3-D-(−) Hydroxybutyrate Dehydrogenase from Rat Liver Mitochondria — Purification and Interaction with Phospholipids

Vidal, J. C.; Guglielmucci, E. A.; Stoppani, A. O. M.

doi:10.1007/978-1-4684-3276-3_18

3-D-(−) Hydroxybutyrate Dehydrogenase from Rat Liver Mitochondria — Purification and Interaction with Phospholipids

J. C. Vidal³,
E. A. Guglielmucci³ &
A. O. M. Stoppani³

Chapter

119 Accesses
5 Citations

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 83))

Abstract

The existence of membrane-bound enzymes has been known for many years and it has been proposed that most particulate enzymes are associated with the lipid matrix of biological membranes. Fleischer & Klouwen (1961) were the first to show the lipid requirement in enzyme reactions of the mitochondrial electron transport system. It is now recognized that there are a number of membrane-bound enzymes which require phospholipid for function. Any lipid-requiring enzyme must fulfill two minimal experimental criteria for proof of lipid involvement, namely (a) removal of lipid by mild procedures result in loss of enzymic activity, and (b) the activity is restored when lipids are added in a suitable physical state to the delipidized enzymes. Point (b) entails the demonstration that activity is restored after rebinding of phospholipid to the enzyme.

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Authors and Affiliations

Facultad de Medicina, Instituto de Química Biológica, Paraguay 2155, 1121-C.F., Buenos Aires, Argentina
J. C. Vidal, E. A. Guglielmucci & A. O. M. Stoppani

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J. C. Vidal
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E. A. Guglielmucci
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A. O. M. Stoppani
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Universidad Nacional del Sur — CONICET, Bahía Blanca, Argentina
Nicolás G. Bazán & Norma M. Giusto &
Universidad Nacional de La Plata, La Plata, Argentina
Rodolfo R. Brenner

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Vidal, J.C., Guglielmucci, E.A., Stoppani, A.O.M. (1977). 3-D-(−) Hydroxybutyrate Dehydrogenase from Rat Liver Mitochondria — Purification and Interaction with Phospholipids. In: Bazán, N.G., Brenner, R.R., Giusto, N.M. (eds) Function and Biosynthesis of Lipids. Advances in Experimental Medicine and Biology, vol 83. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3276-3_18

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DOI: https://doi.org/10.1007/978-1-4684-3276-3_18
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Online ISBN: 978-1-4684-3276-3
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