Abstract
Of the many chemical and physical changes that occur in blood during storage,1–5 the poststorage erythrocyte adenosine triphosphate (ATP) level has the highest reported correlation with poststorage erythrocyte red cell viability (R = 0.94).6–8Although the correlation was great enough to be of predictive value for the regression, it was obviously not applicable to the estimation of expected cell viability from the ATP data for an individual unit of stored blood, Furthermore, the study was limited to blood which had been stored in acid-citrate-dextrose (ACD) U.S.P. formula A, or in citrate-phosphate-dextrose (CPD)8 solutions for either 21 or 28 days. The studies reported herein were undertaken for two primary reasons: (1) to expand the total number of cases and the experimental conditions of blood storage, and (2) to evaluate possible applications of the relationship.
Supported by Research Grant AM-09919, from the National Institute of Arthritis and Metabolic Diseases.
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References
Rapoport, S.: Dimensional, osmotiC., and chemical changes of erythrocytes in stored blood. I. Blood preserved in sodium citrate, neutral, and acid-citrate-glucose (ACD) mixtures, J. Clin. Invest. 26:591, 1947.
Schechter, D. C., and Swan, H.: Biochemical alterations of preserved blood, Arch. Surg. 84:269, 1962.
Gabrio, B. W., and Huennekens, F. M.: The role of nucleoside phosphorylase in erythrocyte preservation, Biochim. et biophys. acta 18:585, 1955.
DeVerdier, C. H., Garby, L., Hjelm, M., and Hogman, C.: Adenine in blood preservation: Posttransfusion viability and biochemical changes, Transfusion 4:331, 1964.
Valeri, C. R., Mercado-Lugo, R., and Danon, D.: Relationship between osmotic fragility and in vivo survival of autologous deglycerolized resuspended red blood cells, Transfusion 5:267, 1965.
Brewer, G. J., and Powell, R. D.: The adenosine triphosphate content of glucose-6-phosphate dehydrogenase-deficient and normal erythrocytes, including studies of a glucose-6-phosphate dehydrogenase-deficient man with “elevated erythrocytic ATP,” J. Lab. ε. Clin. Med. 67:726, 1966.
Nakao, K., Wada, T., Kamiyama, T., Nakao, M., and Nagano, K.: A direct relationship between adenosine triphosphate level and in vivo viability of erythrocytes, Nature 194:877, 1962.
DeM, R. J., Brewer, George J., and Wiorkowski, J. J.: Studies on the preservation of human blood. II. The relationship of erythrocyte adenosine triphosphate levels and other in vitro measures to red cell storageability, J. Lab. ε Clin. Med. 69:968, 1967.
Button, L. N., Gibson, J. G. II, and Walter, C. W.: Simultaneous determination of the volume of red cells and plasma for survival of stored blood, Transfusion 5:143, 1965.
Dern, R. J.: Studies on the preservation of human blood. III, The posttransfusion survival of stored and damaged erythrocytes in healthy donors and patients with severe trauma, J. Lab. ε Clin. Med. 71:254, 1968.
Beutler, E., and Baluda, M. C.: Simplified determination of blood adenosine triphosphate using the firefly system, Blood 23:688, 1964.
Dixon, W. J., editor: Biomedical computer programs, Health Sciences Computing Facilities, Department of Preventive Medicine and Public Health, School of Medicine, University of California, Los Angeles, 1964.
Kendall, M. G., and Stuart, A.: Advanced theory of statistics, ed. 3, New York, 1960, Hafner Publishing Company, Inc., vol. 2, p. 356.
Dern, R. J., Matsuda, T., and Wiorkowski, J. J.: Studies on the preservation of human blood. V. The effect of mixing of blood during storage on postinfusion red cell survival. (In press J. Lab. ε Clin, Med.).
Dern, R. J., Gwinn, R. P., and Wiorkowski, J. J.: Studies on the preservation of human blood, I. Variability in erythrocyte storage characteristics among healthy donors, J. Lab. ε Clin. Med. 67:955, 1966.
Dern, R. J. and Wiorkowski, J. J.: Studies on the preservation of human blood. IV. The hereditary component of pre-and poststorage erythrocyte adenosine triphosphate levels, J. Lab. ε Clin. Med. 73:1019, 1969.
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Dern, R.J. (1970). The Prediction of Poststorage Red Cell Viability From ATP Levels. In: Brewer, G.J. (eds) Red Cell Metabolism and Function. Advances in Experimental Medicine and Biology, vol 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-3195-7_19
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DOI: https://doi.org/10.1007/978-1-4684-3195-7_19
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