Effects of Cyanate in Rabbits
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Cerami and Manning have proposed that cyanate be used in the treatment of persons with sickle cell disease (1). These investigators have shown that this compound inhibits sickling in vitro (1); that cells treated with cyanate in vitro have a prolonged survival in vivo when reinfused into the donor (2); and that cyanate has no adverse effects upon red cell metabolism (3). Although it was first reported that sickle cells treated with cyanate were rendered incapable of sickling, it has now been shown that these cells do sickle but lower oxygen tensions are required (4,5). Cyanate reacts with hemoglobin primarily by carbamylation of the α-amino group of both the α and β chains (6). The carbamylated hemoglobin has an increased affinity for oxygen (6) probably resulting from its inability to react with DPG (7) which is normally bound by the N-terminal amino groups of the β chain (8).
KeywordsSickle Cell Abnormal Hemoglobin Oxygen Dissociation Curve Hemoglobin Electrophoresis Oxyhemoglobin Dissociation Curve
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