Skip to main content
SpringerLink
Log in

Quantitative Studies of the Islets of Nondiabetic Patients

  • Chapter
The Diabetic Pancreas

Abstract

In view of the fact that the islets of Langerhans are dispersed into numerous different-sized bodies within the pancreas, the various methods used to determine the number of islets, their volume, and A:B ratio has led to divergent results. Some investigators counted the islets in representative sections. Laguesse,1 by reconstructing a single pancreatic lobule, observed that the actual number of islets was considerably in excess of that obtained by counting them in representative sections. Bensley,2 using his staining procedure, emphasized that when such pancreatic fragments are carefully selected they are to the same extent representative of the part of the organ from which they are taken. He also postulated that a large number of pancreases must be examined in order to ensure a reasonable range of individual variations. Gepts3 warned that the estimation of weight or volume of islets presents a difficult problem and that the estimate should be considered as very rough. He further stated that as a result of errors in the methodology of sampling, quantitative methods are without value for the determination of the proportion of insular tissue in any given pancreas.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Laguesse, E.: Rev. Gen. Histol. 2:1, 1906.

    Google Scholar 

  2. Bensley, R. R.: Amer. J. Anat., 12:297, 1911.

    Article  Google Scholar 

  3. Gepts, W.: Ann. Soc. R. Sci. Mid. Nat. Brux., 10:34, 1957.

    Google Scholar 

  4. Clark, E.: Anat. Anz., 43:81, 1913.

    Google Scholar 

  5. Opie, E. L.: Bull. Johns Hopkins Hosp., 11:205, 1900.

    Google Scholar 

  6. Flint, J. M.: Arch. Anat. Entwckingsgesch., 27:61, 1903.

    Google Scholar 

  7. Heiberg, K. A.: Anat. Anz., 29:49, 1906.

    Google Scholar 

  8. Heiberg, K. A.: Anat. Anz., 37:545, 1910.

    Google Scholar 

  9. Sauerbeck, E.: Ergeb. allg. Pathol. Anat, 8:539, 1902.

    Google Scholar 

  10. Laguesse, E.: C. R. Soc. Biol, 58:504, 1905.

    Google Scholar 

  11. Pochon: Arch. Wissensch. Prakt. Tierh,34:581, 1908.

    Google Scholar 

  12. Maximow, A., and Bloom, W.: A Textbook of Histology 5th ed. W. B. Saunders, Philadelphia, 1948, p. 443.

    Google Scholar 

  13. Schaffer, J.: Lehrbuch der Histologie und Histogenese 3rd ed. Wilhelm Engelmann, Leipzig, 1933.

    Google Scholar 

  14. De Witt, L. M.: J. Exp. Med,8:193, 1906.

    Article  Google Scholar 

  15. Heiberg, K. A.: Ergeb. Anat. Entwickl.-Gesch, 19:948, 1909.

    Google Scholar 

  16. Gündisch, M.: Cluj. Med, 15:406, 1934.

    Google Scholar 

  17. Weichselbaum, A.: Sitzungsb. Akad. Math.-Naturw. K,119:73, 1910.

    Google Scholar 

  18. Susman, W.: J. Clin. Endocrinol, 2:97, 1942.

    Article  Google Scholar 

  19. Ogilvie, R. R.: Quart. J. Med,6:287, 1937.

    Google Scholar 

  20. Seyfarth, C.: Klin. Wochenschr, 3:1085, 1924.

    Article  Google Scholar 

  21. Nakamura, N.: Virchows Arch, 253:286, 1924.

    Article  Google Scholar 

  22. Wilms, C.: In: von Möllendorffs Handbuch der Mikroskopischen Anatomie des Menschen J. Springer, Berlin, 1939, p. 209.

    Google Scholar 

  23. Dubs: Quoted in von Möllendorffs Handbuch der Mikroskopischen Anatomie des Menschen J. Springer, Berlin, 1939, p. 209.

