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Measurement of Platelet Aggregation and Shape Change

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Platelet Function and Thrombosis

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 34))

Abstract

Platelet aggregation and shape change are possibly the only platelet functions which can be measured in vitro with a degree of reliability and validity. The nephelometric method of Born (3) has proven to be one of the most valuable tools in platelet research. The method takes advantage of the changes in the optical density of platelet—rich plasma (PRP), or platelets suspended in an appropriate “plasma-free” medium, when a substance causing them to clump is added. Changes in light transmission which, within limits, are directly proprotional to the concentration of the aggregating agent, can be recorded photometrically. When the temperature of the system and the collision forces between platelets are controlled (6), it is possible to measure platelet aggregation in a highly quantitative manner (4, 9).

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References

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Author information

Authors and Affiliations

  1. M.R.C. Thrombosis Research Group, Dept. of Pharmacology, Royal College of Surgeons, Lincoln’s Inn Fields, London, W.C.2, UK

    F. Michal

Authors
  1. F. Michal
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Editor information

Editors and Affiliations

  1. Hemophilia and Thrombosis Center, University of Milan, Milan, Italy

    P. M. Mannucci  & S. Gorini  & 

  2. Policlinico Hospital, Milan, Italy

    P. M. Mannucci  & S. Gorini  & 

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© 1972 Plenum Press, New York

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Michal, F. (1972). Measurement of Platelet Aggregation and Shape Change. In: Mannucci, P.M., Gorini, S. (eds) Platelet Function and Thrombosis. Advances in Experimental Medicine and Biology, vol 34. Springer, New York, NY. https://doi.org/10.1007/978-1-4684-3231-2_15

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  • DOI: https://doi.org/10.1007/978-1-4684-3231-2_15

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4684-3233-6

  • Online ISBN: 978-1-4684-3231-2

  • eBook Packages: Springer Book Archive

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