The NADPH Oxidase Activity of Polymorphonuclear Neutrophilic Leukocytes Revisited
The direct oxidation of NADPH by an activated NADPH oxidase was proposed several years ago by our laboratory (12,14,17) as the primary event of the metabolic stimulation in phagocytosing leukocytes that consists of increased oxygen uptake, increased production of hydrogen peroxide and superoxide anion, and increased activity of the hexose monophosphate shunt (1,3,4,5,6,15). The characteristics of the oxidase have been further investigated in our laboratory and the results of these studies are reported in the present paper.
KeywordsNADPH Oxidase NADPH Oxidase Activity NADPH Oxidation Guanidinium Chloride NADPH Concentration
Unable to display preview. Download preview PDF.
- 5.Johnston, R.B., Jr., Keele, B.B., Jr., Misra, H.P., Lehmeyer, M.J., Webb, S.L., Baehner, R.L. and Rajagopalan, K.V., J. Clin. Invest., 55 (1975) 1357.Google Scholar
- 7.Morita, Y. and Kameda, K., Mem. Res. Inst. Food Sci. Kyoto Univ., 23 (1961) 1.Google Scholar
- 8.Nicholls, P., Fed. Proc., 20 (1961) 50.Google Scholar
- 13.Rossi, F. and Zatti, M., Brit. J. Exp. PathoL, 45 (1964) 548.Google Scholar