Abstract
This paper gives an account of our experiences with the substrate ratio technique for calculating cytoplasmic and mitochondrial NAD+/NADH2 changes in brain and liver in several metabolic states. The NAD redox system is especially suitable for studies on oxygen availability since it occurs in both the cytoplasm and the mitochondria; this makes it possible to distinguish changes in the NAD+/NADH2 ratio in the two compartments. NAD exists in cells in both free and bound forms. Krebs (1967) and Krebs and Veech (1969) recommend that, on thermodynamic grounds, only the free nucleotide concentrations should be used in calculating redox ratios.
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© 1973 Plenum Press, New York
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Miller, A.T., Lai, F.M.H. (1973). Brain and Liver Intracellular Compartmental Redox States in Hypoxia, Hypocapnia and Hypercapnia. In: Kovách, A.G.B., Stoner, H.B., Spitzer, J.J. (eds) Neurohumoral and Metabolic Aspects of Injury. Advances in Experimental Medicine and Biology, vol 33. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3228-2_35
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DOI: https://doi.org/10.1007/978-1-4684-3228-2_35
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