Regulation of Antigen Binding to T Cells: The Role of Products of Adherent Cells, and the H-2 Restriction of the Antigen Bound

  • P. Lonai
  • J. Puri
  • M. Zeicher
  • L. Steinman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 121B)


We have described recently that antigen binding to T cells is influenced by a soluble factor of peritoneal adherent cells (MF). It was observed by microscopic autoradiography of radio-labeled antigen bound to nylon wool effluent T cells, that the number of antigen binding T cells increases several fold as a result of incubation with MF for 2 hr before antigen binding. We have shown that this effect is antigen specific and that the target cell of MF action is an Lyt-1+, 2, 3 cell. It was also observed that H-2 identity is not required between the antigen binding T cells and the adherent cells from which the MF was produced. In contrast the Ir type of the antigen binding T cell enriched population did determine whether increased antigen binding could be observed (5). In a subsequent study we have attempted to define the most important molecules involved in antigen binding to T cells. Alloantisera specific to distinct components controlled by the H-2 complex and purified antibodies against immunoglobulin V regions were used to inhibit antigen binding to T cells. It was found that antigen binding to Lyt-1+, MF sensitive non immune T cells is inhibited by anti Ia, anti idiotype (C3H,SW anti-(T,G)-A--L) and anti-VH antibodies. No inhibition was observed when these cells were treated with anti-H-2K, anti-H-2D and anti-Vγ antibodies (3).


Adherent Cell Processed Antigen Antigen Binding Soluble Antigen Cyanogen Bromide 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • P. Lonai
    • 1
  • J. Puri
    • 1
  • M. Zeicher
    • 1
  • L. Steinman
    • 1
  1. 1.Department of Chemical ImmunologyThe Weizmann Institute of ScienceRehovotIsrael

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