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Theory of Radioimmunoassays and Hormone-Receptor Interactions:

II. Simulation of Antibody Divalency, Cooperativity and Allosteric Effects

  • Chapter
Receptors for Reproductive Hormones

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 36))

Abstract

Since antibody and some hormone receptor molecules are known to possess two binding sites, it is possible that the intrinsic affinity constant for the “second site”, after one site is filled, is greater than the intrinsic affinity constant for the first site; this would result in “cooperative” or apparent “allosteric” effects. The mathematical theory of “cooperative” binding systems, both at equilibrium and in the transient state, has been applied to radioimmunoassay systems. The effects of affinity constants, antibody concentration, and “tracer” concentration, have been examined in several coordinate systems. Further, the kinetics of these reaction systems have been simulated. This theory should be useful in design and analysis of experiments to test for the presence of “cooperativity” effects.

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Author information

Authors and Affiliations

  1. Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20015, USA

    D. Rodbard

  2. Kalamazoo College, Kalamazoo, Michigan, USA

    R. E. Bertino

Authors
  1. D. Rodbard
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  2. R. E. Bertino
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Editor information

Editors and Affiliations

  1. Department of Cell Biology, Baylor College of Medicine, Houston, Texas, USA

    Bert W. O’Malley M.D.  & Anthony R. Means Ph.D.  & 

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© 1973 Plenum Press, New York

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Rodbard, D., Bertino, R.E. (1973). Theory of Radioimmunoassays and Hormone-Receptor Interactions:. In: O’Malley, B.W., Means, A.R. (eds) Receptors for Reproductive Hormones. Advances in Experimental Medicine and Biology, vol 36. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3237-4_15

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  • DOI: https://doi.org/10.1007/978-1-4684-3237-4_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-3239-8

  • Online ISBN: 978-1-4684-3237-4

  • eBook Packages: Springer Book Archive

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