell. It was also observed that H-2 identity is not required between the antigen binding T cells and the adherent cells from which the MF was produced. In contrast the Ir type of the antigen binding T cell enriched population did determine whether increased antigen binding could be observed (5). In a subsequent study we have attempted to define the most important molecules involved in antigen binding to T cells. Alloantisera specific to distinct components controlled by the H-2 complex and purified antibodies against immunoglobulin V regions were used to inhibit antigen binding to T cells. It was found that antigen binding to Lyt-1+, MF sensitive non immune T cells is inhibited by anti Ia, anti idiotype (C3H,SW anti-(T,G)-A--L) and anti-VH antibodies. No inhibition was observed when these cells were treated with anti-H-2K, anti-H-2D and anti-Vγ antibodies (3).
KeywordsAdherent Cell Processed Antigen Antigen Binding Soluble Antigen Cyanogen Bromide
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