Abstract
Preovulatory follicles were dissected from ovaries of estrous rabbits. Four follicles were placed in each chamber of a perifusion system. Medium 199, containing 10% rabbit serum and antibiotics, eluted through the chambers at a rate of 1 ml/10 min. Every 6 h for 36 h, 1 µg of LH and 50 ng of FSH were injected into each chamber. P4, T and E2 were measured in the medium. P4 exhibited a peak after each of the injections at 6, 12, 18, 24 and 30 h. E2 and T gave major peaks after the injection at 0 time, with minor peaks after the injection at 6 h; thereafter T and E2 secretion gradually decreased to levels below those of baseline.
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© 1979 Plenum Press, New York
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D’Amato, C., Bahr, J., Stockert, B., Calvo, F., Kesler, D. (1979). Long Term Perifusion of Rabbit Ovarian Follicles. In: Channing, C.P., Marsh, J.M., Sadler, W.A. (eds) Ovarian Follicular and Corpus Luteum Function. Advances in Experimental Medicine and Biology, vol 112. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3474-3_28
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DOI: https://doi.org/10.1007/978-1-4684-3474-3_28
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