Abstract
The need for a physiological change in mammalian spermatozoa in the female genital tract as a prerequisite for fertilization was first recognized by Austin (1951) and Chang (1951) in the rabbit, and the change was termed “capacitation” (Austin, 1952). They found that if epididymal or ejaculated spermatozoa were introduced into the oviduct of the female rabbit before ovulation, eggs were fertilized. However, when the spermatozoa were introduced into the female after ovulation, few-if any-eggs were fertilized. In other words, the eggs lost the ability to be fertilized before the spermatozoa became capacitated.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Reference
Abney, T. O., and Skipper, J. K. (1969), Studies on sperm capacitation in a Millipore filter, J. Reprod. Fertil 18:159.
Adams, C. E., and Chang, M. C. (1962), Capacitation of rabbit spermatozoa in the Fallopian tube and in the uterus, J. Exp. Zool. 151:159.
Allison, A. C, and Hartree, E. F. (1970), Lysosomal enzymes in the acrosome and then-possible role in fertilization, J. Reprod. Fertil. 21:501.
Austin, C. R. (1951), Observations on the penetration of the sperm into the mammalian egg, Aust. J. Sei. Res. B 4:581.
Austin, C. R., (1952), The capacitation of mammalian sperm,Nature (Lond.) 170:326.
Austin, C. R., (1968), Ultrastructure of Fertilization, Holt, Rinehart, and Winston, New York.
Austin, C. R., and Bishop, M. W. H. (1958), Role of the rodent acrosome and perforatorium in fertilization, Proc. Roy. Soc. Lond. Ser. B 149:241.
Austin, C. R., Bavister, B. D., and Edwards, R. G. (1973), Components of capacitation, pp. 247, in: The Regulation of Mammalian Reproduction (S. J. Segal, R. Crozier, P. A. Corfman, and P. E. Condliffe, eds.), Charles C Thomas, Springfield, Ill.
Barret, A. J. (1972), Lysosomal enzymes in: Lysosomes, A Laboratory Handbook (J. T. Dingle, ed.), North-Holland, Amsterdam.
Barros, C. (1968a), In vitro capacitation of golden hamster spermatozoa, Anat. Rec. 160: 310.
Barros, C. (1968b), In vitro capacitation of golden hamster spermatozoa with Fallopian tube fluid of the mouse and rat, J. Reprod. Fertil. 17:203.
Barros, C, and Austin, C. R. (1967), In vitro fertilization and the sperm acrosome reaction in the hamster, J. Exp. Zool. 166:311.
Barros, C, and Garavagno, A. (1970), Capacitation of hamster spermatozoa with blood sera, J. Reprod. Fertil. 22:381.
Barros, C, and Yanagimachi, R. (1972), Polyspermy-preventing mechanisms in the golden hamster egg, J. Exp. Zool. 180:251.
Barros, C, Bedford, J. M., Franklin, L. E., and Austin, C. R. (1967), Membrane vesiculation as a feature of the mammalian acrosome reaction, J. Cell Biol. 34:C1.
Barros, C, Arrau, J., and Herrera, E. (1972a), Induction of the acrosome reaction of golden hamster spermatozoa with blood serum collected at different stages of the oestrous cycle,J. Reprod. Fertil. 28:67.
Barros, C, Vliegenthart, A. M., and Franklin, L. E. (1972b), Polyspermie fertilization of hamster eggs in vitro, J. Reprod. Fertil. 28:111.
Barros, C, Berrios, M., and Herrera, E. (1973a), Capacitation in vitro of guinea pig spermatozoa in a saline solution,J. Reprod. Fertil. 34:547
Barros, C, Fujimoto, M., and Yanagimachi, R. (1973b), Failure of zona penetration of hamster spermatozoa after prolonged preincubation in a blood serum fraction, J. Reprod. Fert. 55:89.
Bavister, B. D. (1969), Environmental factors important for in vitro fertilization in the hamster,J. Reprod. Fertil 18:544.
Bedford, J. M. (1967), Experimental requirements for capacitation and observations on ultrastructural changes in rabbit spermatozoa during fertilization, J. Reprod. Fertil. (suppl.) 2:35.
Bedford, J. M. (1968), Ultrastructural changes in the sperm head during fertilization in the rabbit, Am. J. Anat. 123:329.
Bedford, J. M. (1969a), Limitations of the uterus in the development of the fertilizing ability (capacitation) of spermatozoa,J. Reprod. Fertil. (suppl.) 8:19.
Bedford, J. M. (1969b), Morphological aspects of sperm capacitation in mammals, in: Advances in Biosciences, Vol. 4, Schering Symposium on Mechanisms Involved with Conception. Berlin, 36 pp., Pergamon Press, New York.
Bedford, J. M. (1970a), Sperm capacitation and fertilization in mammals, Biol. Reprod. (suppl.) 2:128.
