In Vivo Activation of Murine Peritoneal Macrophages by Nocardia Water Soluble Mitogen (NWSM)

  • I. Löwy
  • D. Juy
  • C. Bono
  • L. Chedid
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 121B)


The water soluble fraction extracted from various strains of Nocardia, and hereafter referred to as NWSM (12), is a potent B-cell mitogen in several strains of mice (7) including the C3H/He Orl. mice, which are low responders to lipopolysaccharide (LPS) (10), and also in rabbits (6) and in humans (9). Bacterial lipopolysaccharides are also B-cell mitogens and adjuvants (19,14) in mice in vivo. However, in contrast to LPS, when NWSM was administered to mice, it failed to induce in vivo proliferation of splenic lymphocytes, polyclonal activation and appearance of PFC directed against syngeneic, bromelain-treated erythrocytes and thymocytes (18). These findings strongly suggest that the adjuvant activity of NWSM is not necessarily related to in vivo mitogenicity, and that other cells than B lymphocytes may be its primary target cell in vivo. During investigations of NWSM in vivo effects we examined the influence of this adjuvant on murine peritoneal macrophages in comparison to other water soluble adjuvants.


Peritoneal Macrophage Phagocytic Activity Peritoneal Exudate Cell Adjuvant Activity Murine Peritoneal Macrophage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Adam, A., Ciorbaru, R., Petit, J. F. and Lederer, E., Proc. Nat. Acad. Sci. USA, 69 (1972) 851.CrossRefGoogle Scholar
  2. 2.
    Adam, A., Ciorbaru, R., Petit, J. F., Lederer, E., Chedid, L., Lamensans, A., Parant, F., Parant, M., Rosselet, J. P. and Berger, F. M., Infect. Immunity, 7 (1973) 855.Google Scholar
  3. 3.
    Alexander, P. and Evans, R., Nature New Biol., 232 (1971) 76.PubMedGoogle Scholar
  4. 4.
    Allison, A. C., In: Immunopotentiation, (Ed. D. W. Dresser ), Elsevier Excerpta Medica, North Holland Associated Scientific Pub., (1973) 73.Google Scholar
  5. 5.
    Audibert, F., Chedid, L., Lefrancier, P. and Choay, J. Cell Immunol., 21 (1976) 243.PubMedCrossRefGoogle Scholar
  6. 6.
    Bona, C., Chedid, L., Damais, C., Ciorbaru, R., Shek, P. N., Dubiski, S. and Cinander, B., J. Immunol., 114 (1975) 348.PubMedGoogle Scholar
  7. 7.
    Bona, C., Damais, C. and Chedid, L., Proc. Nat. Acad. Sci. USA, 71 (1974) 1602.PubMedCrossRefGoogle Scholar
  8. 8.
    Bona, C., Damais, C., Juy, D. and Ciorraru, R., Abstracts of International Symposium on Bacterial Immunostimulants. Institut Pasteur, Paris, (1974) 19.Google Scholar
  9. 9.
    Brochier, J., Bona, C., Ciorbaru, R., Revillard, J. P. and Chedid, L., J. Immunol., 117 (1976) 1434.PubMedGoogle Scholar
  10. 10.
    Chedid, L., Parant, M., Damais, C., Parant, F., Juy, D. and Galelli, A., Infect. Immunity, 13 (1975) 722.Google Scholar
  11. 11.
    Chiller, J. M., Skidmore, B. J., Morrison, D. C. and Weigle, W. O., Proc. Nat. Acad. Sci. USA, 70 (1973) 2129.PubMedCrossRefGoogle Scholar
  12. 12.
    Ciorbaru, R., Adams, A., Petit, J. F., Lederer, E., Bona, C., and Chedid, L., Infect. Immunity, 11 (1975) 257.Google Scholar
  13. 13.
    Ciorbaru, R., Petit, J. F., Lederer, E., Zissman, E., Bona, C. and Chedid, L., Infect. Immunity, 13 (1976) 1084.Google Scholar
  14. 14.
    Franzl, R. E. and McMaster, P. D., J. Exp. Med., 127 (1968) 1087.PubMedCrossRefGoogle Scholar
  15. 15.
    Glode, L. M., Jacques, A., Mergenhagen, S. E. and Rosenstreich, 0., J. Immunol., 119 (1977) 162.PubMedGoogle Scholar
  16. 16.
    Juy, D. and Chedid, L., Proc. Nat. Acad. Sci. USA, 72 (1975) 4105.CrossRefGoogle Scholar
  17. 17.
    Lefrancier, P., Choay, J., Derrien, M. and Lederman, I., Int. J. Petp. Protein Res., 9 (1977) 249.CrossRefGoogle Scholar
  18. 18.
    L6Wy, I., Bona, C., Ciorbaru, R. and Chedid, L., Immunology, 32 (1977) 975.Google Scholar
  19. 19.
    Melchers, F., Braun, V. and Galanos, C., J. Exp. Med., 142 (1975) 473.PubMedCrossRefGoogle Scholar
  20. 20.
    Merser, C., Sinay, P. and Adams, A., Biochem. Biophys. Res. Com., 59 (1975) 1317.Google Scholar
  21. 21.
    Modolell, M., Luckenbach, G. A., Parant, M. and Munder, P. G., J. Immunol., 113 (1974) 395.PubMedGoogle Scholar
  22. 22.
    Parent, M. and Chedid, L., In: Recent Results in Cancer Research, (Ed. G. Mathé ), Springer Verlag, Heidelberg, 47 (1974) 190.Google Scholar
  23. 23.
    Shands, J. W., Peavy, D. L., Gormus, B. J. and McGaw, J. Infect. Immunol., 9 (1974) 106.Google Scholar
  24. 24.
    Skidmore, B. J., Chiller, J. M., Morrison, D. C. and Weigle, W. 0., J. Immunity, 114 (1975) 770.Google Scholar
  25. 25.
    Unanue, E., Askonas, B. and Allison, A. C., J. Immunol., 103 (1969) 71.PubMedGoogle Scholar
  26. 26.
    Watson, J. and Riblet, R., J. Exp. Med., 140 (1974) 1147.PubMedCrossRefGoogle Scholar
  27. 27.
    Weiner, E. and Levanon, D., Lab. Invest., 19 (1968) 584.Google Scholar
  28. 28.
    Westphal, 0., Luderitz, 0. and Bister, B., Z. Naturforsch., 7b (1952) 148.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • I. Löwy
    • 1
  • D. Juy
    • 1
  • C. Bono
    • 1
  • L. Chedid
    • 1
  1. 1.Inserm, Paris and Groupe de Recherche n° 31 du CNRSInstitut PasteurParisFrance

Personalised recommendations