Abstract
The oxygen saturation of myoglobin can be used as a quantitative indicator of the cellular oxygen supply3,11 as well as of the intracellular oxygen pressure12. On the hemoglobin free perfused Langendorff-heart preparation such measurements can be carried out non-invasively by reflection fotometry4,6,9,10,12 However, our investigations have shown, that under such conditions the usually applied two wavelength method may yield erroneous results 1) because of the superposition of the spectra of myoglobin and cytochromes and 2) the complicated optical conditions during reflection within the tissue4,5. Therefore a larger spectral range is monitored and a new multicomponent evaluation method applied which considers scattering using the two-flux theory of Kubelka and Munk (two-flux evaluation method for multicomponent systems: TEMMS5). The aim of the following experiments was to test the new method under relatively simple and reproducible conditions. These were achieved by arresting and cooling the heart. The results demonstrate quite good applicability of the new evaluation method.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
E. Antonini, Interrelationship between structure and function in hemoglobin and myoglobin, Physiol. Rev. 45:123–170 (1965).
R. Bünger, F. J. Haddy, and A. Querengässer, An isolated guinea pig heart preparation with in vivo like features, Pflügers Arch. 353:317–326 (1975).
H. Fabel, and D. W. Lübbers, Measurements of reflection spectra of the beating rabbit heart in situ, Biochem. Z. 341:351–356 (1965).
H. R. Figulla, J. Hoffmann, and D. W. Lübbers, Evaluation of reflection spectra of the isolated heart by multicomponent spectra analysis in comparison to other evaluation methods, in: “Oxygen Transport to Tissue V,” D. W. Lübbers, H. Acker, E. Leniger-Follert, and T.K. Goldstick, eds., Plenum Press, New York (1984), pp. 821–830.
J. Hoffmann, U. Heinrich, H. R. Ahmad, and D. W. Lübbers, Analysis of tissue reflection spectra obtained from brain or heart using the two flux theory from non-constant light scattering, in: “Oxygen Transport to Tissue VI,” D. F. Bruley et al., eds., Plenum Press, New York (1984), in press.
H. Kanaide, R. Yoshimura, N. Makino, and M. Nakamura, Regional myocardial function and metabolism during acute coronary artery occlusion, Am. J. Physiol. 242:H980–989 (1982).
M. Kessler, and D. W. Lübbers, Aufbau und Anwendungsmöglichkeit verschiedener Po2-Elektroden, Pflügers Arch. 291:R82 (1966).
D. W. Lübbers, and N. Niesei, Ein Kurzzeitspektralanalysator zur Registrierung schnell verlaufender Änderungen der Absorption, Naturwissenschaften 44:60 (1957).
D. W. Lübbers, H. H. Figulla, J. Hoffmann, and R. Wodick, The effect of hypoxic and histotoxic hypoxia on contractility and blood flow of the Langendorff guinea pig heart preparation, in: “Oxygen Transport to Tissie IV,” H. I. Bicher, and D. F. Bruley, eds., Plenum Press, New York (1983), pp. 225–230.
N. Makino, H. Kanaide, R. Yoshimura, and M. Nakamura, Myoglobin oxygenation remains constant during the cardiac cycle, Am. J. Physiol. 245:H237–243 (1983).
G. A. Millikan, Experiments on muscle haemoglobin in vivo: the instanteous measurement of muscle metabolism. Proc. Roy. Soc. 123:218–241 (1937).
M. Tamura, N. Oshino, B. Chance, and I. A. Silver, Optical measurements of intracellular oxygen concentration of rat heart in vitro. Arch. Biochem. 191:8–22 (1978).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Plenum Press, New York
About this chapter
Cite this chapter
Caspary, L., Hoffmann, J., Ahmad, H.R., Lübbers, D.W. (1985). Multicomponent Analysis of Reflection Spectra From The Guinea Pig Heart for Measuring Tissue Oxygenation By Quantitative Determination of Oxygen Saturation of Myoglobin and of The Redox State of Cytochrome aa3, c, and b. In: Kreuzer, F., Cain, S.M., Turek, Z., Goldstick, T.K. (eds) Oxygen Transport to Tissue VII. Advances in Experimental Medicine and Biology, vol 191. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3291-6_27
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3291-6_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3293-0
Online ISBN: 978-1-4684-3291-6
eBook Packages: Springer Book Archive