Abnormal Myelin Maturation In Vitro: the Role of Cerebrosides

  • Keith Bradbury
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 100)


Myelinating neonatal rat cerebellar explants were maintained for up to 130 days in vitro. Myelin fractions were extracted from explants of different ages and purified by density gradient centrifugation. The three fractions obtained were termed “light myelin”, “heavy myelin” and “membrane fraction” and were deficient in glycolipids compared to myelin fractions prepared from 15-day-old rat cerebellum. The culture myelin has an apparently normal ultra-structure but may not be as stable as myelin with a normal glycolipid composition.


Multiple Sclerosis Membrane Fraction Myelinated Axon Myelin Membrane Chick Embryo Extract 
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  1. 1.
    Agrawal, H.C., Banik, N.L., Bone, A.H., Davison, A.N., Mitchell, R.F. and Spohn, M., The identity of a myelin-like fraction isolated from developing brain, Biochem. J. 120 (1970) 635–642.PubMedGoogle Scholar
  2. 2.
    Agrawal, H.C., Trotter, J.L, Burton, R.M. and Mitchell, R.F., Metabolic studies on myelin. Evidence for a precursor role of a myelin subfraction, Biochem. J. 140 (1974) 99–109.PubMedGoogle Scholar
  3. 3.
    Aparicio, S.R., Bradbury, K., Bradbury, Margaret and Howard, L., Organ cultures of nervous tissues, in Organ culture in biomedical research. British Society for Cell Biology Symposium I ( M. Balls and M.A. Monnickendam, eds.) Cambridge University Press, Cambridge (1976) pp. 309–354.Google Scholar
  4. 4.
    Carter, H.E. and Gaver, R.C., Improved reagent for trimethylsilylation of sphingolipid bases, J. Lipid Res. 8 (1967) 391–395.PubMedGoogle Scholar
  5. 5.
    Eng, L.F., Chao, F.-C., Gerstl, B. Pratt, D. and Tavaststjerna, M.G., The maturation of human white matter myelin. Fractiona-tion of the myelin membrane proteins, Biochemistry (Wash.) 7 (1968) 4455–4465.CrossRefGoogle Scholar
  6. 6.
    Eng, L.F. and Noble, E.P., The maturation of rat brain myelin, Lipids 3 (1968) 157–162.PubMedCrossRefGoogle Scholar
  7. 7.
    Folch, J., Lees, M. and Sloane-Stanley, G.H., A simple method for the isolation and purification of total lipides from animal tissues, J. Biol. Chem. 226 (1957) 497–509.PubMedGoogle Scholar
  8. 8.
    Horrocks, L.A., Composition of mouse brain myelin during development, J. Neurochem. 15 (1968) I83–488.CrossRefGoogle Scholar
  9. 9.
    Kurihara, T. and Tsukada, Y., The regional and subcellular distribution of 2’,3’-cyclic nucleoside 3’-phosphohydrolase in the central nervous system, J. Neurochem. 14 (1967)1167–1174.Google Scholar
  10. 10.
    Lehrer, G.M., The tissue culture as a model for the biochemistry of brain development, in Progress in Brain Research, Vol. 40: Neurobiological aspects of maturation and aging ( D.H. Ford, ed.) Elsevier, Amsterdam (1973) pp. 219–230.CrossRefGoogle Scholar
  11. 11.
    Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., Protein measurement with Folin phenol reagent, J. Biol. Chem. 193 (1951) 265–275.PubMedGoogle Scholar
  12. 12.
    Lumsden, C.E., Nervous tissue in culture, in The structure and function of nervous tissue, Vol. I: Structure I. ( G.H. Bourne, ed.) Academic Press, New York (1968) pp. 67–140.Google Scholar
  13. 13.
    Murray, Margaret R., Nervous tissue in vitro, in Cells and tissues in culture. Methods, Biology and Physiology, vol. 2 ( E.N. Willmer,ed.) Academic Press, London (1965) pp. 373–455.Google Scholar
  14. 14.
    Peterson, E.R., Production of myelin sheaths in vitro by embryonic spinal ganglion cells, Anat. Rec. 106 (1950) 232.Google Scholar
  15. 15.
    Raine, C.S., Hummelgard, A., Swanson, E. and Bornstein, M.B., Multiple sclerosis: serum-induced demyelination in vitro. A light and electron microscopic study, J. Neurol. Sci. 20 (1974) 127–148.CrossRefGoogle Scholar
  16. 16.
    Rappaport, D.F. and Eichhorn, F., Valoracion clinica y mejoras al methodo Sidney Pearson y colaboradores para determinacion de cholesterol total, Revta med. Cordoba 43 (1955) 86–89.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Keith Bradbury
    • 1
  1. 1.Department of PathologyUniversity of LeedsLeedsEngland

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