Differential Responses of Macrophage Precursors and Antibody Forming Cells to Immunization
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Participation of macrophages in immune responses has been shown in many experiments using various methods (1) (2) (3) (4) (5). It has also been demonstrated that antibody forming cells and their precursors proliferate as a result of injection of antigen (6) (7). As for macrophages and their precursors, this fact has not yet been fully elucidated. The technique of cloning spleen, bone marrow and embryonic liver cells in soft agar (8) (9) (10) now enables quantitative studies of macrophages as well. In this technique certain cells from these hematopoietic organs can multiply in a soft agar medium to which conditioned medium is added (11). This leads to formation of macroscopic cell colonies of four types: macrophages, granulocytes, blast cells, and a mixture of macrophages and granulocytes (9) (12). It has been shown that macrophage colonies originate from a single precursor cell (11). Therefore each macrophage colony in the soft agar represents a precursor cell.
KeywordsSpleen Cell Soft Agar Secondary Response Macrophage Colony Plaque Form Cell
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