Skip to main content
SpringerLink
Log in

Effects of Neomycin on Polyphosphoinositides in Inner Ear Tissues and Monomolecular Films

  • Chapter
Membrane Toxicity

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 84))

Abstract

The effect of neomycin on polyphosphoinositides was studied in vivo and in vitro. In vivo, the incorporation of 32 Pi into phosphatidylinositoi phosphate and phosphatidylinositol diphosphate was measured in inner ear tissues. Concentrations of neomycin which decreased the electrophysiological response of the cochlea to sound stimulation also decreased labeling of phosphatidylinositol diphosphate. In vitro experiments with brain tissues and polyphosphoinositide extracts indicated a direct interaction between the lipids and neomycin.

Neomycin interacts strongly with monomolecular films of polyphosphoinositides. The interaction appears to be complex and is a function of neomycin concentration in the subphase and surface pressure of the film. Condensation of the polyphosphoinositide film is favored at low neomycin concentrations and low film pressures while expansion of the film is favored at high neomycin concentrations and high film pressures. The interactions of neomycin with other negatively charged films (phosphatidyl inositol and phosphatidyl serine) are much weaker, particularly at low neomycin concentrations.

The metabolic and physiological consequences of the neomycin/polyphosphoinositide interaction are discussed in regard to the ototoxicity of the drug.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. ABOOD, L. G. and HOSS, W.: European J. Pharmacol. 32 (1975) 66.

    Article  CAS  Google Scholar 

  2. ABRAMSON, M. B., KATZMAN, R. and GREGOR, H. P.: J. Biol. Chem. 239 (1964) 70.

    PubMed  CAS  Google Scholar 

  3. ALEXANDER, A. E., TEORELL, T. and ABORG, C. G.: Trans. Faraday Soc. 35 (1939) 1200.

    Google Scholar 

  4. ANDRE, T.: Acta Radiol. Suppl. 142 (1956) 1.

    PubMed  Google Scholar 

  5. BLAUSTEIN, M. P.: Biochim. Biophys. Acta 135 (1967) 653.

    Article  PubMed  CAS  Google Scholar 

  6. DEMEL, R. A., GUERTS VAN KESSEL, W. S. M., ZWAAL, R. F. A., ROELOFSEN, B. and VAN DEENEN, L. L. M.: Biochim. Biophys. Acta. 406 (1975) 97.

    Article  PubMed  CAS  Google Scholar 

  7. FEINSTEIN, M. B.: J. Gen. Physiol. 48 (1964) 357.

    Article  PubMed  CAS  Google Scholar 

  8. GOLDMAN, D. E.: Biophys. J. 4 (1964) 167.

    Article  CAS  Google Scholar 

  9. HAUSER, H. and DAWSON, R. M. C.: European J. Biochem. 1 (1967) 61.

    Article  CAS  Google Scholar 

  10. HAWKINS, J. E. Jr.: Biochemical aspects of ototoxicity, Ch. 24, Biochemical Mechanisms in Hearing and Deafness (Paparella, M.M., ed) Thomas. Springfield, IL (1970).

