Abstract
Turtle bladders actively transport Na, Cl, and HCO3 to the serosal fluid; and each ionic flux is independent of the others under short-circuiting conditions. This behavior mimics that of a parallel network of ion-selective, electrically-conductive paths and pumps in each membrane, — a picture consistent with recent evidence along three independent lines. (1) The potential response to increases in mucosal Na concentration indicates that the Na conductance of the apical membrane is 70% of the transepithelial conductance and that the Na transfer across this membrane occurs via an electrically-charged carrier operation. (2) The sidedness and selectivity of transport changes induced by certain agents are the following. Acting from the mucosal side only, amiloride blocks passive Na transfer; and catecholamines (or imidazoles or theophylline) accelerate active anion transport. Acting from the serosal side only, ouabain blocks active Na transport; and disulfonic stilbenes or acetazolamide block passive anion transfers. (3) The surface charge density of the apical membrane differs from that of basal-lateral during free-flow electrophoresis (FFE) of a mixed membrane fraction of epithelial cells. Basal-lateral membrane fragments (containing ouabain-sensitive ATPase and a stilbene-binding protein) migrate toward the positive electrode while apical membrane fragments (contain nor-epinephrine-sensitive adenylate cyclase and cAMP-activated protein kinase) migrate toward the negative electrode. (4) Thus, ouabain, nor-epinephrine, and a disulfonic stilbene are shown to be useful membrane probes for the Na pump, the anion pumps, and the passive anion transfer paths, respectively.
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© 1977 Plenum Press, New York
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Brodsky, W.A., Ehrenspeck, G. (1977). The Localization of Ion-Selective Pumps and Paths in the Plasma Membranes of Turtle Bladders. In: Miller, M.W., Shamoo, A.E. (eds) Membrane Toxicity. Advances in Experimental Medicine and Biology, vol 84. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3279-4_3
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DOI: https://doi.org/10.1007/978-1-4684-3279-4_3
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