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Studies on Myelin Proteins in Human Peripheral Nerve

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Myelination and Demyelination

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 100))

Summary

The myelin fraction from human peripheral nerve was prepared. Two basic protein fractions (BF-P2 and PB) were isolated from acid extracts of the myelin fraction and three glycoproteins(BR-PO, PASII and Y protein) were purified from its acid-insoluble residue. In biochemical analysis the human BF-P2 protein (M.W.13,000) showed similar but not identical properties to bovine BF-P2 protein. The PB fraction was suggested to include the encephalitogenic CNS-BP (M.W.18,000) and another, new protein of similar molecular weight.

Both the human BR-PO protein (M.W.28,000) and PASII protein (M.W.13,000) showed similar biochemical properties to the corresponding myelin proteins of bovine peripheral nerve, while they both are clearly different from other myelin proteins. Close relationship between the BR-PO protein and the Y protein (M.W.22,000) was suggested by amino acid analysis.

Injection of the myelin fraction of bovine peripheral nerve with the complete adjuvant produced EAN while the CNS-BP induced EAE in laboratory animals. However, all three purified proteins. BF-P2, BR-PO and PASII, from bovine peripheral nerve myelin were inactive in inducing demyelinating diseases.

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Abbreviations

CNS:

Central nervous system

PNS:

Peripheral nervous system

CNS-BP:

Encephalitogenic basic protein (M.W.18,000) in CNS myelin

FLPL:

Folch-Lees type proteolipid in CNS myelin

BF-P2:

protein — Basic protein (M.W.13,000) in PNS myelin

BR-PO:

protein — Glycoprotein (M.W.28,000) in PNS myelin

PASII:

Protein — Glycoprotein (M.W.13,000) in PNS myelin

Y:

Protein — Glycoprotein (M.W.22,000) in PNS myelin

SDS:

Sodium dodecyl sulfate

PAS:

Periodic acid-Shiff

EAN:

Experimental allergic neuritis

EAE:

Experimental allergic encephalomyelitis

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Authors and Affiliations

  1. Department of Physiology, Saitama Medical School, Moroyama, Irumagun, Saitama, Japan

    Keiichi Uyemura, Masaru Suzuki & Kunio Kitamura

Authors
  1. Keiichi Uyemura
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  2. Masaru Suzuki
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  3. Kunio Kitamura
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Editors and Affiliations

  1. University of Helsinki, Helsinki, Finland

    Jorma Palo

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© 1978 Plenum Press, New York

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Uyemura, K., Suzuki, M., Kitamura, K. (1978). Studies on Myelin Proteins in Human Peripheral Nerve. In: Palo, J. (eds) Myelination and Demyelination. Advances in Experimental Medicine and Biology, vol 100. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2514-7_7

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  • DOI: https://doi.org/10.1007/978-1-4684-2514-7_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-2516-1

  • Online ISBN: 978-1-4684-2514-7

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