Electron-Microscopic Cytochemistry of the Cell Coat of Kupffer Cells in Rat Liver

  • J. J. Emeis
  • E. Wisse
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 15)


Animal cells have an external surface layer closely bound to the plasma membrane, called the cell coat (1) or glycocalyx (for a recent survey see ref.2). This coat is usually composed of acidic glycoproteins or mucopolysaccharides (3), and is thought to play an important role in surface interactions between cells and transport phenomena across the plasma membrane.


Kupffer Cell Alcian Blue Acidic Glycoprotein Cell Coat Glutaraldehyde Fixation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Rambourg A. and C.P. Leblond, Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat. J. Cell Biol., 32:27, 1967.PubMedCrossRefGoogle Scholar
  2. 2.
    Bennett H.S., The cell surface: components and configurations, in: Handbook of Molecular Cytology (A. Lima-de-Faria ed.), North Holland Publ. Co., Amsterdam, 1261, 1969.Google Scholar
  3. 3.
    Spiro R.G., Glycoproteins, their biochemistry, biology and role in human disease, New Engl. J. Med., 281:991, 1969.PubMedCrossRefGoogle Scholar
  4. 4.
    Wisse E. and W. Th. Daems, Fine structural study on the sinusoidal lining cells of rat liver, in: Mononuclear Phagocytes (R. van Furth ed.), Blackwell Sci. Publ. Co., Oxford, 201, 1970.Google Scholar
  5. 5.
    Wisse E. and W. Th. Daems, Differences between endothelial and Kupffer cells in rat liver, Proc. 7th Int. Congr. Electron Microscopy, Grenoble, III:57, 1970.Google Scholar
  6. 6.
    Wisse E., An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids, J. Ultrastruct. Res., 31:125, 1970.PubMedCrossRefGoogle Scholar
  7. 7.
    Morard J.-C., M. Pisam and A. Rambourg, Modifications ultrastructurales et histochimiques du “cell coat” (glycolemme) des néphrons papillaires au cours de la diurèse, C.R. Acad. Sci. (Paris), 268:2353, 1969.Google Scholar
  8. 8.
    Behnke O., Electron microscopical observations on the cell coating of human blood platelets, J. Ultrastruct. Res., 24:51, 1968.PubMedCrossRefGoogle Scholar
  9. 9.
    Tice L. W. and R.J. Barrnett, Diazophthalocyanins as reagents for fine structural cytochemistry, J. Cell Biol., 25:23, 1965.PubMedCrossRefGoogle Scholar
  10. 10.
    Rambourg A., An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope, J. Histochem. Cytochem., 15:409, 1967.PubMedCrossRefGoogle Scholar
  11. 11.
    Thiery J.-P., Mise en evidence des polysaccharides sur coupes fines en microscopie electronique, J. Microscopie, 6:987, 1967.Google Scholar
  12. 12.
    Wisse E., to be published.Google Scholar
  13. 13.
    Parks H. F. and A.D. Chiquoine, Observations on early stages of phagocytosis of colloidal particles by hepatic phagocytes of the mouse, Proc. 1st European Regional Conference Electron Microscopy, Stockholm, 154, 1956.Google Scholar
  14. 14.
    Hampton H.C., An electron microscopic study of the hepatic uptake and excretion of submicroscopic particles injected into the blood stream and into the bile duct, Acta Anat., 32:262, 1958.PubMedCrossRefGoogle Scholar
  15. 15.
    Carr I., The fine structure of the mammalian lymphoreticular system, Int. Rev. Cytol., 27:283, 1970.PubMedCrossRefGoogle Scholar
  16. 16.
    Behnke O. and T. Zelander, Preservations of intercellular substances by the cationic dye alcian blue in preparative procedures for electron microscopy, J. Ultrastruct. Res., 31:424, 1970.PubMedCrossRefGoogle Scholar
  17. 17.
    Törö I., P. Rusza and P. Röhlich, Ultrastructure of early phagocytic stages in sinus endothelial and Kupffer cells of the liver, Exptl. Cell Res., 26:601, 1962.PubMedCrossRefGoogle Scholar
  18. 18.
    Orci L., R. Pictet and Ch. Rouiller, Image ultrastructurale de pinocytose dans la cellule de Kupffer du foie de rat, J. Microscopie, 6:413, 1967.Google Scholar
  19. 19.
    Matter A., L. Orci, W.G. Forssmann and Ch. Rouiller, The stereological analysis of the fine structure of the“micropinocytosis vermiformis” in Kupffer cells of the rat, J. Ultrastruct. Res., 23:272, 1968.CrossRefGoogle Scholar
  20. 20.
    Röhlich P. and I. Törö, Uptake of chylomicron particles by reticular cells of mesenterial lymph nodes of the rat, Proc. 3rd European Regional Conference Electron Microscopy, Prague, 225, 1964.Google Scholar
  21. 21.
    Nossal G.J.V., A. Abbott and J. Mitchell, Antigens in immunity. XIV. Electron microscopic autoradiographic studies of antigen capture in the lymph node medulla, J. Exptl. Med., 127:263, 1968.CrossRefGoogle Scholar
  22. 22.
    Shea S.M. and M.J. Karnovsky, The cell surface and intercellular junctions in liver as revealed by lanthanum after fixation with glutaraldehyde with added alcian blue, J. Cell Biol., 43:129a, 1969 (abstract).Google Scholar
  23. 23.
    Jacques P.J., Endocytosis, in: Lysosomes in Biology and Pathology (J.T. Dingle and H.B. Pell eds), North Holland Publ. Co., Amsterdam, II:395, 1969.Google Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • J. J. Emeis
    • 1
  • E. Wisse
    • 1
  1. 1.Laboratory for Electron MicroscopyUniversity of LeidenThe Netherlands

Personalised recommendations