Restoration of Depressed Antibody Responses of Leukemic Splenocytes Treated with LPS-Induced Factors

  • R. C. Butler
  • H. Friedman
  • A. Nowotny
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 121B)


Lipopolysaccharides (LPS) derived from gram negative bacteria are known to enhace antibody responses to a variety of antigens, including serum proteins and xenogeneic erythrocytes, both in vivo and in vitro (1,10,11). Recent studies in this laboratory have shown that such endotoxins may influence the immune response of splenocytes from Friend leukemia virus (FLV) infected mice which showed markedly impaired immune competence (2,3). The mechanism of action of LPS in enhancing antibody formation by leukemic splenocytes, as well as normal splenocytes, is still unclear. It is not known whether the LPS affects immunity by directly interacting with lymphocytes or by inducing intermediate immunoregulatory or immunostimulatory factors. In the present study attempts were made to determine whether pretreatment of mice with LPS causes the release of factors into the serum which could mediate the adjuvant effect on the in vitro antibody response of normal or leukemia virus immunosuppressed cell cultures.


Antibody Response Spleen Cell Serratia Marcescens Muramyl Dipeptide Spleen Cell Culture 
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Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • R. C. Butler
    • 1
    • 2
  • H. Friedman
    • 1
    • 2
  • A. Nowotny
    • 1
    • 2
  1. 1.Depts. of Microbiology and ImmunologyAlbert Einstein Medical Center and University of PennsylvaniaPhiladelphiaUSA
  2. 2.College of MedicineUniversity of South FloridaTampaUSA

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