Peroxydisulfate Anion-Induced Crosslinking of Proteins

  • Howard L. Needles
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 86A)


Peroxydisulfate anion in aqueous solutions is a strong oxidizing agent decomposing to sulfate free radicals and subsequently to hydroxyl radicals. in the presence of proteins, aqueous solutions of peroxydisulfate undergo more rapid induced decomposition to these free radical species, which in turn leads to oxidative attack and crosslinking and/or degradation of the protein. Under mild conditions and at low peroxydisulfate concentrations, protein crosslinking predominates over degradation. Peroxydisulfate-induced crosslinking of gelatin, fibroin, and other proteins is reviewed. The mechanism of crosslinking is interpreted in light of physical and chemical data, amino acid analyses, peroxydisulfate’s known mode of decomposition, the effect of protein modification on oxidative crosslinking, and related model compound studies.


Electron Spin Resonance Silk Fibroin Amino Acid Side Chain Oxidative Attack Cysteic Acid 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Howard L. Needles
    • 1
  1. 1.Division of Textiles and ClothingUniversity of CaliforniaDavisUSA

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