Leukocyte Enzyme Assays by Flow Cytophotometry

  • L. S. Kaplow
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 121B)


Flow-cytophotometers are optical electronic devices which measure a variety of physical characteristics of individual cells in suspension as they flow, one at a time, past a laser beam of a particular wavelength. Some instruments measure fluorescent emission at different wavelengths and light scattered from each cell detected at a narrow angle. Other instruments measure both narrow angle light scatter and axial light-loss (extinction) due to impingement of the cell on the light path as it traverses the laser beam. The studies to be described will refer only to instruments of the latter type.


Unstained Cell Light Loss Oscilloscope Screen Average Enzyme Activity Optical Electronic Device 
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  1. 1.
    Ansley, H. and Ornstein, L., Adv. Automated Anal. 1 (1971) 437.Google Scholar
  2. 2.
    Horan, P. K. and Wheeless, L. L., Jr., Sci. 198 (1977) 149.CrossRefGoogle Scholar
  3. 3.
    Kaplow, L. S., Am. J. Clin. Path. 39 (1963) 439.Google Scholar
  4. 4.
    Kaplow, L. S., Acta Cytol. 19 (1975) 358.PubMedGoogle Scholar
  5. 5.
    Kaplow, L. S. and Eisenberg, M., Leukocyte differentiation and enumeration by cytochemical-cytographic analysis, In: First International Symposium on Pulse-Cytophotometry, (Eds. C. A. M. Haanen, H. F. P. Hillen and J. M. C. Wessels ), European Press Medikon, 262 (1975).Google Scholar
  6. 6.
    Kaplow, L. S. and Lerner, E., J. Histochem. Cytochem. 25 (1977) 590.PubMedCrossRefGoogle Scholar
  7. 7.
    Kaplow, L. S. and Soman, S., Fed. Proc. 37: 1234 (1978) 444.Google Scholar
  8. 8.
    Kaplow, L. S., Dauber, H. and Lerner, E., J. Histochem. Cytochem. 24 (1976) 363.PubMedCrossRefGoogle Scholar
  9. 9.
    Kaplow, L. S., Gershman, R., Weiner, I. and Soman, S., J. Histochem. Cytochem. 26 (1978) 214.Google Scholar
  10. 10.
    Peacock, A. C., Brecher, G. and Highsmith, E. M., Am. J. Clin. Path. 29 (1958) 80.PubMedGoogle Scholar
  11. 11.
    Saunders, A. M., Clin. Chem. 18 (1972) 783.PubMedGoogle Scholar
  12. 12.
    Trubowitz, S., Feldman, D., Benate, C. and Kirman, D., Proc. Soc. Exp. Biol. Med. 95 (1957) 35.Google Scholar
  13. 13.
    Zucker-Franklin, D., Arch. Intern. Med. 135 (1975) 55.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • L. S. Kaplow
    • 1
    • 2
  1. 1.Laboratory Service Veterans Administration HospitalWest HavenUSA
  2. 2.Departments of Pathology and Laboratory MedicineYale University School of MedicineNew HavenUSA

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