Abstract
Difficulties of measuring oxygen saturation of circulating whole blood in microvessels have greatly limited past efforts to obtain such data. These difficulties are related to the small diameters of the vessels, the small, continuously changing volume of blood flowing in them, variations in the state of red cell orientation and suspension during flow, and numerous optical problems. In order to define some of the problems, establish specific relationships that may exist among the variables, and determine the sensitivity and reliability of our equipment and approach, we first studied certain important variables governing the measurement of oxygen saturation by transmitted light in small samples of whole blood in vitro where conditions can be more readily and completely controlled than in vivo. The studies were first done with static blood in thin layer cuvettes and then with blood in flow in small diameter polyethylene tubes.
Supported by grant HL-902 and HL-09447 from the National Heart and Lung Institute, National Institutes of Health, PHS, and Grant NGR 22-004-018 from the National Aeronautics and Space Administration.
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Berman, H.J., Segall, S.J., Fuhro, R.L. (1973). Dual Wavelength Micro-Oximeter of Hamster Whole Blood in Vitro. In: Bicher, H.I., Bruley, D.F. (eds) Oxygen Transport to Tissue. Advances in Experimental Medicine and Biology, vol 37 A. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3288-6_12
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DOI: https://doi.org/10.1007/978-1-4684-3288-6_12
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