Abstract
Theca interna cells were collected from large porcine follicles in the follicular phase of the cycle. The theca interna layer was manually separated from the theca externa, washed thoroughly, occasionally treated with hyaluronidase solution and trypsinized. The resulting cell suspension consisted of approximately 70% theca interna cells, 8% granulosa cells, and 23% nonsteroidogenic cells. In one experiment, cells collected from the granulosa layer and the theca interna of the same follicles were compared. Specific cell types were identified by: 1) staining with Oil Red 0 (ORO) to visualize lipid droplets in the cytoplasm, 2) measuring activity of the Δ 36-hydroxysteroid dehydrogenase (Δ 36-OH SH) enzyme, and 3) quantitating estrogen production by the cultured cells.
Theca interna cells can be distinguished from granulosa cells in suspension prior to culture by the following criteria:
-
1.
more intense staining with ORO,
-
2.
stronger Δ5,36-OH SH activity,
-
3.
larger cell size.
Theca interna cells can be distinguished from granulosa cells by the following parameters:
-
1)
more elongated shape,
-
2)
slower and more chaotic growth,
-
3)
weaker enzyme activity,
-
4)
increased estrogen production.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
L. Bjersing. Acta Pathol. Microbiol. Scand. 55(1962)127.
C. P. Channing. Nature. 210 (1966) 1266.
C. P. Channing. J. Endocr. 43(1969)403.
C. P. Channing. J. Endocr. 45(1969)297.
C. P. Channing. Endocrinology. 87(1970)49.
T. V. Fischer and R. H. Kahn. In Vitro. 7(1972)201.
S. G. Korenman, P. M. Lolc, I. Beceiro, B. M. Sherman, and D. K. Granner. Endocrinology. 93 (1973) 1423.
E. Lacroix, W. Eechaute, and I. Leusen. Steroids. 23(1974)337.
M. Ryle, J. Kent, and J. Boggis. Ann. Biol. Anim. Bioch. Biophys. 13(1973)223.
P. Rondell. Biol. Reprod. 10(1974)199.
C. P. Channing. Endocrinology. 87(1970)156.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1979 Plenum Press, New York
About this chapter
Cite this chapter
Stoklosowa, S., Bahr, J., Gregoraszczuk, E. (1979). Some Morphological and Functional Characteristics of Cells of the Porcine Theca Interna in Tissue Culture. In: Channing, C.P., Marsh, J.M., Sadler, W.A. (eds) Ovarian Follicular and Corpus Luteum Function. Advances in Experimental Medicine and Biology, vol 112. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3474-3_17
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3474-3_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3476-7
Online ISBN: 978-1-4684-3474-3
eBook Packages: Springer Book Archive