    Google Scholar 

  24. Gellé: Ergeb. Anat. Entwickl.-Gesch,20:1042, 1911.

    Article  Google Scholar 

  25. Bargmann, W.: von Möllendorffs Handbuch der Mikroskopischen Anatomie des Menschen J. Springer, Berlin, 1939, p. 209.

    Google Scholar 

  26. Hellman, B.: Acta Soc. Med. Upsal, 64:432, 1959.

    CAS  Google Scholar 

  27. Hellman, B.: Nature (London) 184:1498, 1959.

    Article  Google Scholar 

  28. Hellman, B.: Acta Soc. Med. Upsal, 64:461, 1959.

    CAS  Google Scholar 

  29. Burkhardt, L. W.: Virchows Arch, 296:655, 1936.

    Article  Google Scholar 

  30. Schulze, W.: Arch. Mikr. Anat, 56:491, 1900.

    Article  Google Scholar 

  31. Diamare, V.: Int. Mschr. Anat. Physiol, 16:155, 1899.

    Google Scholar 

  32. Lane, M. A.: Amer. J. Anat, 7:409, 1907.

    Article  Google Scholar 

  33. Bloom, W.: Anat. Rec, 49:363, 1931.

    Article  Google Scholar 

  34. Miller, R. A.: Endocrinology,31:535, 1942.

    Article  CAS  Google Scholar 

  35. Gomori, G.: Amer. J. Pathol 17:395, 1939.

    Google Scholar 

  36. Ferner, H.: Das Inselsystem des Pankreas Georg Thieme Verlag, Stuttgart, 1952, p. 17.

    Google Scholar 

  37. Ito, T., Takahashi, Y., Aoki, H., and Yamamoto, T.: Arch. Histol. Jap 21:415, 1961.

    Article  Google Scholar 

  38. Conklin, J. L.: Amer. J. Anat, 111:181, 1962.

    Article  PubMed  CAS  Google Scholar 

  39. Lomsky, R. F., Langer, F., and Vortel, V.: Nature (London), 223:618, 1969.

    Article  CAS  Google Scholar 

  40. Greider, M. H., and McGuigan, J. E.: Diabetes, 20:387, 1971.

    PubMed  CAS  Google Scholar 

  41. Pelletier, G., LeClerc, R., Arimara, A., and Schally, A. V.: J. Histochem. Cytochem, 23:699, 1975.

    Article  PubMed  CAS  Google Scholar 

  42. Orci, L., Baetens, D., Dubois, M. P., and Rufener, C.: Horm. Metab. Res, 7:400, 1975.

    Article  PubMed  CAS  Google Scholar 

  43. Mortimer, C. H., Turnbridge, W. M. G., Carr, D., Yeomans, L., Lind, T., Coy, D. H., Bloom, S. R., Kastin, A., Mallinson, C. N., Besser, G. M., Schally, A. V., and Hall, R.: Lancet,1:697, 1974.

    Article  PubMed  CAS  Google Scholar 

  44. Koerker, D. J., Ruch, W., Chideckel, E., Palmer, J., Goodner, C. J., Ensinck, J., and Gale, C. C.: Science,184:482, 1974.

    Article  PubMed  CAS  Google Scholar 

  45. Johnson, D. G., Ensinck, J. W., Koerker, D. J., Palmer, J., and Goodner, C. J.: Diabetes, Suppl. 1,23:374, 1974.

    Google Scholar 

  46. Iverson, J.: Scand J. Clin. Lab. Invest,33:125, 1974.

    Article  Google Scholar 

  47. Efendic, S., Luft, R., and Grill, V.: FEBS Lett, 42:169, 1974.

    Article  CAS  Google Scholar 

  48. Gomori, G.: Amer. J. Pathol,15:497, 1939.

    CAS  Google Scholar 

  49. Gomori, G.: Amer. J Clin. Pathol, 20:665, 1950.

    CAS  Google Scholar 

  50. Lazarus, S. S., and Volk, B. W.: The Pancreas in Human and Experimental Diabetes Grune & Stratton, New York, 1962, p. 262.

