Abstract
Two morphologically different cell types are involved in the production of immunological sensitization against many antigens, namely, macrophages and lymphocytes (e.g., 4, 15, 22, 3). Applying antisera specifically directed against either of these cells may result in a delay or suppression of the primary immunological response. This has been proved to be true for antithymocyte sera (ATS) (e.g., 23, 8, 14, 1). Also, studies on the in vivo action of antimacrophage sera (AMS) revealed prolongation of allogeneic skin graft survival time in rats (17). The present work intends to elucidate morphologically whether or not the apparently similar effect of these antisera (prolongation of skin graft survival time) is caused by a specific action on different target cells (lymphocytes and macrophages) in vivo. Therefore, we studied the morphological alterations in popliteal lymph nodes following local application of either antiserum. The electron microscopic observations presented here are added to recently published light microscopic and enzyme histochemical studies (19, 20).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature
Barth, R. A., and J. Southworth Studies on heterologous antithymocyte sera. II. Effect on cellular antibody production during the early primary and secondary immune response of mice to sheep erythrocytes. J. Immunol., 101:1283, 1968.
Fiore-Donati, L., G. M. Cappuzzo, D. Collavo, N. Penelli, and L. Chieco-Bianchi. Morphological changes in lymphoid tissues of mice treated with antilymphocyte sera. In: Fiore-Donati, L., and M. G. Hanna, Jr. (eds.), Lymphatic tissue and germinal centers in immune response. Plenum Press, New York, p. 343, 1969.
Fishman, M. Induction of antibodies in vitro. Ann. Rev. Microbiol. 23:199, 1969.
Gallily, R., and M. Feldman. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology 12:197, 1967.
Gräsbeck, R., C. Nordman, and A. De la Chapelle. Mitogenic action of antileukocyte immune serum on peripheral leukocytes in vitro. Lancet II:385, 1963.
Grogan, J. B. Alterations in phagocytic function of rats after treatment with antilymphocyte serum. RES J. Reticuloendoth. Soc., 6:411, 1969.
Hirsch, M. S., G. W. Gram Jr., and F. A. Murphy. In vitro and in vivo properties of antimacrophage sera. J. Immunol., 102:656, 1968.
James, K. Anti-lymphocytic antibody. A review. Clin. exp. Immunol., 2:615, 1967.
Jennings, J. F., and L. A. Hughes. Inhibition of phagocytosis by antimacrophage antibodies. Nature 221:79, 1969.
Karnovsky, M. J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol., 27:137A, 1965.
Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. I. Evidence for opsonization. J. Immunol., 103:979, 1969.
Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. II. Nature and specificity of opsonizing antibody. J. Immunol., 103:990, 1969.
Martin, W. J. Assay for the immunosuppressive capacity of antilymphocyte serum. III. Opsonizing activity of antihuman lymphocyte serum. J. Immunol., 103:1000, 1969.
Medawar, P. B. Biological effects of heterologous antilymphocyte sera. In: Human transplantation. F. D. Rapaport and J. D. Dausset (eds.), New York and London, pp. 501, Grune and Stratton, 1968.
Mosier, D. E. A requirement for two cell types in antibody formation in vitro. Sci., 158:1573, 1967.
Riethmüller, G., D. Riethmüller, P. Rieber, and H. Stein. In vitro stimulation of lymphoid cells by antilymphocytic antibody. Bayer Symposium, Problems in Immunology, Springer, Berlin-Heidelberg-New York, 1969.
Sonntag, H.-G., and W. Mliller-Ruchholtz. Über den Einfluss auf die Überlebensdauer allogenetischer Rattenhaut-Transplantate. Vortag 2. Arbeitstag. Dtsch. Ges. Hyg. Mikrobiol., Mainz 1968.
Sonntag, H.-G., and W. Mliller-Ruchholtz. Autoref. Zbl. Bakt., I. Ref. 215:515, 1969.
Taub, R. N., and E. M. Lance. Histopathological effect in mice of heterologous antilymphocyte serum. J. Exper. Med., 128:1281, 1968.
Thiede, A., H.-G. Sonntag, and W. Müller-Ruchholtz. Unterschiedliche morphologische Veränderungen an Lymphknoten nach lokaler Applikation Heterologer Antithymocyten-und Antimakrophagen Seren. Virchows Arch., Abt. B, 3:302, 1969.
Thiede, A., H.-G. Sonntag, L.-D. Leder, H.-K. Müller-Hermelink, and W. Müller-Ruchholtz. Lymphknotenveränderungen nach lokaler Injektion heterologer antithymocyten und antimakrophagen seren. Verh. Dtsch. Ges. Path., 54, 1970.
Tridente, D., and D. W. Van Bekkum. Effect of antilymphocyteserum on mouse lymphoid tissues in vivo and in vitro. In: Fiore-Donati, L., and M. G. Hanna, Jr., (eds.), Lymphatic tissue and germinal centers in immune response. Plenum Press, New York, p. 371, 1969.
Unanue, E. R. Properties and some uses of antimacrophage antibodies. Nature, 218:36, 1969.
Woodruff, M. F. A., and N. F. Anderson. The effect of lymphocyte depletion by thoracic duct fistula and the administration of antilymphocytic serum on the survival of skin homografts in rats. Ann. N. Y. Acad. Sci., 120:119, 1964.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1971 Plenum Press, New York
About this chapter
Cite this chapter
Müller-Hermelink, H.K., Thiede, A., Sonntag, HG., Müller-Ruchholtz, W. (1971). Light and Electron Microscopic Changes in Lymph Nodes after Local Application of Heterologous Antithymocyte and Anti-Macrophage Serum. In: Di Luzio, N.R., Flemming, K.B.P. (eds) The Reticuloendothelial System and Immune Phenomena. Advances in Experimental Medicine and Biology, vol 15. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3204-6_26
Download citation
DOI: https://doi.org/10.1007/978-1-4684-3204-6_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-3206-0
Online ISBN: 978-1-4684-3204-6
eBook Packages: Springer Book Archive