Effect of Chlorthalidone Binding on the Electrophoretic Properties of Human Red Cell Carbonic Anhydrase Isozymes

  • Richard E. Tashian
  • Ya-Shiou L. Yu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 28)


The manner in which aromatic sulfonamide inhibitors bind to the carbonic anhydrases can provide us with useful information concerning the mechanisms of carbonic anhydrase action. Although considerable data are now available on carbonic anhydrase-sulfonamide binding kinetics (cf. Maren, 1967; Lindskog et al,, 1972), much less is known about structural changes in carbonic anhydrase brought about by sulfonamide binding. Recently, spectral analyses by King and Burgen (1970) and King and Roberts (1971) of sulfonamide complexes with human carbonic anhydrases, indicate that conformational changes occur in the enzymes as a result of the sulfonamide binding. In the present brief communication, we report some preliminary findings which may provide additional evidence for sulfonamide-induced conformational changes in carbonic anhydrase molecules.


Carbonic Anhydrase Esterase Activity Electrophoretic Pattern Carbonic Anhydrase Action Human Carbonic Anhydrases 
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  1. King, R.W., and Burgen, A.S.V., Biochem. Biophys. Acta, 207, 278 (1970).PubMedGoogle Scholar
  2. King, R.W., and Roberts, G.C.K., Biochemistry, 10, 558 (1971).PubMedCrossRefGoogle Scholar
  3. Lindskog, S., Henderson, L., Kannan, K.K., Liljas, A., Nyrnan, P. O., and Strandberg, B., in P.D. Boyer (Editor), The enzymes, Vol. 5, Academic Press, New York, 1971, p. 587.Google Scholar
  4. Maren, T.M., Physiol. Rev., 47, 595 (1967).PubMedGoogle Scholar
  5. Tanis, R.J., and Tashian, R.E., Biochemistry, 10, 4852 (1971).PubMedCrossRefGoogle Scholar
  6. Tanis, R.J., Tashian, R.E., and Yu, Y.-S.L., J. Biol. Chem., 245, 6003 (1970).PubMedGoogle Scholar
  7. Tashian, R.E., in J.J. Yunis (Editor), Biochemical methods in red cell genetics, Academic Press, New York, 1969, p. 307.Google Scholar
  8. Tashian, R.E., Compt. Rend. Trav. Lab. Carlsberg, 37, 359 (1970).Google Scholar
  9. Warra, I., Lövgren, S., Liljas, A., Kannan, K.K., and Bergsten, P.C., 1972, in Proceedings of this Conference.Google Scholar

Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • Richard E. Tashian
    • 1
  • Ya-Shiou L. Yu
    • 1
  1. 1.Department of Human GeneticsUniversity of Michigan Medical SchoolAnn ArborUSA

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