    Google Scholar 

  51. Kon, A.: Über die Silberreaktion der Zellen G. Fischer Verlag, Jena, 1933, p. 47.

    Google Scholar 

  52. Ferner, H.: Z. Mikr.-Anat. Forsch, 44:451, 1938.

    Google Scholar 

  53. Creutzfeldt, W.: Z. Zellforsch,34:280, 1949.

    Google Scholar 

  54. Creutzfeldt, W.: Beitr. Path. Anat, 133:113, 1953.

    Google Scholar 

  55. Bencosme, S. A.: Arch. Pathol, 53:87, 1952.

    Google Scholar 

  56. Hamperl, H.: Virchows Arch, 321:482, 1952.

    PubMed  CAS  Google Scholar 

  57. Hellweg, G.: Virchows Arch, 327:502, 1955.

    Article  PubMed  CAS  Google Scholar 

  58. Grimelius, L.: In: The Structure and Metabolism of the Pancreatic Islets Edited by S. E. Brolin, B. Hellman, and H. Knutson. Macmillan, New York, 1964, p. 99.

    Google Scholar 

  59. Volk, B. W., Goldner, M. G., and Crowley, H. F.: Metabolism, 4:491, 1955.

    PubMed  CAS  Google Scholar 

  60. Hellman, B., and Hellerström, C.: Z. Zellforsch. Mikrosk. Anat. Abt. Histochem,52:278, 1960.

    Article  CAS  Google Scholar 

  61. Hellman, B., and Hellerström, C.: In: Handbuch des Diabetes Mellitus Edited by E. F. Pfeiffer. J. F. Lehmanns Verlag, München, 1969, p. 89.

    Google Scholar 

  62. Hellerström, C., Hellman, B., Petersson, B., and Mm, G.: In: The Structure and Metabolism of the Pancreatic Islets Edited by S. E. Brolin, B. Hellman, and H. Knutson. Macmillan, New York, 1964, p. 117.

    Google Scholar 

  63. Thomas, T. B.: Amer. J. Anat, 62:31, 1937.

    Article  Google Scholar 

  64. Caramia, F., Munger, B. L., and Lacy, P. E.: Z. Zellforsch, 67:533, 1965.

    Article  PubMed  CAS  Google Scholar 

  65. Munger, B. L., Caramia, F., and Lacy, P. E.: Z. Zellforsch, 67:776, 1965.

    Article  PubMed  CAS  Google Scholar 

  66. Bencosme, S. A., Lazarus, S. S., and Liepa, E.: Proc Soc Exp. Biol. Med, 90:387, 1955.

    PubMed  CAS  Google Scholar 

  67. Lazarus, S. S., and Shapiro, S. H.: Anat. Rec, 169:487, 1971.

    Article  PubMed  CAS  Google Scholar 

  68. Fujita, T.: Arch. Histol. Jap,25:189, 1964.

    Article  PubMed  CAS  Google Scholar 

  69. Petersson, B., Hellerström, C., and Hellman, B.: Z. Zellforsch, 57:559, 1962.

    Article  PubMed  CAS  Google Scholar 

  70. Deconinck, J., Potvliege, P. R., and Gepts, W.: Diabetologia, 7:266, 1971.

    Article  PubMed  CAS  Google Scholar 

  71. Deconinck, J., van Assche, F., Potvliege, P. R., and Gepts, W.: Diabetologia, 8:326, 1972.

    Article  PubMed  CAS  Google Scholar 

  72. Solcia, E., Pearse, A. G. E., Grube, D., Kobayashi, S., Bussolati, G., Creutzfeldt, W., and Gepts, W.: Rend. Gastroenterol, 5:13, 1973.

