Studies on the Ability of Stored Blood to Transport Oxygen In Vivo
- 141 Downloads
Blood stored in acid-citrate-dextrose (ACD) shows a progressive increase in oxygen affinity primarily as the result of a decrease in red cell 2,3-diphosphoglycerate (DPG)1. Restoration of DPG levels (and presumably normal blood oxygen affinity) appears to occur promptly after transfusion of ACD-stored, DPG- depleted blood. Delayed restoration of DPG and oxygen affinity may impair oxygen delivery. Beutler and Wood reported that DPG values returned to nearly 50 percent of normal within 4 hours.2 Others have indicated that at least 25 percent of the final DPG level was achieved within 8 hours after completion of transfusion.3 Whether the rate of DPG restoration is significantly variable from patient to patient is uncertain because only a few patients have been reported.
KeywordsOxygen Tension Exchange Transfusion Fresh Blood Oxygen Affinity Arterial Oxygen Tension
Unable to display preview. Download preview PDF.
- 2.Beutler, E., and Wood, L.: The in vivo regeneration of red cell 2,3 diphosphoglyceric acid (DPG) after transfusion of stored blood. J. Lab. & Clin. Med. 74: 300, 1969.Google Scholar
- 3.Valeri, C. R. and Hirsch, M. M.: Restoration in vivo of erythrocyte adenosine triphosphate, 2,3-diphosphoglycerate, potassium ion, and sodium ion concentrations following the transfusion of acid-citrate-dextrose-stored human red blood cells. J. Lab. & Clin. Med. 73: 722, 1969.Google Scholar
- 4.Valeri, C. R. and Collins, F. B.: Physiologic effects of 2,3-DPG-depleted red cells with high affinity for oxygen. J. of Applied Physiology. 31: 823, 1971.Google Scholar
- 5.Sugerman, H. J., Davidson, D. T., Vibul, S.,Delivoria-Papadopoulos, M., Miller, L. D., and Oski, R. A.: The basis of defective oxygen delivery from stored blood. Surg., Gyne. & Obst., 131: 733, 1970.Google Scholar
- 6.Aster, R. H.: Studies of the mechanism of flhypersplenicff thrombocytopenia in rats. J. Lab. & Clin. Med. 70: 736, 1967.Google Scholar
- 9.Bromberg, P. A.: A spectrophotometric technique for measurement of blood oxygen dissociation curves on small samples. (To be published).Google Scholar
- 10.Van Liew, H. D.: Tissue PO2 and PCO2 estimation with rat subcutaneous gas pockets. J. Appl. Physiol. 17: 851, 1962.Google Scholar