Abstract
The purpose of the first clinical study with cyanate (Gillette, et al., 1971) was to determine whether the anti-sickling effect observed in vitro (Cerami and Manning, 1971) would also occur in vivo. Since the life span of erythrocytes from patients with sickle cell disease is markedly shortened, survival of these cells should be prolonged if their tendency to sickle were decreased. To test this hypothesis, chromium-51 (51Cr) half- times were determined with untreated autologous cells by the technique of Gray and Sterling (1950) modified by washing with saline. At a later time the survival of an aliquot of cells treated in vitro with cyanate was determined. Reaction of the cells in vitro with chromate and cyanate was shown to be non-competitive, and in other studies the elution rate of chromium was not altered by carbamylation. Double labelled survival studies in mice, rats, dogs and monkeys have also shown that carbamylation does not alter elution.
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Cerami, Anthony and Manning, James M. (1971). Potassium cyanate as an inhibitor of the sickling of erythrocytes in vitro. Proc. Nat. Acad. Sci. USA 68: 1180.
Gillette, Peter N., Manning, James M., and Cerami, Anthony (1971). Increased survival of sickle-cell erythrocytes after treatment in vitro with sodium cyanate. Proc. Nat. Acad. Sci. USA 68: 2791.
Gray, S. J., and Sterling, H. (1950). The tagging of red cells and plasma proteins with radioactive chromium. J. Clin. Invest. 29: 1604.
Graziano, J. H., Allen, T. A., and Cerami, A. (1972). The effect of cyanate on the endocrine system. Federation Proc. 31: 271. Abstract.
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© 1972 Plenum Press, New York
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Gillette, P.N., Peterson, C.M., Manning, J.M., Cerami, A. (1972). Preliminary Clinical Trials with Cyanate. In: Brewer, G.J. (eds) Hemoglobin and Red Cell Structure and Function. Advances in Experimental Medicine and Biology, vol 28. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3222-0_22
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DOI: https://doi.org/10.1007/978-1-4684-3222-0_22
Publisher Name: Springer, Boston, MA
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