    Google Scholar 

  73. Like, A. A., and Orci, L.: Diabetes,Supp. 2, 21:511, 1972.

    CAS  Google Scholar 

  74. Kimmel, J. R., Pollock, H. G., and Hazelwood, R. L.: Endocrinology,83:1323, 1968.

    Article  PubMed  CAS  Google Scholar 

  75. Kimmel, J. R., Pollock, H. G., and Hazelwood, R. L.: Fed. Proc, 30:1318, (Abst.), 1971.

    Google Scholar 

  76. Lin, T. M., and Chance, R. E.: Gastroenterology,82:852 (Abst.), 1972.

    Google Scholar 

  77. Lin, T. M., Chance, R. E., and Evans, D.: Gastroenterology, 64:865, 1973.

    Google Scholar 

  78. Lin, T. M., Evans, D. C., and Chance, R. E.: Gastroenterology,6:852, 1974.

    Google Scholar 

  79. Larsson, L.-I., Sundler, F., and Hakanson, R.: Cell. Tiss. Res, 156:167, 1975.

    Article  CAS  Google Scholar 

  80. Larsson, L.-I., Sundler, F., and Hakanson, R.: Diabetologia,12:211, 1976.

    Article  PubMed  CAS  Google Scholar 

  81. Hazelwood, R. E., Turner, S. D., Kimmel, J. R., and Pollock, H. G.: Gen. Comp. Endocrinol 21:485, 1973.

    Article  PubMed  CAS  Google Scholar 

  82. Schwartz, T. W., Rehfeld, J. F., Stadil, F., Larsson, L. J., Chance, R. E., and Moon, N.: Lancet, 1:1102, 1976.

    Article  PubMed  CAS  Google Scholar 

  83. Vale, W., Brazeau, G., Grant, A., Nussey, R., Burgus, J., Rivier, N., Ling, N., and Guillemin, C. R.: C. R. Acad. Sci, 275:2913, 1972.

    CAS  Google Scholar 

  84. Liljenquist, J. E., Bomboy, J. D., Lewis, S. B., Sinclair-Smith, B. C., Felts, P. W., Lacy, W. W., Crofford, O. B., and Liddle, W.: J Clin. Invest,53:190, 1974.

    Article  PubMed  CAS  Google Scholar 

  85. Pelletier, G., Leclerc, G., Arimura, A., and Schally, A. V.: J. Histochem. Cytochem, 23:699, 1975.

    Article  PubMed  CAS  Google Scholar 

  86. Orci, L., and Unger, R. H.: Lancet,2:1243, 1975.

    Article  PubMed  CAS  Google Scholar 

  87. Ferner, H.: Virchows Arch, 309:87, 1942.

    Article  Google Scholar 

  88. Hess, W.: Schweiz. Z. Pathol. Bakteriol, 9:46, 1946.

    PubMed  CAS  Google Scholar 

  89. Terbrüggen, A.: Virchows Arch, 315:407, 1948.

    Article  PubMed  Google Scholar 

  90. Hultquist, G. T., Dahlen, M., and Helander, C. G.: Schweiz. Z. Pathol. Bakteriol 11:570, 1948.

    Google Scholar 

  91. Gepts, W.: Ann. Soc. R. Sci. Med. Nat. Brux,10:5, 1957.

    PubMed  CAS  Google Scholar 

  92. Gepts, W.: Endocrinologie, 36:185, 1958.

    CAS  Google Scholar 

  93. Creutzfeldt, W., and Theodossiou, A.: Beitr. Pathol. Anat, 117:235, 1957.

    PubMed  CAS  Google Scholar 

  94. Gomori, G.: Arch. Pathol,36:217, 1943.

    CAS  Google Scholar 

  95. Maclean, N., and Ogilvie, R. R.: Diabetes, 4:367, 1955.

    PubMed  CAS  Google Scholar 

  96. Gepts, W.: In: Handbuch of Diabetes Mellitus Edited by E. F. Pfeiffer, J. F. Lehmann, Munich, Vol. II, 1971, p. 3.

    Google Scholar 

Download references

Author information

Author notes

  1. Bruno W. Volk

    Present address: University of California, Irvine, California, USA

  2. Klaus F. Wellmann

    Present address: Beekman Downtown Hospital, New York, New York, USA

Authors and Affiliations

  1. Isaac Albert Research Institute of the Kingsbrook Jewish Medical Center and, Downstate Medical Center, State University of New York, Brooklyn, New York, USA

    Bruno W. Volk & Klaus F. Wellmann

Authors
  1. Bruno W. Volk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Klaus F. Wellmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Isaac Albert Research Institute, Brooklyn, New York, USA

    Bruno W. Volk  & Klaus F. Wellmann  & 

  2. University of California, Irvine, California, USA

    Bruno W. Volk

  3. Beekman Downtown Hospital, New York, USA

    Klaus F. Wellmann

Rights and permissions

Reprints and permissions

Copyright information

© 1977 Plenum Press, New York

About this chapter

Cite this chapter

Volk, B.W., Wellmann, K.F. (1977). Quantitative Studies of the Islets of Nondiabetic Patients. In: Volk, B.W., Wellmann, K.F. (eds) The Diabetic Pancreas. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-2325-9_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4684-2325-9_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-2327-3

  • Online ISBN: 978-1-4684-2325-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation