Abstract
The plasma very low density lipoproteins (VLDL) are the major vehicle for the transport in human blood of endogenously synthesized triglycerides. VLDL particles contain about 50% triglyceride, 20% cholesterol, 20% phospholipid, and 10% protein, by weight (1). The plasma of subjects with Type IV or V hyperlipoproteinemia (2) is rich in VLDL and is a convenient source of these lipoproteins. After the delipidation of VLDL with mixed organic solvents such as chloroform-methanol (2:1) or diethyl ether-ethanol (3:1), the resulting lipid-free apoproteins can be separated on Sephadex G-150 into two major fractions (3). The protein(s) that elutes at the void volume is identical in many respects to the major apoprotein(s) of LDL (4). The other fraction, which is not excluded from the gel, contains a mixture of several apoproteins which can be fractionated on DEAE-cellulose (Figure 1) and have been referred to collectively as the C-proteins (5), the D-peptides (6) or Fraction V (7a). One of these apoproteins, apoLP-Ala (apoC-III), has been studied extensively. It exists as a linear polypeptide containing 79 amino acids (Figure 2) and has a calculated molecular weight of 8751, excluding carbohydrate (8, 9).
Established Investigators of the American Heart Association.
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Abbreviations
- ApoLp-Ala:
-
apolipoprotein-alanine or apoC-III, the most abundant of the human C-proteins
- VLDL:
-
very low density lipoproteins, normally isolated at d < 1.006 g/ml
- LDL:
-
low density lipoproteins, normally isolated at d 1.006–1.063 g/ml
- PC:
-
phosphatidylcholine.
References
Skipski, J. P. In: Blood Lipids and Lipoproteins: Quantitation, Composition, and Metabolism. G. J. Nelson, editor. Wiley-Interscience, New York, 1972, p. 471.
Fredrickson, D. S. and Levy, R. I. In: The Metabolic Basis of Inherited Diseases.Third Edition. J. B. Stanbury, J. B. Wyngaarden, D. S. Fredrickson, editors. McGraw-Hill, New York, 1972, p. 545.
Brown, W. V., Levy, R. I. and Fredrickson, D. S. J. Biol. Chem. 244: 5687, 1969.
Gotto, A. M., Brown, W. V., Levy, R. I., Birnbaumer, M. E., and Fredrickson, D.S. J. Clin. Invest. 51: 1486, 1972.
Alaupovic, P. Atherosclerosis 13:141, 1971.
Brown, W. V., Levy, R. I. and Fredrickson, D. S. J. Biol. Chem. 245: 6588, 1970.
Scanu, A., Toth, J., Edelstein, C., Koga, S. and Stiller, E. Biochemistry 8: 3309, 1969.
Albers, J. J. and Scanu, A. M. Biochim. Biophys. Acta 236: 29, 1971.
Shulman, R. S., Herbert, P. N., Fredrickson, D.S., Wehrly, K., and Brewer, H. B. J. Biol. Chem. 249:4969, 1974.
Brewer, H. B., Shulman, R., Herbert, P., Ronan, R. and Wehrly, K. J.Biol. Chem. 249:4975, 1974.
Morrisett, J. D., David, J. S. K., Pownall, H. J., and Gotto, A. M. Biochemistry 12: 1290, 1973.
Pownall, H. J. and Smith, L. C. Biochemistry 13: 2590, 1974.
Hoff, H. F., Morrisett, J. D. and Gotto, A. M. Biochim. Biophys. Acta 296: 653, 1973.
Howell, J. I., Lucy, J. A., Pirola, R. C. and Bouchier, I. A. D. Biochim. Biophys. Acta 210: 1, 1970.
Hinz, H-J and Sturtevant, J. M. J. Biol. Chem. 247:6071, 1972.
Shimshick, E. J. and McConnell, H. M. Biochemistry 12: 2351, 1973.
Novosad, Z. F., Pownall, H. P., Gotto, A. M. and Morrisett, J. D. In preparation.
Birks, J. B. Photophysics of Aromatic Molecules. Wiley-Interscience, London, 1970, Chapter 10.
Pownall, H. J., Morrisett, J. D., Sparrow, J. T., and Gotto, A. M. Biochem. Biophys. Res. Commun. 60: 779, 1974.
Pownall, H. J., Morrisett, J. D., Sparrow, J. T., and Gotto, A. M. Biochem. Biophys. Res. Commun. 60: 779, 1974.
Tsong, T. Y. Proc. Nat. Acad. Sci. (USA) 71: 2684, 1974.
Pownall, H. J., Morrisett, J. D., Sparrow, J. T., and Gotto, A. M. Submitted for publication.
Phillips, M. C., Finer, E.G., and Hauser, H. Biochim. Biophys. Acta 290: 397, 1972.
Huang, C. Biochemistry 8: 344, 1969.
Morrisett, J. D. and Gotto, A. M. In preparation.
Morrisett, J. D., Gallagher, J. G., Aune, K.C., and Gotto, A. M. Biochemistry 13: 4765, 1974.
Segrest, J. P., Jackson, R. L., Morrisett, J. D. and Gotto, A. M. FEBS Letters 38: 247, 1974.
Merrifield, R. B. Adv. Enzy. 32:221, 1969.
Sparrow, J. T., Gotto, A. M. and Morrisett, J. D. Proc. Nat. Acad. Sci. (USA) 70: 2124, 1973.
Sparrow, J T., Gotto, A. M. and Morrisett, J. D. Unpublished results.
Baker, H. N., Delahunty, T., Gotto, A. M. and Jackson, R. L. Proc. Nat. Acad. Sci. (USA) 71: 3631, 1974.
Brewer, H. B., Lux, S. E., Ronan, R., and John, K. M. Proc. Nat. Acad. Sci. (USA) 69: 1304, 1972.
Shulman, R., Herbert, P., Wehrly, K., Chesebro, B., Levy, R. I., and Fredrickson, D. S. Circulation 45: II: 246.
Jackson, R. L., Sparrow, J. T., Baker, H. N., Morrisett, J. D., Taunton, O. D., and Gotto, A. M. J. Biol. Chem. 249: 5308, 1974.
Morrisett, J. D., Jackson, R. L. and Gotto, A. M. Ann. Rev. Biochem., in press.
Jackson, R. L., Morrisett, J. D., Gotto, A. M., and Segrest, J. P. Mol. Cell. Biochem., in press.
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Morrisett, J.D., Pownall, H.J., Sparrow, J.T., Jackson, R.L., Gotto, A.M. (1975). The Interaction of Apolipoprotein-Alanine (ApoC-III) with Lipids: Study of Structural Features Required for Binding. In: Kritchevsky, D., Paoletti, R., Holmes, W.L. (eds) Lipids, Lipoproteins, and Drugs. Advances in Experimental Medicine and Biology, vol 63. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3258-9_2
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