Abstract
Nitrogen retention of adults who consumed diets in which cereals furnished 6.0 g N and 0.9 g of lysine was improved by increasing lysine to 1.8 g without altering other amino acids. In a second experiment, 70% of the 6.0 g of dietary N was supplied by rice + wheat and 30% by mixtures of amino acids so designed that the total intakes of amino acids were equivalent to those in diets containing 6.0 g N from whole egg (E), egg + potato (EP), rice + wheat (RW), rice + soy (RS), wheat + milk (WM) or corn + beans (CB). Mean N balances of young men in descending order were, g/day: E 0.69 ± 0.23, RS 0.44 ± 0.15, EP 0.43 ± 0.09, WM 0.24 ± 0.16, CB 0.16 ± 0.13 and RW -0.02 ± 0.10. In the same order, these diets provided, g/day: lysine 2.6, 1.9, 2.3, 1.6, 1.4 and 1.0; S-acids 2.2, 1.7, 1.6, 2.2, 1.9 and 2.3; and tryptophan 0.7, 0.4, 0.6, 0.5, 0.3 and 0.4. N balances resulting from diets E, RS and EP did not differ significantly from each other but E was superior to CB and RW (P<0.01). Relative amounts and proportions of the essential amino acids could be varied without altering nitrogen retention until at least one amino acid became limiting. Several patterns of indispensable amino acids therefore may be equally effective in meeting needs of adults, but both amounts and relative proportions should be considered.
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References
Bailey, L.B. and Clark, H.E. (1976). Plasma amino acids and nitrogen retention of human subjects who consumed isonitrogenous diets containing rice and wheat or their constituent amino acids with and without additional lysine. Am. J. Clin. Nutr., 29, 1353–1358.
Bressani, R. and Elias, L.G. (1973). Development of highly nutritious products. In, Man, Food and Nutrition, M. Rechcigl (Editor). CRC Press, Cleveland.
Brewer, M.F., Halvorson, J.D. and Clark, H.E. Nitrogen retention of young men who consumed selected patterns of essential amino acids at a constant nitrogen intake. Am. J. Clin. Nutr. In Press.
Calloway, D.H. (1975). Nitrogen balance of men with marginal intakes of protein and energy. J. Nutr., 105, 914–923.
Clark, H.E., Bailey, L.B. and Brewer, M.F. (1977). Lysine and tryptophan in cereal-based diets for adult human subjects. Am. J. Clin. Nutr., 30, 674–680.
Clark, H.E., Boyd, J.N., Kolski, S.M. and Shannon, B. (1968). Nitrogen retention of adults given variable quantities and proportions of lysine. Am. J. Clin. Nutr., 21, 217–222.
Clark, H.E., Fugate, K. and Allen, P.E. (1967). Effect of four multiples of a basic mixture of essential amino acids on nitrogen retention of adult human subjects. Am. J. Clin. Nutr., 20, 233–242.
Clark, H.E., Howe, J.M., Magee, J.L. and Malzer, J. (1972). Nitrogen balances of adult human subjects who consumed four levels of nitrogen from a combination of rice, milk and wheat. J. Nutr., 102, 1647–1654.
Clark, H.E., Howe, J.M., Shannon, B.M., Carlson, K. and Kolski, S.M. (1970). Requirements of adult human subjects for methionine and cystine. Am. J. Clin. Nutr., 23, 731–738.
Clark, H.E., Moon, W.H., Malzer, J.L., Birt, D.F. and Pang, R.L. (1974). Nitrogen retention of adult human subjects fed varying quantities of tryptophan. J. Nutr., 104, 1121–1126.
Clark, H.E., Yang, S.P., Walton, W. and Mertz, E.T. (1960). Amino acid requirements of men and women. II. Relation of lysine requirement to sex, body size, basal caloric expenditure and creatinine excretion. J. Nutr., 71, 229–234.
