Studies on the Action of Myelin Basic Protein (MBP) in Rat Brain
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The specificity of I125-MBP uptake and subcellular distribution on a discontinuous sucrose density gradient, compared to those of histone H 4 and cytochrome c, showed a high affinity of MBP for mitochondria and heavy synaptosomes, and of histone H 4 for lighter synaptosomes. One heavier synaptosomal subpopulation was almost equally labelled by both proteins. Cytochrome c showed only a low uptake into particular material. Receptor interaction studies of MBP with H3-labelled 5-hydroxytryptamine and naloxone gave negative results.
KeywordsMyelin Basic Protein Opiate Receptor Polytron Homogenizer Cortex Slice Discontinuous Sucrose Density Gradient
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