Bedford, J. M. (1970b), The saga of mammalian sperm from ejaculation to syngatny, in: Sonderdruck aus 21, Colloquium der Gessellschaft fur Biologische Chemie, April,Mosbach, Bade, Germany, 124 pp., Springer-Verlag, Berlin.
Bedford, J. M. (1972), An electron microscopic study of sperm penetration into the rabbit egg after natural mating, Am. J. Anat. 133:213.
Bedford, J. M., and Shalkovsky, S. (1967), Species specificity of sperm capacitation in therabbit,J. Reprod. Fertil. 13:361.
Brinster, R. L. (1965), Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources, J. Reprod. Fertil. 10:227.
Caravaglios, R., and Cilotti, R. (1957), A study of the proteins in the follicular fluid of the cow, J. Endocrinol. 15:213.
Chang, M. C. (1951), Fertilizing capacity of spermatozoa deposited into the Fallopian tubes, Nature (Lond.) 168:691.
Chang, M. C. (1955), Development of fertilizing capacity of rabbit spermatozoa in the uterus, Nature (Lond.) 175:1036.
Chang, M. C. (1958), Capacitation of rabbit spermatozoa in the uterus with special reference to the reproductive phases of the female, Endocrinology 63:619.
Chang, M. C, and Sheaffer, D. (1957), Number of spermatozoa ejaculated at copulation, transported into the female, and present in the male tract of golden hamster, J. Hered.48:101.
Chang, M. C, Hanada, A., and Hunt, D. M. (1971), Fertilization of denuded rabbit eggs in vitro by sperm recovered from the uterus or vagina, Nature (Lond.) 232:343.
Conway, A. F., and Metz, C. B. (1971), A possible role of phospholipase in membrane fusion, in: Proceedings of the 11th Annual Meeting of the American Society of CellBiologists, p. 64.
Dan, J. C. (1954), Studies on the acrosome. II. Acrosome reaction in starfish spermatozoa, Biol. Bull. 107:203.
Dan, J. C. (1956), The acrosome reaction,Int. Rev. Cytol. 5:365.
Fawcett, D., and Hollenberg, R. D. (1963), Changes in the acrosome of guinea pig spermatozoa during passage through the epididymis, Z. Zellforsch. 60:216.
Franklin, L. E., Barros, C, and Fussell, E. N. (1970), The acrosomal region and the acrosome reaction in sperm of the golden hamster, Biol. Reprod. 5:180.
Gordon, M. (1973), Localization of phosphatase activity on the membrane of the mam malian sperm head, J. Exp. Zool. 8: 185
Gwatkin, R. B. L., and Andersen, O. F. (1969), Capacitation of hamster spermatozoa by bovine follicular fluid, Nature (Lond.) 224:1111
Hamner, C. E., and Sojka, N. J. (1967), Capacitation of rabbit spermatozoa; species and organ specificity, Proc. Soc. Exp. Biol Med. 224:689.
Hamner, C. E., and Sojka, N. J. (1968), Requirements for capacitation of rabbit spermatozoa, Nature (Lond.) 220:1042.
Iwamatsu, T., and Chang, M. C. (1969), In vitro fertilization of mouse eggs in the presence of bovine follicular fluid, Nature (Lond.) 224:919.
Iwamatsu, T., and Chang, M. C. (1970), Further investigation of capacitation of sperm and fertilization of mouse eggs in vitro, J. Exp. Zool. 175:211.
Iwamatsu, T., and Chang, M. C. (1971), Factors involved in the fertilization of mouse eggs in vitro, J. Reprod. Fertil. 26:191
Iwamatsu, T., and Chang, M. C. (1972), Capacitation of hamster spermatozoa treated with rabbit tubal fluid or rabbit and bovine tubal cystic fluids, J. Exp. Zool. 182:211.
Lewis, B. K., and Ketchel, M. M. (1973), Effects of female reproductive tract secretions on rabbit sperm. III. Acrosomal enzyme changes related to capacitation, Proc. Soc. Exp. Biol. Med. 143:540.
Mahi, C, and Yanagimachi, R. (1973), The use of incubation temperature as a means of separating capacitation processes in Syrian hamster spermatozoa, Biol. Reprod. 9:13.
Mancini, R. E., Alousa, A., Barquet, J., Alvereg, B., and Neminosky, M. (1964), Histo immunological localization of hyaluronidase in the bull testis, J Reprod. Fertil. 8:325.
Martan, J., and Shepherd, B. A. (1973), Spermatozoa in rouleaux in the female guinea pig genital tract, Anat. Rec. 175:625.
Miyamoto, H., and Chang, M. C. (1973), The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro, J. Reprod. Fertil. 32:193.
