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I would like to comment about the lysozyme estimations that Dr. Cole carried out in his study. In my opinion, the method that Dr. Cole used to measure lysozyme is the worst possible. Unfortunately, it is the one that most people use. Lysozyme complexes with mucins, and if you freeze your sample, an irreversible complex will form and precipitate. When you spin down your samples, you lose about 50% of your lysozyme. Furthermore, if you take the supernatant and freeze it, you lose more lysozyme by subsequent freezing and thawing. So the values you get for lysozyme in this manner are not reproducible. The only way to obtain reproducible lysozyme values is to acidify your saliva first to get rid of the mucins, then neutralize it. It can then be stored for a week at -20 C or -4 C or even +4 C at quite stable levels. However, if you do not remove mucins as soon as you collect the saliva, you never get reproducible results. Furthermore, using monocytic leukemia controls is very bad because there are isozymes of lysozyme. People can have different lysozymes which can have different activities, so the only good method that we have to standardize the assay is by using the control that is provided by Kallestadt. This control is uniform and it gives reproducible results.
KeywordsSerum Antibody Teichoic Acid Lipoteichoic Acid Caries Experience Parotid Saliva
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