Light and Electron Microscopic Changes in Lymph Nodes after Local Application of Heterologous Antithymocyte and Anti-Macrophage Serum

  • H. K. Müller-Hermelink
  • A. Thiede
  • H.-G. Sonntag
  • W. Müller-Ruchholtz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 15)


Two morphologically different cell types are involved in the production of immunological sensitization against many antigens, namely, macrophages and lymphocytes (e.g., 4, 15, 22, 3). Applying antisera specifically directed against either of these cells may result in a delay or suppression of the primary immunological response. This has been proved to be true for antithymocyte sera (ATS) (e.g., 23, 8, 14, 1). Also, studies on the in vivo action of antimacrophage sera (AMS) revealed prolongation of allogeneic skin graft survival time in rats (17). The present work intends to elucidate morphologically whether or not the apparently similar effect of these antisera (prolongation of skin graft survival time) is caused by a specific action on different target cells (lymphocytes and macrophages) in vivo. Therefore, we studied the morphological alterations in popliteal lymph nodes following local application of either antiserum. The electron microscopic observations presented here are added to recently published light microscopic and enzyme histochemical studies (19, 20).


Mast Cell Popliteal Lymph Node Immunosuppressive Capacity Antilymphocyte Serum Acid Phosphatase Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Barth, R. A., and J. Southworth Studies on heterologous antithymocyte sera. II. Effect on cellular antibody production during the early primary and secondary immune response of mice to sheep erythrocytes. J. Immunol., 101:1283, 1968.PubMedGoogle Scholar
  2. 2.
    Fiore-Donati, L., G. M. Cappuzzo, D. Collavo, N. Penelli, and L. Chieco-Bianchi. Morphological changes in lymphoid tissues of mice treated with antilymphocyte sera. In: Fiore-Donati, L., and M. G. Hanna, Jr. (eds.), Lymphatic tissue and germinal centers in immune response. Plenum Press, New York, p. 343, 1969.CrossRefGoogle Scholar
  3. 3.
    Fishman, M. Induction of antibodies in vitro. Ann. Rev. Microbiol. 23:199, 1969.CrossRefGoogle Scholar
  4. 4.
    Gallily, R., and M. Feldman. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology 12:197, 1967.PubMedGoogle Scholar
  5. 5.
    Gräsbeck, R., C. Nordman, and A. De la Chapelle. Mitogenic action of antileukocyte immune serum on peripheral leukocytes in vitro. Lancet II:385, 1963.CrossRefGoogle Scholar
  6. 6.
    Grogan, J. B. Alterations in phagocytic function of rats after treatment with antilymphocyte serum. RES J. Reticuloendoth. Soc., 6:411, 1969.Google Scholar
  7. 7.
    Hirsch, M. S., G. W. Gram Jr., and F. A. Murphy. In vitro and in vivo properties of antimacrophage sera. J. Immunol., 102:656, 1968.Google Scholar
  8. 8.
    James, K. Anti-lymphocytic antibody. A review. Clin. exp. Immunol., 2:615, 1967.Google Scholar
  9. 9.
    Jennings, J. F., and L. A. Hughes. Inhibition of phagocytosis by antimacrophage antibodies. Nature 221:79, 1969.PubMedCrossRefGoogle Scholar
  10. 10.
    Karnovsky, M. J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol., 27:137A, 1965.Google Scholar
  11. 11.
    Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. I. Evidence for opsonization. J. Immunol., 103:979, 1969.PubMedGoogle Scholar
  12. 12.
    Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. II. Nature and specificity of opsonizing antibody. J. Immunol., 103:990, 1969.PubMedGoogle Scholar
  13. 13.
    Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. III. Opsonizing activity of antihuman lymphocyte serum. J. Immunol., 103:1000, 1969.PubMedGoogle Scholar
  14. 14.
    Medawar, P. B. Biological effects of heterologous antilymphocyte sera. In: Human transplantation. F. D. Rapaport and J. D. Dausset (eds.), New York and London, pp. 501, Grune and Stratton, 1968.Google Scholar
  15. 15.
    Mosier, D. E. A requirement for two cell types in antibody formation in vitro. Sci., 158:1573, 1967.CrossRefGoogle Scholar
  16. 16.
    Riethmüller, G., D. Riethmüller, P. Rieber, and H. Stein. In vitro stimulation of lymphoid cells by antilymphocytic antibody. Bayer Symposium, Problems in Immunology, Springer, Berlin-Heidelberg-New York, 1969.Google Scholar
  17. 17.
    Sonntag, H.-G., and W. Mliller-Ruchholtz. Über den Einfluss auf die Überlebensdauer allogenetischer Rattenhaut-Transplantate. Vortag 2. Arbeitstag. Dtsch. Ges. Hyg. Mikrobiol., Mainz 1968.Google Scholar
  18. Sonntag, H.-G., and W. Mliller-Ruchholtz. Autoref. Zbl. Bakt., I. Ref. 215:515, 1969.Google Scholar
  19. 18.
    Taub, R. N., and E. M. Lance. Histopathological effect in mice of heterologous antilymphocyte serum. J. Exper. Med., 128:1281, 1968.CrossRefGoogle Scholar
  20. 19.
    Thiede, A., H.-G. Sonntag, and W. Müller-Ruchholtz. Unterschiedliche morphologische Veränderungen an Lymphknoten nach lokaler Applikation Heterologer Antithymocyten-und Antimakrophagen Seren. Virchows Arch., Abt. B, 3:302, 1969.Google Scholar
  21. 20.
    Thiede, A., H.-G. Sonntag, L.-D. Leder, H.-K. Müller-Hermelink, and W. Müller-Ruchholtz. Lymphknotenveränderungen nach lokaler Injektion heterologer antithymocyten und antimakrophagen seren. Verh. Dtsch. Ges. Path., 54, 1970.Google Scholar
  22. 21.
    Tridente, D., and D. W. Van Bekkum. Effect of antilymphocyteserum on mouse lymphoid tissues in vivo and in vitro. In: Fiore-Donati, L., and M. G. Hanna, Jr., (eds.), Lymphatic tissue and germinal centers in immune response. Plenum Press, New York, p. 371, 1969.CrossRefGoogle Scholar
  23. 22.
    Unanue, E. R. Properties and some uses of antimacrophage antibodies. Nature, 218:36, 1969.CrossRefGoogle Scholar
  24. 23.
    Woodruff, M. F. A., and N. F. Anderson. The effect of lymphocyte depletion by thoracic duct fistula and the administration of antilymphocytic serum on the survival of skin homografts in rats. Ann. N. Y. Acad. Sci., 120:119, 1964.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • H. K. Müller-Hermelink
    • 1
  • A. Thiede
    • 1
  • H.-G. Sonntag
    • 1
  • W. Müller-Ruchholtz
    • 1
  1. 1.Departments of Pathology, Surgery, and HygieneUniversity of KielGermany

Personalised recommendations