Neuropathology of Glycopeptides Derived from Brain Glycoproteins

  • E. G. Brunngraber
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 25)


In another chapter in this volume we described the current status of research on the heteropolysaccharide chains of neural glycoproteins. We have described a procedure (1) in which it is possible to isolate and determine gangliosides, glycoproteins, and glycosaminoglycans in brain tissue samples. Gangliosides are extracted with chloroform-methanol (2:1 and 1:2, v/v). The defatted tissue is treated with papain or pronase to liberate glycopeptides (derived from brain glycoproteins) and glycosaminoglycans. The solubilized glycopeptides are separated into nondialyzable and dialyzable glycopeptide fractions. Glycosaminoglycans are separated from the nondialyzable glycopeptides by precipitation with cetylpyridinium chloride. Application of the method to normal samples provided a clearcut separation of glycopeptides, glycosaminoglycans, and gangliosides.


White Matter Gray Matter Keratan Sulfate Carbohydrate Composition Mannose Residue 
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Copyright information

© Plenum Press, New York 1972

Authors and Affiliations

  • E. G. Brunngraber
    • 1
  1. 1.Illinois State Psychiatric InstituteChicagoUSA

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