The Preparation and Analysis of Myelin from Small Quantities of Central Nervous Tissue: Regional Studies of the Quaking Mouse

  • G. E. Fagg
  • T. V. Waehneldt
  • V. Neuhoff
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 100)


Myelin of considerable purity may be isolated from small (minimum 1 mg wet weight) samples of central nervous tissue, using a 4-step centrifugation procedure. The separation of myelin proteins by micro-linear gradient polyacrylamide gel electrophoresis yields similar results to those obtained by macro-scale (homogeneous) gel systems. These techniques have been employed for a preliminary study of the regional composition of myelin fractions from the Quaking mouse.


Protein Composition Myelin Protein Central Nervous System Region Central Nervous Tissue Small Tissue Sample 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • G. E. Fagg
    • 1
  • T. V. Waehneldt
    • 1
  • V. Neuhoff
    • 1
  1. 1.Forschungsstelle NeurochemieMax-Planck-Institut für Experimentelle MedizinGöttingenGermany

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