Nitroblue Tetrazolium Reduction by Human Macrophages: Studies in Chronic Granulomatous Disease

  • J. L. Vildé
  • F. Vildé
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 73)


Properties and functions of macrophages have been extensively studied in animals, but little work has been done in humans because of the difficulties in obtaining a homogeneous and adequate mononuclear phagocyte population. Nitroblue tetrazolium (NBT) has been largely used with polymorphonuclear leukocytes (PMNL) to study the functions of phagocytes (12,16,21). This method can reveal some congenital defects of PMNL such as in chronic granulomatous disease (CGD) where intracellular killing of microorganisms is impaired (22).


Latex Particle Chronic Granulomatous Disease Mononuclear Phagocyte Human Macrophage Chronic Granulomatous Disease Patient 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • J. L. Vildé
    • 1
  • F. Vildé
    • 2
  1. 1.Hôpital Claude BernardUniversité Paris 7ParisFrance
  2. 2.Hôpital BoucicautUniversité Paris 5ParisFrance

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