Isoelectric Focusing and Isotachophoresis for Investigation of CSF and Serum Proteins in Demyelinating and Infectious Neurological Diseases
- 67 Downloads
Isoelectric focusing (IEF) and isotachophoresis (ITP), two methods with excellent separation capacities, have been adapted during recent years for the analysis of CSF proteins. The fractions separated by these techniques can be further studied by e.g. immunological methods. ITP has besides its high separation capacity several valuable advantages: very small samples are needed, unconcentrated CSF can be examined, the analyses are quickly performed and the results are immediately obtained on a recorder.
Examinations by thin-layer IEF in a series of about 2,000 patients have afforded much new information about the CSF and serum proteins in many neurological diseases. Different complex CSF protein aberrations have been found in the gammaglobulin range as well as in more anodal positions in MS, infectious neurological diseases and Guillain-Barré syndromes. These aberrations are probably the result of several interacting factors, e.g.the temporal and spatial characteristics of the disease, the release of decomposition products from destroyed tissues, the genetically determined reactivity of the individual and the type of etiological agent.
KeywordsMultiple Sclerosis Spinal Muscular Atrophy Optic Neuritis Abnormal Band Cerebrospinal Fluid Protein
Unable to display preview. Download preview PDF.
- 1.Arbuthnott, J.P. and Beeley, J.A. (Eds.): Isoelectric focusing, London, Butterworths (1975).Google Scholar
- 2.Davies, H., Thin-layer gel isoelectric focusing, in Isoelectric focusing (J.P. Arbuthnott and J.A. Beeley, eds.) London, Butterworths (1975) pp. 97–113.Google Scholar
- 3.Delmotte, P., Gel isoelectric focusing of cerebrospinal fluid proteins: A potential diagnostic tool, Z. klin. Chem. u. klin. Biochem. 9 (1971) 334–336.Google Scholar
- 7.Hallander, L.B. and Kjellin, K.G., Isotachophoresis for investigation of cerebrospinal fluid proteins, in Abstracts of the 6th International Meeting of the ISN, Copenhagen, August 1977.Google Scholar
- 9.Kjellin, K.G. and Vesterberg, 0., Thin-layer isoelectric focusing of cerebrospinal fluid proteins. A preliminary report with special reference to the diagnostic significance in multiple sclerosis. In Proceedings of the 20th Congress of Scandinavian Neurologists, Oslo 1972, pp. 379–380.Google Scholar
- 11.Kjellin, K.G., Moberg, U. and Hallander, L., Analytical isotachophoresis of cerebrospinal fluid proteins–a preliminary report, Sci. Tools 22 (1975) 3–7.Google Scholar
- 15.Kjellin, K.G. and Sidén, A., Electrofocusing and electrophoresis of cerebrospinal fluid proteins in CNS disorders of known or probable infectious etiology, Eur. Neurol., in press.Google Scholar
- 16.Kurtzke, J.F., Diagnosis and differential diagnosis of multiple sclerosis, Acta Neurol. Scand. 46 (1970) 484–492.Google Scholar
- 18.Link, H. and Müller, R., Immunoglobulins in multiple sclerosis and infections of the nervous system, Arch. Neurol. 25 (1971) 326–344.Google Scholar
- 20.Lowenthal, A., Agar Gel Electrophoresis in Neurology, Amsterdam, Elsevier Publishing Company(1964).Google Scholar
- 21.Righetti, P.G. (Ed.), Progress in Isoelectric Focusing and Isotachophoresis. Amsterdam, North-Holland Publishing Company (1975).Google Scholar
- 22.Sidén, A., Crossed immunoelectrofocusing of cerebrospinal fluid immunoglobulins, J. Neurol., in press.Google Scholar
- 25.Söderholm, J. and Smyth, C.J., Crossed immunoelectrofocusing for studies on protein microheterogeneity, in Progress in Isoelectric Focusing and Isotachophoresis ( P.G. Righetti, ed.) Amsterdam; North-Holland Publishing Company (1975) pp. 99–114.Google Scholar
- 26.Thompson, E.J., Laboratory diagnosis of multiple sclerosis: immunological and biochemical aspects, Br. Med. Bull. 35 (1977) 28–33.Google Scholar
- 27.Ursing, B., Clinical and immunoelectrophoretic studies of cerebrospinal fluid in virus meningoencephalitis and bacterial meningitis, Acta Med. Scand. suppl. 429 (1965).Google Scholar
- 28.Vesterberg, 0., Some aspects of isoelectric focusing in polyacrylamide gel, in Isoelectric Focusing (J.P. Arbuthnott and J.A. Beeley, eds.) London, Butterworths (1975) pp. 78–96.Google Scholar