    Google Scholar 

  11. HENDRICKSON, H. S. and REINERTSEN, J. L.: Biochim. Biophys. Res. Comm. 44 (1971) 1258.

    Google Scholar 

  12. KAI, M. and HAWTHORNE, J. N.: Ann. N. Y. Acad. Sci. 165 (1969) 761.

    PubMed  CAS  Google Scholar 

  13. LODHI, S., WEINER, N. D. and SCHACHT, J.: Biochim. Biophys. Acta 426 (1976) 781.

    Article  PubMed  CAS  Google Scholar 

  14. MALICK, A. W., FELMEISTER, A. and WEINER, N. D.: J. Pharm. Sci. 62 (1973) 1871.

    Google Scholar 

  15. MALICK, A. W., WEINER, N. D. and FELMEISTER, A.: J. Pharm. Sci. 63 (1974) 398.

    Article  PubMed  CAS  Google Scholar 

  16. MICHELL, R. H.: Biochim. Biophys. Acta.415 (1975) 81.

    PubMed  CAS  Google Scholar 

  17. MULE, S. J.: Biochem. Pharmacol. 18 (1969) 339.

    Article  PubMed  CAS  Google Scholar 

  18. NUTTALL, A. L., MARQUES, D. M., STOCKHORST, E. and SCHACHT, J.: J. Acoust. Soc. Amer. 57 (1975) S60.

    Article  Google Scholar 

  19. ORSULAKOVA, A., STOCKHORST, E. and SCHACHT, J.: J. Neurochem. 26 (1976) 285.

    Article  PubMed  CAS  Google Scholar 

  20. PAPAHADJOPOULOS, D.: Biochim. Biophys. Acta 163 (1968) 240.

    Article  PubMed  CAS  Google Scholar 

  21. PAPAHADJOPOULOS, D.: Biochim. Biophys. Acta 241 (1971) 254.

    Article  PubMed  CAS  Google Scholar 

  22. PAPAHADJOPOULOS, D.: Biochim. Biophys. Acta 265 (1972) 169.

    PubMed  CAS  Google Scholar 

  23. PESTKA, S.: Ann. Rev. Biochem. 40 (1971) 697.

    Article  Google Scholar 

  24. PITTINGER, C. and ADAMSON, R.: Ann. Rev. Pharmacol. 12 (1972) 169.

    Article  PubMed  CAS  Google Scholar 

  25. ROJAS, E. and TOBIAS, J. M.: Biochim. Biophys. Acta 94 (1965) 394.

    Article  PubMed  CAS  Google Scholar 

  26. SANTIAGO-CALVO, E., MULE, S., REDMAN, C. M., HOKIN, M. R., and HOKIN, L. E.: Biochim. Biophys. Acta 84 (1964) 550.

    PubMed  CAS  Google Scholar 

  27. SCHACHT, J. and AGRANOFF, B. W.: J. Biol. Chem. 247 (1972) 771.

    PubMed  CAS  Google Scholar 

  28. SCHACHT, J., LODHI, S., and WEINER, N. D.: Trans. Amer. Soc. Neurochem. 7 (1976) 126.

    Google Scholar 

  29. SCHULMAN, J. H., and HUGHES, A. H.: Biochem. J. 29 (1935) 1236.

    Google Scholar 

  30. SCHULMAN, J. H. and FRIEND, JR., J. A.: J. Soc. Cosmetic Chem. 1 (1949) 381.

    Google Scholar 

  31. SEEMAN, P.: Pharmacol. Rev. 24 (1972) 583.

    PubMed  CAS  Google Scholar 

  32. SHAH, D. O. and SCHULMAN, J. H.: J. Lipid Res. 6 (1965) 341.

    PubMed  CAS  Google Scholar 

  33. WERSALL, J. and FLOCK, A.: Life Sci. 3 (1964) 1151.

    Article  Google Scholar 

  34. WHITE, G. L., SCHELLHASE, H. U., and HAWTHORNE, J. N.: J. Neurochem, 22 (1974) 149.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kresge Hearing Research Institute, University of Michigan, Ann Arbor, Michigan, 48109, USA

    J. Schacht

  2. College of Pharmacy, University of Michigan, Ann Arbor, Michigan, 48109, USA

    S. Lodhi & N. D. Weiner

Authors
  1. J. Schacht
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Lodhi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. D. Weiner
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The University of Rochester, Rochester, New York, USA

    Morton W. Miller  & Adil E. Shamoo  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Plenum Press, New York

About this chapter

Cite this chapter

Schacht, J., Lodhi, S., Weiner, N.D. (1977). Effects of Neomycin on Polyphosphoinositides in Inner Ear Tissues and Monomolecular Films. In: Miller, M.W., Shamoo, A.E. (eds) Membrane Toxicity. Advances in Experimental Medicine and Biology, vol 84. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3279-4_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-3279-4_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3281-7

  • Online ISBN: 978-1-4684-3279-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation