Abstract
The coupling of enzymes to polarographic and other electrochemical sensors has been a happy one (1–7). The first such union occurred in 1962 (8). The electrochemistry involved lends simplicity to the detecting equipment while in the sensor itself the enzyme provides the specificity, often a degree of specificity which cannot be obtained by any other means. In addition, and very importantly this type of sensor lends itself to miniaturization to the point where it may be used intracellularly.
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References
Clark, L. C., Jr, A polarographic enzyme electrode for the measurement of oxidase substrates. In: Oxygen Supply, edited by Manfred Kessler, et. al. Munchen-Berlin-Wien, Germany: Urban & Schwarzenberg, 120 - 128 (1973).
Clark, Leland C., Jr. A family of polarographic enzyme electrodes and the measurement of alcohol. Biotechnology & Bioengineering Symposium No. 3, 377 - 394 (1972).
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The enzyme electrode analytical instrument used in this research is a prototype. It is about to go into production as Model 23 and is available from the Yellow Springs Instrument Co., Yellow Springs, Ohio 45387, U.S.A.
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Clark, L.C., Clark, E.W. (1973). Differential Anodic Enzyme Polarography for the Measurement of Glucose. In: Bicher, H.I., Bruley, D.F. (eds) Oxygen Transport to Tissue. Advances in Experimental Medicine and Biology, vol 37 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3288-6_18
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DOI: https://doi.org/10.1007/978-1-4684-3288-6_18
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