Abstract
Chinese hamster ovary (CHO) cells previously selected in a single-step for resistance to one or two different lectins and assigned to individual phenotyp-ic groups on the basis of their unique patterns of lectin resistance, have been examined for their lectin-binding abilities. The lectin-binding parameters of CHO cells were shown to be very complex in a detailed study of the binding of 125I-WGA to wild-type (WT) cells. On the basis of these results, standard assay conditions were established and comparative binding studies between the twenty-two WT and lectin-resistant (LecR) clones were performed. A general correlation of lectin resistance with decreased lectin-binding ability and of lectin sensitivity with increased lectinbinding ability was found, although several exceptions to this trend were observed.
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Stanley, P., Carver, J.P. (1977). Lectin Receptors and Lectin Resistance in Chinese Hamster Ovary Cells. In: Miller, M.W., Shamoo, A.E. (eds) Membrane Toxicity. Advances in Experimental Medicine and Biology, vol 84. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3279-4_13
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DOI: https://doi.org/10.1007/978-1-4684-3279-4_13
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