In Vitro Studies of Delayed Hypersensitivity by the Method of Macrophage Spreading Inhibition.Delayed Hypersensitivity to Tuberculin and Diphtheria Toxoid in Rats
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It was shown by early experiments (1) in mice or guinea pigs having delayed hypersensitivity, that the ability of peritoneal macrophages to spread is markedly decreased by the presence of specific antigen. The spreading inhibition can be easily determined and used as a simple in vitro test for the study of delayed hypersensitivity. The method was named macrophage spreading inhibition (MSI) and is usefully applied for the detection of delayed hypersensitivity to bacterial and protozoal antigens in guinea pigs and mice (1,2,3,4).
KeywordsPeritoneal Macrophage Intradermal Injection Cutaneous Reactivity Peritoneal Cell Diphtheria Toxoid
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