Noyes, R. W., Walton, A., and Adams, C. A. (1958), Capacitation of rabbit spermatozoa,J. Endocrinol. 17:314
Piko, L. (1969), Gamete structure and sperm entry in mammals, in: Fertilization, Vol. II, Chapter 18 (C. B. Metz and A. Monroy, eds.), Academic Press, New York.
Rogers, B. J., and Morton, B. (1973), The release of hyaluronidase from capacitating hamster spermatozoa,J. Reprod. Fertil, 35:477
Simeone, F. A., and Young, W. C. (1931), A study of the function of the epididymis. IV. The fate of nonejaculated spermatozoa in the genital tract of the male guinea pig, J. Exp. Biol. 5:163.
Soupart, P. (1967), Studies on the hormonal control of rabbit sperm capacitation, J. Reprod. Fertil. (suppl) 2:49.
Soupart, P., and Orgebin-Crist, M. C. (1966), Capacitation of rabbit spermatozoa delayed in vivo by double ligation of uterine horn, J. Exp. Zool. 163:311.
Srivastava, P. N. (1973), Location of the zona lysin, Biol Reprod. 9:84.
Strauss, F. (1956), The time and place of fertilization of the golden hamster egg, J. Embryol Exp. Morphol 4:42.
Talbot, P., and Franklin, L. E. (1974), Hamster hyaluronidase. II. Its release from sperm in vitro in relation to changes in the acrosome, J. Exp. Zool, in press.
Toyoda, Y., Yokoyama, M., and Hoshi, T. (1971), Studies on the fertilization of mouse eggs in vitro. I. In vitro fertilization of eggs by fresh epididymal sperm, Jap. J. Anim. Reprod. 16:152.
Williams, W. L., Hamner, C. E., Weinman, D. E., and Brackett, B. G. (1964), Capacitation of rabbit spermatozoa and initial experiments on in vitro fertilization, in: Fifth International Congress on Reproduction, Trento, Vol. 7, p. 288.
Yanagimachi, R. (1966), Time and process of sperm penetration into hamster ova in vivo and in vitro, J. Reprod. Fertil. 11:359.
Yanagimachi, R. (1969a),In vitro capacitation of hamster spermatozoa by follicular fluid, J. Reprod. Fertil 18:215.
Yanagimachi, R. (1969b), In vitro acrosome reaction and capacitation of golden hamster spermatozoa by bovine follicular fluid and its fractions, J. Exp. Zool 170:269.
Yanagimachi, R. (1970a), The movement of golden hamster spermatozoa before and after capacitation,J. Reprod. Fertil. 23:193.
Yanagimachi, R. (1970b), In vitro capacitation of golden hamster spermatozoa by homologous and heterologous blood sera, Biol. Reprod. 3:141.
Yanagimachi, R. (1972a),In vitro fertilization of guinea pig ova,Anat. Rec. 172:430.
Yanagimachi, R. (1972b), Penetration of guinea pig spermatozoa into hamster eggs in vitro, J. Reprod. Fertil. 28:411.
Yanagimachi, R. (1973), Behavior and function of the ultrastructural elements of the mammalian sperm head in fertilization, in: The Regulation of Mammalian Reproduction, pp. 215 (S. J. Segal, R. Crozier, P. A. Corfman, and P. G. Codliffe, eds.), Charles C Thomas, Springfield, Ill.
Yanagimachi, R., and Chang, M. C. (1963), Sperm ascent through the oviduct of the hamster and rabbit in relation to the time of ovulation, J. Reprod. Fertil. 6:413.
Yanagimachi, R., and Chang, M. C. (1964), In vitro fertilization of golden hamster ova, J. Exp. Zool. 156:361.
Yanagimachi, R., and Noda, Y. D. (1970), Ultrastructural changes in the hamster spermhead during fertilization,J. Ultrastruct. Res. 31:465.
Yanagimachi, R., and Teichman, R. J. (1972), Cytochemical demonstration of acrosomalproteinase in mammalian and avian spermatozoa by a silver proteinate method, Biol. Reprod. 6:87.
Zaneveld, L. J. D., Dragoje, B. M., and Schumacher, G. F. B. (1972a), Acrosomal proteinase and proteinase inhibitor of human spermatozoa, Science 177:102.
Zaneveld, L. J. D., Polakoski, K. L., and Williams, W. L. (1972b), Properties of proteolitic enzyme from rabbit sperm acrosomes, Biol. Reprod. 6:30.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1974 Springer Science+Business Media New York
About this chapter
Cite this chapter
Barros, C. (1974). Capacitation of Mammalian Spermatozoa. In: Coutinho, E.M., Fuchs, F. (eds) Physiology and Genetics of Reproduction. Basic Life Sciences, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2892-6_1
Download citation
DOI: https://doi.org/10.1007/978-1-4684-2892-6_1
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-2894-0
Online ISBN: 978-1-4684-2892-6
eBook Packages: Springer Book Archive