FAO. Amino Acid Content of Foods and Biologic Data on Proteins. (1970). FAO Nutritional Studies No. 24, Food and Agriculture Organization of United Nations. Rome.
Graham, G.G. (1971). Methionine or lysine fortification of dietary protein for infants and small children. In, ‘Amino Acid Fortification of Protein Foods’, N.S. Scrimshaw and A.M. Altschul (Editors). M.I.T. Press, Cambridge.
Harper, A.E. (1974). Effects of disproportionate amounts of amino acids. In, ‘Improvement of Protein Nutriture’, Nat’l. Acad. of Sciences, Washington, D.C.
Holt, L.E. and Snyderman, S.E. (1965). Protein and amino acid requirements of infants and children. Nutr. Abst. and Rev., 35, 1–13.
Howe, J.M., Clark, H.E., Tewell, J.E. and Senchak, M.M. (1972). Nitrogen retention of adults fed six grams of nitrogen from combinations of rice, milk, and wheat. Am. J. Clin. Nutr., 25, 559–563.
Kofrânyi, E. (1973). Evaluation of traditional hypotheses on the biological value of proteins. Nutr. Repts. Intnl., 7, 45–50.
Kofrânyi, E., Jekat, F. and Muller-Wecker, H. (1970). The minimum protein requirement of humans, tested with mixtures of whole egg plus potato and maize plus beans. Hoppe Seyler’s Z. Physiol. Chem., 351, 1485–1493.
Kolski, S.M., Shannon, B., Howe, J.M. and Clark, H.E. (1969). Nitrogen balances of adults given leucine and valine. Am. J. Clin. Nutr., 22, 21–26.
Lee, C., Howe, J.M., Carlson, C. and Clark, H.E. (1971). Nitrogen retention of young men fed rice with or without supplementary chicken. Am. J. Clin. Nutr., 24, 318–323.
McArthur, L.G., Kirtley, W.R. and Waife, S.O. (1963). Effect of large doses of L-leucine in animals and man. Am. J. Clin. Nutr., 13, 285–290.
Phipard, E.F. (1974). Protein and amino acids in diets. In, ‘Improvement of Protein Nutriture’. Nat’l. Acad. of Sciences, Washington.
Pradilla, A.G., Harpstead, D.D., Sarria, D., Linares, F.A. and Francis, C.A. (1975). Quality protein maize in human nutrition. In, ‘High-Quality Protein Maize’. Dowden, Hutchinson and Ross, Stroudsburg, Pa.
Romo, G.E. and Linkswiler, H. (1969). Effect of level and pattern of essential amino acids on nitrogen retention of adult man. J. Nutr., 97, 147–153.
Rose, W.C. (1957). The amino acid requirements of adult man. Nutr. Abst. Rev., 27, 631–647.
WHO. Energy and Protein Requirements. (1973). Report of a Joint FAO/WHO Ad Hoc Expert Committee. WHO Tech. Rept. Series No. 522, World Health Organization, Geneva.
Young, V.R., Murray, E., Rand, W.M. and Scrimshaw, N.S. (1975). Protein requirements of man: comparative nitrogen balance response within the submaintenance-to-maintenance range of intakes of wheat and beef proteins. J. Nutr., 105, 534–542.
Young, V.R., Taylor, Y.S.M., Rand, W.M. and Scrimshaw, N.S. (1973). Protein requirements of man: efficiency of egg protein utilization at maintenance and submaintenance levels in young men. J. Nutr., 103, 1164–1174.
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Clark, H.E., Brewer, M.F., Bailey, L.B. (1978). Effect on Nitrogen Retention by Adults of Different Proportions of Indispensable Amino Acids in Isonitrogenous Cereal-Based Diets. In: Friedman, M. (eds) Nutritional Improvement of Food and Feed Proteins. Advances in Experimental Medicine and Biology, vol 105. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3366-1_5
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DOI: https://doi.org/10.1007/978-1-4684-3366-1_5
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